Artículo
Leasen, S.; Hodak, J.H.; Srisala, J.; Srikhirin, T.; Sritunyalucksana, K.; Veerasai, W.; Dangtip, S. "Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self- assembled on tin-doped indium oxide film with electrochemical measurement" (2013) 2013 International Conference on Applied Physics and Material Applications, ICAPMA2013. 770:402-408
Estamos trabajando para incorporar este artículo al repositorio
Consulte la política de Acceso Abierto del editor
Abstract:
Self-assembled monolayers (SAM) of 3-mercaptopropionic acid (MPA) were applied on tin-doped indium oxide (ITO) surfaces and used as a working electrode for sensing DNA hybridization. The concentration of probe single stranded DNA (ssDNA), complemented with target DNA, was optimized for the highest yield immobilization on MPA/ITO platform. The ssDNA/MPA/ITO was allowed to hybridize to target DNA prepared from PCR amplification that first tested by the synthesized complementary sequences. Both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed for investigating probe ssDNA immobilization and target DNA hybridization. For fast and low concentration detecting purposes, methylene blue (MB) coupled with differential pulse voltammetry (DPV) was used for detecting the target DNA hybridization events. © (2013) Trans Tech Publications, Switzerland.
Registro:
| Documento: | Artículo |
| Título: | Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self- assembled on tin-doped indium oxide film with electrochemical measurement |
| Autor: | Leasen, S.; Hodak, J.H.; Srisala, J.; Srikhirin, T.; Sritunyalucksana, K.; Veerasai, W.; Dangtip, S. |
| Ciudad: | Petchaburi |
| Filiación: | Department of Physics, Mahidol University, Bangkok, Thailand INQUIMAE-DQIAyQF, Universidad de Buenos Aires, Buenos Aires 1428, Argentina Centex Shrimp, Mahidol University, Bangkok, Thailand NANOTEC CoE at Mahidol University, Mahidol University, Bangkok, Thailand IFEC-MU, Chemistry Department, CoE for Innovation in Chemistry Mahidol University, Bangkok, Thailand National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, 12120, Thailand |
| Palabras clave: | 3-mercaptopropionic acid; Differential pulse voltammetry; Hybridization; Methylene blue; Tin-doped indium oxide; 3-mercaptopropionic acid; Differential pulse voltammetry; Hybridization; Methylene Blue; Tin doped indium oxide; Aromatic compounds; Cyclic voltammetry; DNA; DNA sequences; Organic polymers; Oxide films; Probes; Self assembled monolayers; Tin; Polymerase chain reaction |
| Año: | 2013 |
| Volumen: | 770 |
| Página de inicio: | 402 |
| Página de fin: | 408 |
| DOI: | http://dx.doi.org/10.4028/www.scientific.net/AMR.770.402 |
| Título revista: | 2013 International Conference on Applied Physics and Material Applications, ICAPMA2013 |
| Título revista abreviado: | Adv. Mater. Res. |
| ISSN: | 10226680 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10226680_v770_n_p402_Leasen |
Referencias:
- Lin, J., Qu, W., Zhang, S., Disposable biosensor based on enzyme immobilized on Auchitosan- modified indium tin oxide electrode with flow injection amperometric analysis (2007) Analytical Biochemistry, 360, pp. 288-293
- Drummond, T.G., Hill, M.G., Barton, J.K., Electrochemical DNA sensors (2003) Nature Biotechnology, 21, pp. 1192-1199
- Kelly, S.O., Barton, J.K., Electrochemistry of Methylene Blue bound to a DNA-Modified Electrode (1997) Bioconjugate Chem, 8, pp. 31-37
- Cho, S.B., Hong, J.S., Pak, Y.K., Pak, J.J., Electrochemical detection of hybridized DNA using reduction of methylene blue (2001) 23rd Annual EMBS International Conference, 25-28, pp. 2937-2940. , Istanbul, Turkey, October
- Takagi, M., Threading intercalation to double-stranded DNA and the application to DNA sensing. Electrochemical array technique (2001) Pure Appl. Chem, 73, pp. 1573-1577
- Black, M., Cooper, J., McGinn, P., Scanning electrochemical microscope characterization of thin film combinatorial libraries for fuel cell electrode applications (2005) Meas. Sci. Technol, 16, pp. 174-182
- Kirchner, C.N., Szunerits, S., Wittstock, G., Scanning Electrochemical Microscopy (SECM) Based Detection Oligonucleotide Hybridization and Simultaneous Determination of the Surface Concentration of Immobilized Oligonucleotides on Gold (2007) Electroanalysis, 19, pp. 1258-1267
- Lee, T.M., Li, L.L., Hsing, I.M., Enhanced Electrochemical Detection of DNA Hybridization Based on Electrode-Surface Modification (2003) Langmuir, 19, pp. 4338-4343
- Gardner, T.J., Frisbie, C.D., Wrighton, M.S., Systems for Orthogonal Self-Assembly of Electroactive Monolayers on Au and ITO: An Approach to Molecular Electronics (1995) J. Am. Chem. Soc, 117, pp. 6927-6933
- Compton, R.G., Banks, C.E., (2011) Understanding Voltammetry, , Imperial College Press, London
- Pan, D., Zuo, X., Wan, Y., Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene blue (2007) Sensors, 7, pp. 2671-2680
- Nasef, H., Beni, V., Methylene blue as an electrochemical indicator for DF508 cystic fibrosis mutation detection (2010) Anal. Bioanal. Chem, 396, pp. 1423-1432
- Kara, P., Kerman, K., Electrochemical genosensor for detection of interaction between methylene blue and DNA (2002) Electrochemistry Communications, 4, pp. 705-709
Citas:
---------- APA ----------
Leasen, S., Hodak, J.H., Srisala, J., Srikhirin, T., Sritunyalucksana, K., Veerasai, W. & Dangtip, S. (2013) . Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self- assembled on tin-doped indium oxide film with electrochemical measurement. 2013 International Conference on Applied Physics and Material Applications, ICAPMA2013, 770, 402-408.http://dx.doi.org/10.4028/www.scientific.net/AMR.770.402
---------- CHICAGO ----------
Leasen, S., Hodak, J.H., Srisala, J., Srikhirin, T., Sritunyalucksana, K., Veerasai, W., et al. "Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self- assembled on tin-doped indium oxide film with electrochemical measurement" . 2013 International Conference on Applied Physics and Material Applications, ICAPMA2013 770 (2013) : 402-408.http://dx.doi.org/10.4028/www.scientific.net/AMR.770.402
---------- MLA ----------
Leasen, S., Hodak, J.H., Srisala, J., Srikhirin, T., Sritunyalucksana, K., Veerasai, W., et al. "Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self- assembled on tin-doped indium oxide film with electrochemical measurement" . 2013 International Conference on Applied Physics and Material Applications, ICAPMA2013, vol. 770, 2013, pp. 402-408.http://dx.doi.org/10.4028/www.scientific.net/AMR.770.402
---------- VANCOUVER ----------
Leasen, S., Hodak, J.H., Srisala, J., Srikhirin, T., Sritunyalucksana, K., Veerasai, W., et al. Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self- assembled on tin-doped indium oxide film with electrochemical measurement. Adv. Mater. Res. 2013;770:402-408.http://dx.doi.org/10.4028/www.scientific.net/AMR.770.402
