Artículo
Abstract:
A novel electrochemical method for the recognition of different species of microorganisms has been developed. It consists of sampled DC voltammetry applied directly to microbial cultures or to the supernatants of these cultures obtained following centrifugation. Pt, Au, and glassy carbon working electrode materials were examined. Complete and replicated voltammograms obtained at gold working electrodes were processed by factor analysis to generate the scores plots that were used for pattern recognition of the different species. Three Gram-negative bacteria (E. aerogenes, P. vulgaris and E. coli), two Gram-positive bacteria (B. cereus and S. aureus) and one fungus (S. cerevisiae) were tested. The chemometric methods employed for data analysis produced clear groupings of replicate voltammograms for these organisms, allowing species identification.
Registro:
| Documento: | Artículo |
| Título: | A novel electrochemical method for the identification of microorganisms |
| Autor: | Corton, E.; Raffa, D.; Mikkelsen, S.R. |
| Filiación: | Department of Chemistry, University of Waterloo, Waterloo, Ont. N2L 3G1, Canada Department of Biological Chemistry, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina Department of Inorganic Chemistry, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina |
| Palabras clave: | Bacteria; Fungi; Microbial identification; Voltammetry; carbon; gold; platinum; article; bacterium culture; bacterium identification; centrifugation; chemometric analysis; electrochemical analysis; electrode; fungus; Gram negative bacterium; Gram positive bacterium; microorganism; nonhuman; pattern recognition; potentiometry |
| Año: | 2001 |
| Volumen: | 13 |
| Número: | 12 |
| Página de inicio: | 999 |
| Página de fin: | 1002 |
| DOI: | http://dx.doi.org/10.1002/1521-4109(200108)13:12<999::AID-ELAN999>3.0.CO;2-8 |
| Título revista: | Electroanalysis |
| Título revista abreviado: | Electroanalysis |
| ISSN: | 10400397 |
| CODEN: | ELANE |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10400397_v13_n12_p999_Corton |
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Citas:
---------- APA ----------
Corton, E., Raffa, D. & Mikkelsen, S.R. (2001) . A novel electrochemical method for the identification of microorganisms. Electroanalysis, 13(12), 999-1002.http://dx.doi.org/10.1002/1521-4109(200108)13:12<999::AID-ELAN999>3.0.CO;2-8
---------- CHICAGO ----------
Corton, E., Raffa, D., Mikkelsen, S.R. "A novel electrochemical method for the identification of microorganisms" . Electroanalysis 13, no. 12 (2001) : 999-1002.http://dx.doi.org/10.1002/1521-4109(200108)13:12<999::AID-ELAN999>3.0.CO;2-8
---------- MLA ----------
Corton, E., Raffa, D., Mikkelsen, S.R. "A novel electrochemical method for the identification of microorganisms" . Electroanalysis, vol. 13, no. 12, 2001, pp. 999-1002.http://dx.doi.org/10.1002/1521-4109(200108)13:12<999::AID-ELAN999>3.0.CO;2-8
---------- VANCOUVER ----------
Corton, E., Raffa, D., Mikkelsen, S.R. A novel electrochemical method for the identification of microorganisms. Electroanalysis. 2001;13(12):999-1002.http://dx.doi.org/10.1002/1521-4109(200108)13:12<999::AID-ELAN999>3.0.CO;2-8
