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Abstract:

Objective: In the present work we studied the association of histamine receptors with second messengers during multistage carcinogenesis in Sencar mice skin. Methods: 96 Sencar female mouse, divided into six groups were used. Tumors appeared only in the 7,12-dimethyl-benz[a]anthracene-initiated and 12-O-tetradecanoylphorbol-13-acetate-promoted group. Control groups received only TPA, or acetone or no treatment at all. Periodically during the promotion period, cAMP and inositol phosphate production were measured after stimulation with H1 or H2 agonists in samples from all groups. Results: In non-treated skin, H1 receptors were coupled to phosphatidylinositol hydrolysis and H2 receptors mediated cAMP production. Conversely, in tumors H2 receptors were associated with phosphatidylinositol hydrolysis and H1 mediated a rise in cAMP levels. The skin among tumors and the skin from all control groups maintained the same coupling as non-treated skin. An increase in mast cell number, with a homogeneous subepithelial distribution and marked phenotypic changes, was also observed in promoted skin. Conclusions: These findings indicate an atypical association of histamine receptors with second messengers that could be a critical feature for the postulated action of histamine in tumor growth.

Registro:

Documento: Artículo
Título:Atypical association of H1 and H2 histamine receptors with signal transduction pathways during multistage mouse skin carcinogenesis
Autor:Fitzsimons, C.; Molinari, B.; Duran, H.; Palmieri, M.; Davio, C.; Cricco, G.; Bergoc, R.; Rivera, E.
Filiación:Laboratorio de Radioisótopos, Fac. de Farmacia/Bioquímiea, Universidad de Buenos Aires, Junin 956, 1113 Buenos Aires, Argentina
Departamento de Radiobiología, Comn. Nac. de Ener. Alómica, Laboratorio Tandar, Av. Gral Paz y Constituyentes, 1464 Pcia. de Buenos Aires, Argentina
Departamento de Biología, Fac. de Ciencias Exactas/Naturales, Ciudad Universitaria, Buenos Aires, Argentina
Palabras clave:cAMP; Histamine receptors; Inositol phosphates; Mouse skin carcinogenesis; 7,12 dimethylbenz[a]anthracene; acetone; cyclic amp; histamine; histamine agonist; histamine h1 receptor; histamine h2 receptor; histamine receptor; inositol phosphate; phorbol 13 acetate 12 myristate; phosphatidylinositol; cimetidine; cyclic AMP; drug derivative; histamine H1 receptor; histamine H1 receptor antagonist; histamine H2 receptor; histamine H2 receptor antagonist; mepyramine; phosphatidylinositol; tiotidine; animal cell; animal experiment; animal model; article; cellular distribution; controlled study; drug hydrolysis; female; mast cell; mouse; nonhuman; phenotype; second messenger; signal transduction; skin carcinogenesis; tumor growth; animal; cell count; chemically induced disorder; hydrolysis; metabolism; pathology; pathophysiology; physiology; skin tumor; 9,10-Dimethyl-1,2-benzanthracene; Animals; Cell Count; Cimetidine; Cyclic AMP; Female; Histamine H1 Antagonists; Histamine H2 Antagonists; Hydrolysis; Mast Cells; Mice; Phosphatidylinositols; Pyrilamine; Receptors, Histamine H1; Receptors, Histamine H2; Second Messenger Systems; Signal Transduction; Skin Neoplasms; Tetradecanoylphorbol Acetate
Año:1997
Volumen:46
Número:8
Página de inicio:292
Página de fin:298
DOI: http://dx.doi.org/10.1007/s000110050190
Título revista:Inflammation Research
Título revista abreviado:INFLAMM. RES.
ISSN:10233830
CODEN:INREF
CAS:7,12 dimethylbenz[a]anthracene, 57-97-6; acetone, 67-64-1; cyclic AMP, 60-92-4; histamine, 51-45-6, 56-92-8, 93443-21-1; inositol phosphate, 15421-51-9; phorbol 13 acetate 12 myristate, 16561-29-8; 9,10-Dimethyl-1,2-benzanthracene, 57-97-6; Cimetidine, 51481-61-9; Cyclic AMP, 60-92-4; Histamine H1 Antagonists; Histamine H2 Antagonists; Phosphatidylinositols; Pyrilamine, 91-84-9; Receptors, Histamine H1; Receptors, Histamine H2; Tetradecanoylphorbol Acetate, 16561-29-8; tiotidine, 69014-14-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10233830_v46_n8_p292_Fitzsimons

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Citas:

---------- APA ----------
Fitzsimons, C., Molinari, B., Duran, H., Palmieri, M., Davio, C., Cricco, G., Bergoc, R.,..., Rivera, E. (1997) . Atypical association of H1 and H2 histamine receptors with signal transduction pathways during multistage mouse skin carcinogenesis. Inflammation Research, 46(8), 292-298.
http://dx.doi.org/10.1007/s000110050190
---------- CHICAGO ----------
Fitzsimons, C., Molinari, B., Duran, H., Palmieri, M., Davio, C., Cricco, G., et al. "Atypical association of H1 and H2 histamine receptors with signal transduction pathways during multistage mouse skin carcinogenesis" . Inflammation Research 46, no. 8 (1997) : 292-298.
http://dx.doi.org/10.1007/s000110050190
---------- MLA ----------
Fitzsimons, C., Molinari, B., Duran, H., Palmieri, M., Davio, C., Cricco, G., et al. "Atypical association of H1 and H2 histamine receptors with signal transduction pathways during multistage mouse skin carcinogenesis" . Inflammation Research, vol. 46, no. 8, 1997, pp. 292-298.
http://dx.doi.org/10.1007/s000110050190
---------- VANCOUVER ----------
Fitzsimons, C., Molinari, B., Duran, H., Palmieri, M., Davio, C., Cricco, G., et al. Atypical association of H1 and H2 histamine receptors with signal transduction pathways during multistage mouse skin carcinogenesis. INFLAMM. RES. 1997;46(8):292-298.
http://dx.doi.org/10.1007/s000110050190