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Abstract:

Objective: In the present work we studied the H1 and H2 histamine receptor expression and function in HBL- 100 and MCF-10A cells, derived from non-tumorigenic human breast epithelia, and in MCF-10T, the H-ras-transfected MCF-10A counterpart. The signal transduction pathways associated with these receptors, and the expression of proto-oncogenes c-los, c-myc and c-jun at the mRNA and protein levels, were examined. Results: Saturation analysis using intact cells, showed two binding sites for [3H]tiotidine and [3H]mepyramine. Pretreatment of purified membrane with guanosine 5′-y thiotriphosphate resulted in the loss of the low affinity component for [3H]tiotidine binding, and of the high affinity component for [3H]mepyramine. In both cases, there was no modification in the total number of sites for both ligands. Neither H1 nor H2 agonists stimulated cyclic AMP production, though this pathway is functional in these cells. On the other hand, both H1 and H2 agonists enhanced phosphoinositide turnover in a dose-dependent fashion, and this induction is pertussis toxin-insensitive. H1 and H2 agonists did not influence the expression of c-myc or c-fos mRNA, nor their encoded proteins. Conclusions: These results indicate that the three cell lines examined showed functional H1 and H2 histamine receptors, which are involved in the metabolic turnover of inositol phosphates but are ineffective in the modulation of the cyclic AMP response. The fact that H2 receptors have lost their ability to stimulate cyclic AMP production would imply the loss of a regulatory mechanism of cell growth.

Registro:

Documento: Artículo
Título:H1 and H2 histamine receptors mediate the production of inositol phosphates but not cAMP in human breast epithelial cells
Autor:Davio, C.; Mladovan, A.; Lemos, B.; Monczor, F.; Shayo, C.; Rivera, E.; Baldi, A.
Filiación:Facultad de Farmacia y Bioquímica, Laboratorio de Radioisotopos, Universidad de Buenos Aires, Junin 956 PB, (1113), Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental, Vta. de Obligado 2490, (1428), Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina
Facultad de Ciencias Exactas y Naturales, UBA, Argentina
Palabras clave:C-los; C-myc; Human breast epithelial cells; Signal transduction; cyclic AMP; histamine H1 receptor; histamine H2 receptor; inositol phosphate; mepyramine; pertussis toxin; phosphatidylinositide; tiotidine; cyclic AMP; guanine nucleotide binding protein; histamine H1 receptor; histamine H2 receptor; inositol phosphate; article; binding site; breast epithelium; cell growth; controlled study; gene expression; human; human cell; oncogene c fos; oncogene c myc; proto oncogene; regulatory mechanism; signal transduction; biosynthesis; breast; cell culture; epithelium cell; female; metabolism; oncogene; oncogene myc; physiology; signal transduction; Breast; Cells, Cultured; Cyclic AMP; Epithelial Cells; Female; Genes, fos; Genes, myc; GTP-Binding Proteins; Humans; Inositol Phosphates; Receptors, Histamine H1; Receptors, Histamine H2; Signal Transduction
Año:2002
Volumen:51
Número:1
Página de inicio:1
Página de fin:7
DOI: http://dx.doi.org/10.1007/PL00000276
Título revista:Inflammation Research
Título revista abreviado:Inflamm. Res.
ISSN:10233830
CODEN:INREF
CAS:cyclic AMP, 60-92-4; inositol phosphate, 15421-51-9; mepyramine, 6036-95-9, 91-84-9; pertussis toxin, 70323-44-3; tiotidine, 69014-14-8; Cyclic AMP, 60-92-4; GTP-Binding Proteins, EC 3.6.1.-; Inositol Phosphates; Receptors, Histamine H1; Receptors, Histamine H2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10233830_v51_n1_p1_Davio

Referencias:

