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Abstract:

Whole cells of the yeast Rhodotorula minuta were used in the biotransformation of dialkyl esters of 2-oxoglutaric acid. Almost 100% of conversion with 97-98% of enantiomeric excess of the (S) form of 2-hydroxydiesters was obtained through an enantioselective reduction of dimethyl and diethyl 2-oxoglutarate. When longer alkoxy chain 2-oxoglutarates were used as substrates, the corresponding 4-hydroxybutyric esters were obtained, suggesting a combination process including hydrolysis, decarboxylation and reduction. The cells showed a remarkable high productivity: high conversion and enantiomeric excess were obtained at 2 g wet weight mmol-1 substrate.

Registro:

Documento: Artículo
Título:An efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by Rhodotorula minuta whole cells
Autor:Rustoy, E.; Cerrutti, P.; Galvagno, M.; Baldessari, A.
Filiación:Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Ingeniería Química, Facultad de Ingeniería, Universidad de Buenos Aires, Buenos Aires, Argentina
IIB-Intech-CONICET-UNSAM, Buenos Aires, San Martín, Argentina
Palabras clave:Biotransformation; Dialkyl 2-oxoglutaric acid esters; Rhodotorula minuta; Cells; Enantiomers; Enantioselectivity; Yeast; Biotransformation; Dialkyl esters; Oxoglutaric acid; Esters; 2 oxoglutaric acid; 4 hydroxybutyric acid; article; biocatalyst; biotransformation; decarboxylation; enantiomer; enantioselectivity; hydrolysis; nonhuman; reduction; Rhodotorula; Rhodotorula minuta; Rhodotorula minuta
Año:2008
Volumen:26
Número:3
Página de inicio:204
Página de fin:209
DOI: http://dx.doi.org/10.1080/10242420701661172
Título revista:Biocatalysis and Biotransformation
Título revista abreviado:Biocatal. Biotransform.
ISSN:10242422
CODEN:BOBOE
CAS:2 oxoglutaric acid, 328-50-7; 4 hydroxybutyric acid, 591-81-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10242422_v26_n3_p204_Rustoy

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Citas:

---------- APA ----------
Rustoy, E., Cerrutti, P., Galvagno, M. & Baldessari, A. (2008) . An efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by Rhodotorula minuta whole cells. Biocatalysis and Biotransformation, 26(3), 204-209.
http://dx.doi.org/10.1080/10242420701661172
---------- CHICAGO ----------
Rustoy, E., Cerrutti, P., Galvagno, M., Baldessari, A. "An efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by Rhodotorula minuta whole cells" . Biocatalysis and Biotransformation 26, no. 3 (2008) : 204-209.
http://dx.doi.org/10.1080/10242420701661172
---------- MLA ----------
Rustoy, E., Cerrutti, P., Galvagno, M., Baldessari, A. "An efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by Rhodotorula minuta whole cells" . Biocatalysis and Biotransformation, vol. 26, no. 3, 2008, pp. 204-209.
http://dx.doi.org/10.1080/10242420701661172
---------- VANCOUVER ----------
Rustoy, E., Cerrutti, P., Galvagno, M., Baldessari, A. An efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by Rhodotorula minuta whole cells. Biocatal. Biotransform. 2008;26(3):204-209.
http://dx.doi.org/10.1080/10242420701661172