Artículo

Lanuza, G.M.; Saragüeta, P.E.; Bussmann, U.A.; Barañao, J.L. "Paradoxical effects of tumour necrosis factor-α on rat granulosa cell DNA synthesis" (2002) Reproduction, Fertility and Development. 14(3-4):133-139
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Abstract:

Tumour necrosis factor-α (TNF-α) has been proposed as an intraovarian modulator of granulosa cell function. The effect of TNF-α on DNA synthesis in cultured rat granulosa cells was examined. Tumour necrosis factor-α stimulated thymidine incorporation when added in the presence of transforming growth factor-β (TGF-β). In contrast, the co-mitogenic effect of follicle-stimulating hormone (FSH) and TGF-β was inhibited in a dose-dependent manner by TNF-α. Inhibition of FSH-dependent DNA synthesis by TNF-α was also found when cultures were co-stimulated with activin A. The inhibitory action of TNF-α on FSH-treated cultures was not associated with changes in cell viability. The inhibitory effects of TNF-α could not be solely explained by a decrease in cAMP levels, since TNF-α was also able to inhibit the stimulation by dibutyryl-cAMP and TGF-β on granulosa cell DNA synthesis. These results suggest that TNF-α regulation of granulosa cell growth is elicited either independently or downstream from gonadotrophin-induced cAMP production. The actions of TNF-α could be only partially mimicked by a cell-permeable analogue of ceramide, thus indicating that actions of this cytokine can not be fully ascribed to an activation of sphingomyelinase. Data presented here indicate that, in addition to its previously demonstrated inhibitory effects on gonadotrophin-induced cell differentiation, TNF-α may also exert a marked inhibition on hormonally stimulated immature granulosa cell proliferation. In contrast to this inhibitory action, this cytokine could amplify the mitogenic action of putative intraovarian growth regulators such as TGF-β. These observations add further support to the notion that TNF-α plays a physiological role as a paracrine modulator of follicle development and may be also relevant to the alteration of ovarian function during physiopathological processes.

Registro:

Documento: Artículo
Título:Paradoxical effects of tumour necrosis factor-α on rat granulosa cell DNA synthesis
Autor:Lanuza, G.M.; Saragüeta, P.E.; Bussmann, U.A.; Barañao, J.L.
Filiación:IByME - CONICET, Dept. de Química Biol., Facultad de Ciencias Exactas Natural, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:activin A; bucladesine; cell DNA; ceramide; cyclic AMP; cytokine; follitropin; gonadotropin; sphingomyelin phosphodiesterase; thymidine; transforming growth factor beta; tumor necrosis factor alpha; activin; ceramide; cyclic AMP; DNA; follitropin; inhibin; tumor necrosis factor alpha; amino acid metabolism; animal cell; article; cell culture; cell differentiation; cell function; cell growth; cell membrane permeability; cell viability; controlled study; DNA synthesis; enzyme activation; female; granulosa cell; growth regulation; hormonal regulation; inhibition kinetics; mitogenesis; molecular mimicry; nonhuman; ovary follicle development; ovary function; paracrine signaling; rat; animal; biosynthesis; cell division; comparative study; cytology; drug effect; drug interaction; metabolism; Sprague Dawley rat; Animalia; Activins; Animals; Cell Division; Cells, Cultured; Ceramides; Cyclic AMP; DNA; Drug Interactions; Female; Follicle Stimulating Hormone; Granulosa Cells; Inhibin-beta Subunits; Rats; Rats, Sprague-Dawley; Tumor Necrosis Factor-alpha
Año:2002
Volumen:14
Número:3-4
Página de inicio:133
Página de fin:139
DOI: http://dx.doi.org/10.1071/RD01103
Título revista:Reproduction, Fertility and Development
Título revista abreviado:Reprod. Fertil. Dev.
ISSN:10313613
CODEN:RFDEE
CAS:activin A; Activins, 104625-48-1; Ceramides; Cyclic AMP, 60-92-4; DNA, 9007-49-2; Follicle Stimulating Hormone, 9002-68-0; Inhibin-beta Subunits, 93443-12-0; Tumor Necrosis Factor-alpha
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10313613_v14_n3-4_p133_Lanuza

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Citas:

---------- APA ----------
Lanuza, G.M., Saragüeta, P.E., Bussmann, U.A. & Barañao, J.L. (2002) . Paradoxical effects of tumour necrosis factor-α on rat granulosa cell DNA synthesis. Reproduction, Fertility and Development, 14(3-4), 133-139.
http://dx.doi.org/10.1071/RD01103
---------- CHICAGO ----------
Lanuza, G.M., Saragüeta, P.E., Bussmann, U.A., Barañao, J.L. "Paradoxical effects of tumour necrosis factor-α on rat granulosa cell DNA synthesis" . Reproduction, Fertility and Development 14, no. 3-4 (2002) : 133-139.
http://dx.doi.org/10.1071/RD01103
---------- MLA ----------
Lanuza, G.M., Saragüeta, P.E., Bussmann, U.A., Barañao, J.L. "Paradoxical effects of tumour necrosis factor-α on rat granulosa cell DNA synthesis" . Reproduction, Fertility and Development, vol. 14, no. 3-4, 2002, pp. 133-139.
http://dx.doi.org/10.1071/RD01103
---------- VANCOUVER ----------
Lanuza, G.M., Saragüeta, P.E., Bussmann, U.A., Barañao, J.L. Paradoxical effects of tumour necrosis factor-α on rat granulosa cell DNA synthesis. Reprod. Fertil. Dev. 2002;14(3-4):133-139.
http://dx.doi.org/10.1071/RD01103