  • Hill, S.J., Ganellin, C.R., Timmerman, N.H., Schwartz, J.C., Shankeley, N.P., Young, J.M., International Union of Pharmacology XIII. Clasiffication of histamine receptors (1997) Pharmacological Rev, 49, pp. 253-278
  • Smit, M.J., Timmerman, H., Hijzelendoorn, J.C., Fukui, H., Leurs, R., Regulation of the human histamine H1 receptor stably expressed in Chinese hamster ovary cells (1996) Br J Pharmacol, 117 (6), pp. 1071-1080
  • Traiffort, E., Vizuete, M., Tardivel-Lacombe, J., Souil, E., Schwartz, J.C., Ruat, M., The guinea pig histamine H2 receptor: Gene cloning, tissue expression and chromosomal localization of its human counterpart (1995) Biochem Biophys Res Commun, 211, pp. 570-577
  • Savarese, T.M., Fraser, C.M., In vitro mutagenesis and the search for structure-function relationships among G protein-coupled receptors (1992) Biochem J, 283, pp. 1-19
  • Del Valle, J., Tsunoda, Y., Williams, J., Yamada, T., Regulation of [Ca2+]i by secretagogue stimulation of canine gastric parietal cells (1992) Am J Physiol, 262, pp. G420-G426
  • Mitsuhashi, M., Mitsuhashi, T., Payan, D., Multiple signaling pathways of histamine H2-receptors (1989) J Biol Chem, 264, pp. 18356-18362
  • Kuhn, B., Schmid, A., Harteneck, C., Gudermarm, T., Schultz, G., G proteins of the G(q) family couple the H2 histamine receptor to phospholipase C (1996) Mol Endocrinol, 10, pp. 1697-1707
  • Davio, C., Cricco, G., Bergoc, R., Rivera, E., H1 and H2 histamine receptors in experimental carcinomas with an atypical coupling to signal transducers (1995) Biochem Pharmacol, 49, pp. 91-96
  • Cricco, G., Davio, C., Bergoc, R., Rivera, E., Inhibition of tumor growth induced by histamine: In vivo and in vitro studies (1993) Agents Actions, 38, pp. C175-C177
  • Engel, N., Cricco, G., Davio, C., Martin, G., Croci, M., Fitzsimons, C., Histamine regulates the expression of histidine decarboxylase in nmu-induced tumors in rats (1996) Inflamm Res, 45 (SUPPL. 1), pp. S63-S64
  • Rivera, E., Davio, C., Cricco, G., Bergoc, R., Histamine regulation of tumour growth. Role of H1 and H2 receptors (1993) Histamine in normal and cancer cell proliferation, 89, pp. 299-317. , Garcia-Caballero et al. editors. Adv in Biosciences, Pergamon Press, Oxford
  • Cricco, G., Davio, C., Martin, G., Engel, N., Fitzsimons, C., Bergoc, R., Histamine as an autocrine growth factor in experimental mammary carcinomas (1994) Agents Actions, 43, pp. 17-20
  • Davio, C., Cricco, G., Andrade, N., Bergoc, R., Rivera, E., H1 and H2 histamine receptors in human mammary carcinomas (1993) Agents Actions, 38, pp. 172-174
  • Lemos, B., Davio, C., Gass, H., Gonzalez, P., Cricco, G., Martin, G., Histamine receptors in human mammary gland, different benign lesions and mammary carcinomas (1995) Inflamm Res, 44 (SUPPL. 1), pp. S68-S69
  • Russo, J., Tait, L., Russo, I.H., Morphological expression of cell transformation induced by c-Ha-ras oncogene in human breast epithelial cells (1991) J Cell Sci, 99, pp. 453-463
  • Soule, H.D., Maloney, T.M., Wolman, S.R., Peterson, W.D., Brenz, R., McGrath, C.M., Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10 (1990) Cancer Res, 50, pp. 6075-6086
  • Caron de Fromentel, C., Nardeux, P.C., Soussi, T., Lavialle, C., Estrade, S., Carloni, G., Epithelial HBL-100 cell line derived from milk of an apparently healthy woman harbors SV40 genetic information (1985) Exp Cell Res, pp. 83-94
  • Munson, P.J., Rodbard, D., LIGAND: A versatile computerized approach for characterization of ligand-binding system (1980) Anal Biochem, 107, pp. 220-239
  • Freter, C.E., Lippman, M.E., Cheville, A., Zinn, S., Gelmann, E., Alteration in phosphoinositide metabolism associated with 17β-estradiol and growth factor treatment of MCF-7 breast cancer cells (1988) Molecular Endocrinology, 2, pp. 159-165
  • Chomczynski, P., Sacchi, N., Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction (1987) Anal Biochem, 162, pp. 156-159
  • Shayo, C., Davio, C., Brodsky, A., Mladovan, A., Lemos Legnazzi, B., Rivera, E., Histamine modulates the expression of c-fos through cyclic AMP production via the H2 receptor in the human promonocytic cell line U-937 (1997) Mol Pharmacol, 51, pp. 983-990
  • Fitzsimons, C., Molinari, B., Duran, H., Palmieri, M., Davio, C., Cricco, G., Atypical association of H1 and H2 histamine receptors with signal transduction pathways during multistage mouse skin carcinogenesis (1997) Inflamm Res, 46, pp. 292-298
  • Leurs, R., Smit, M.J., Timmerman, H., Molecular pharmacological aspects of histamine receptors (1995) Pharmacol Ther, 66, pp. 413-462
  • Mitsuhaschi, M., Payan, D., Functional diversity of histamine and histamine receptors (1992) J Invest Dermatol, 98. , 8S-11S
  • Gajtkowski, G.A., Norris, D.B., Rising, T.J., Wood, T.P., Specific binding of [3H]tiotidine to histamine H2 receptors in guinea-pig cerebral cortex (1983) Nature, 304, pp. 65-67
  • Davio, C., Baldi, A., Shayo, C., Brodsky, A., Cricco, G., Bergoc, R., H1 and H2 histamine receptors in histiocytic lymphoma cell line U-937 (1995) Inflamm Res, 44, pp. 72-73
  • Fukushima, Y., Oka, Y., Katagiri, H., Saitoh, T., Asano, T., Ishihara, H., Desensitization of canine histamine H2 receptor expressed in Chinese hamster ovary cells (1993) Biochem Biophys Res Commun, 190, pp. 1149-1155
  • Wisden, W., Parker, E.M., Mahle, C.D., Grisel, D.A., Nowak, H.P., Yocca, F.K., Cloning and characterization of the rat 5-HT5B receptor. Evidence that the 5-HT5B receptor couples to a G protein in mammalian cell membranes (1993) FEBS Lett, 333, pp. 25-30
  • Milligan, G., Bond, R.A., Inverse agonism and the regulation of receptor number (1997) Trends Pharmacol Sci, 18, pp. 468-474
  • Samama, P., Cotecchia, S., Costa, T., Lefkowitz, R., A mutation induced activated state of the β2-adrenergic receptor. Extending the ternary complex model (1993) J Biol Chem, 268, pp. 4625-4636
  • Birnbaumer, L., Swartz, T., Abramowitz, J., Mintz, P., Iyengar, R., Transient and steady state kinetics of guanyl nucleotides with the adenylyl cyclase system from rat liver plasma membranes. Interpretations in terms of a simple two-state model (1980) J Biol Chem, 255, pp. 3542-3551
  • Weiss, J., Morgan, P., Lutz, M., Kenakin, T., The cubic ternary complex receptor-occupancy model I. Model description (1996) J Theor Biol, 178, pp. 151-167
  • Kenakin, T., Morgan, P., Lutz, M., On the importance of the "antagonist assumption" to how receptors express themselves (1995) Biochem Pharmacol, 50, pp. 17-26
  • Kenakin, T., Agonist-receptor efficacy I Mechanisms of efficacy and receptor promiscuity (1995) Trends Pharmacol Sci, 16 (6), pp. 188-192
  • Kenakin, T., Differences between natural and recombinant G protein-coupled receptor systems with varying receptor/G protein stoichiometry (1997) Trends Pharmacol Sci, 18, pp. 456-464
  • Orange, P., Heath, P., Wright, S., Pearson, R., Allelic variation of the human histamine H2 receptor gene (1996) Neuroreport, 7, pp. 1293-1296
  • Park, M., Oncogenes: Genetic abnormalities of cell growth (1995) The metabolic and molecular basis of inherited disease, pp. 589-611. , Scriver CR et al. editors. McGraw-Hill Inc., New York
  • Davio, C., Cricco, G., Martin, G., Fitzsimons, C., Bergoc, R., Rivera, E., Role of histamine on growth and differentiation of the rat mammary gland (1994) Agents Actions, 41, pp. C115-C117
  • Cho-Chung, Y.S., Clair, T., Tortora, G., Yokozaki, H., Role of site-selective cAMP analogs in the control and reversal of malignancy (1991) Pharmacol Ther, 50, pp. 1-33
  • Hartwell, L., cAMPing out (1994) Nature, 371, p. 286
  • Houge, G., Cho-Chung, Y.S., Doskeland, S.O., Differential expression of cAMP-kinase is correlated with growth in rat mammary carcinomas and uterus (1992) Br J Cancer, 66, pp. 1022-1029
  • Wu, J., Dent, P., Jelinek, T., Wolfman, A., Weber, M.J., Sturgill, T.W., Inhibition of the EGF-activated MAP-kinase signaling by adenosine 3′,5′-monophosphate (1993) Science, 262, pp. 1065-1072
  • Cook, S.J., McCormick, F., Inhibition by cAMP of ras-dependent activation of raf (1993) Science, 262, pp. 1069-1072

