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Abstract:

Porphobilinogen deaminase was purified from human hepatocytes. A variety of group specific reagents have been used to achieve site-specific modifications to evaluate the potential role of such groups in the whole catalytic cycle. Treatment with dicarbonyl reagents caused a rapid loss in activity that was time and concentration dependent. Protection experiments revealed that arginine residues are involved in the binding of the substrate. Treatment with Woodward's reagent K showed the fastest inactivation of deaminase (85% in 30 sec at 30 mM) which was pH dependent and could be prevented by the presence of substrate, suggesting that deprotonated carboxylated groups from Asp/Glu are essential for catalytic activity. Kinetic analysis gave values of 0.3 sec-1 for the k3 rate constant and 8 x 10-2 M for the K(I) of the non covalent complex between deaminase-Woodward's Reagent K.

Registro:

Documento: Artículo
Título:Studies on the catalytic activity of human hepatic porphobilinogen deaminase.
Autor:Mazzetti, M.B.; María Tomio, J.
Filiación:Departmento de Quim. Biol., Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires. (1428), Argentina
Palabras clave:arginine; liver enzyme; porphobilinogen deaminase; article; catalysis; enzyme activity; enzyme inactivation; enzyme kinetics; human; human cell; liver cell
Año:1997
Volumen:42
Número:4
Página de inicio:685
Página de fin:692
Título revista:Biochemistry and Molecular Biology International
Título revista abreviado:Biochem. Mol. Biol. Int.
ISSN:10399712
CODEN:BMBIE
CAS:arginine, 1119-34-2, 15595-35-4, 7004-12-8, 74-79-3; porphobilinogen deaminase, 9036-47-9, 9074-91-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10399712_v42_n4_p685_Mazzetti

Referencias:

  • Jordan, P.M., (1991) New Compr. Biochem., 19, pp. 1-66
  • Frydman, R.B., Frydman, B., (1973) Biochim. Biophys. Acta, 293, pp. 506-513
  • Pollack, S.E., Russell, C.S., (1978) FEBS Lett., 90, pp. 47-50
  • Russell, C.S., Pollack, S., James, J., (1984) Ann. N.Y. Acad. Sci., 435, pp. 202-204
  • Mazzetti, M.B., Tomio, J.M., (1988) Biochim. Biophys. Acta, 957, pp. 97-104
  • Mazzetti, M.B., Tomio, J.M., (1989) An. Asoc. Quím. Argent, 77, pp. 71-78
  • Lander, M., Pitt, A.R., Alefounder, P.R., Bardy, D., Abell, C., Battersby, A.R., (1991) Biochem. J., 275, pp. 447-452
  • Jordan, P.M., Woodcock, S.C., (1991) Biochem. J., 280, pp. 445-449
  • Woodcock, S.C., Jordan, P.M., (1994) Biochem. J., 33, pp. 2688-2695
  • Mazzetti, M.B., Tomio, J.M., (1990) Biochem. Internat., 21, pp. 463-471
  • Mazzetti, M.B., Tomio, J.M., (1993) An. Asoc. Quím. Argent, 81, pp. 381-389
  • Kreimer-Birnbaum, M., Tomio, J.M., (1976) Porphyrin in Human Diseases, pp. 182-188. , (Doss M. ed.) S. Karger AG, Basel
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., (1951) J. Biol. Chem., 193, pp. 265-275
  • Mazzetti, M.B., Tomio, J.M., (1988) Acta Bioquím. Clín. Latinoam., 22, pp. 509-519
  • Kitz, R., Wilson, I.B., (1962) J. Biol.Chem., 237, pp. 3245-3249
  • Mazzetti, M.B., (1991), Doctoral Thesis, University of Buenos Aires; Louie, G.V., Brownlie, P.D., Lambert, R., Cooper, J.B., Blundell, T.L., Wood, S.P., Warren, M.J., Jordan, P.M., (1992) Nature (London), 359, pp. 33-39
  • Kappas, A., Sassa, S., Galbraith, R.A., Nordmann, Y., (1995) The Metabolic and Molecular Basis of Inherited Disease, 2, pp. 2103-2159. , (Scriver, C.R., Beaudet, A.L., Sly, W.S., Valle, D., Stanbury, J.B., Wyngaarden, J.B. and Fredrickson, D.S., eds. ) Mc Graw-Hill, New York
  • Brownlie, P.D., Lambert, R., Lowie, G.V., Jordan, P.M., Blundell, T.L., Warren, M.J., Cooper, J.B., Wood, S.P., (1994) Protein Science, 3, pp. 1644-1650
  • Kauppinen, R., Mustajoki, S., Pihlaja, H., Peltonen, L., Mustajoki, P., (1995) Hum. Mol. Genet., 4, pp. 215-222
  • Petra, P.H., (1971) Biochemistry, 10, pp. 3163-3170

Citas:

---------- APA ----------
Mazzetti, M.B. & María Tomio, J. (1997) . Studies on the catalytic activity of human hepatic porphobilinogen deaminase. Biochemistry and Molecular Biology International, 42(4), 685-692.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10399712_v42_n4_p685_Mazzetti [ ]
---------- CHICAGO ----------
Mazzetti, M.B., María Tomio, J. "Studies on the catalytic activity of human hepatic porphobilinogen deaminase." Biochemistry and Molecular Biology International 42, no. 4 (1997) : 685-692.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10399712_v42_n4_p685_Mazzetti [ ]
---------- MLA ----------
Mazzetti, M.B., María Tomio, J. "Studies on the catalytic activity of human hepatic porphobilinogen deaminase." Biochemistry and Molecular Biology International, vol. 42, no. 4, 1997, pp. 685-692.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10399712_v42_n4_p685_Mazzetti [ ]
---------- VANCOUVER ----------
Mazzetti, M.B., María Tomio, J. Studies on the catalytic activity of human hepatic porphobilinogen deaminase. Biochem. Mol. Biol. Int. 1997;42(4):685-692.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10399712_v42_n4_p685_Mazzetti [ ]