Citas:

---------- APA ----------
Davio, C., Mladovan, A., Lemos, B., Monczor, F., Shayo, C., Rivera, E. & Baldi, A. (2002) . H1 and H2 histamine receptors mediate the production of inositol phosphates but not cAMP in human breast epithelial cells. Inflammation Research, 51(1), 1-7.
http://dx.doi.org/10.1007/PL00000276
---------- CHICAGO ----------
Davio, C., Mladovan, A., Lemos, B., Monczor, F., Shayo, C., Rivera, E., et al. "H1 and H2 histamine receptors mediate the production of inositol phosphates but not cAMP in human breast epithelial cells" . Inflammation Research 51, no. 1 (2002) : 1-7.
http://dx.doi.org/10.1007/PL00000276
---------- MLA ----------
Davio, C., Mladovan, A., Lemos, B., Monczor, F., Shayo, C., Rivera, E., et al. "H1 and H2 histamine receptors mediate the production of inositol phosphates but not cAMP in human breast epithelial cells" . Inflammation Research, vol. 51, no. 1, 2002, pp. 1-7.
http://dx.doi.org/10.1007/PL00000276
---------- VANCOUVER ----------
Davio, C., Mladovan, A., Lemos, B., Monczor, F., Shayo, C., Rivera, E., et al. H1 and H2 histamine receptors mediate the production of inositol phosphates but not cAMP in human breast epithelial cells. Inflamm. Res. 2002;51(1):1-7.
http://dx.doi.org/10.1007/PL00000276