Abstract:
We have previously described the regulation of interleukin-1 receptor antagonist (IL-1ra) protein secretion and expression by IL-1, glucocorticoids and corticotropin-releasing hormone in monocytes in culture. In the present work, we analyze the direct effect of adrenocorticotropic hormone (ACTH) and β-endorphin on the expression and secretion of IL-1ra by human monocytes in culture. ACTH exerted a dose-dependent inhibitory effect on lipopolysaccharide (LPS)-induced IL-1ra production and mRNA expression. Basal IL-1ra levels were not affected by treatment with any ACTH dose. In contrast, on human monocytes, β-endorphin at concentrations as low as 10 pg/ml produced an increase of basal IL-1ra protein secretion and mRNA expression, this effect being reverted by pretreatment with naloxone. No effect of β- endorphin was observed either in IL-1ra mRNA expression or protein secretion when cells were treated with LPS. The different effects of ACTH and β- endorphin could account for their differential contribution to the inflammatory response: while ACTH contributes to the glucocorticoid overall control of the inflammatory response, β-endorphin exerts an inhibitory tone on the resting IL-1 system. Because IL-1ra is essential in setting the level of monocyte and inflammatory response its differential regulation by the HPA axis hormones contributes to regulating the IL-1/inflammatory temporal response.
Registro:
Documento: |
Artículo
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Título: | Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin |
Autor: | Kovalovsky, D.; Pereda, M.P.; Stalla, G.K.; Holsboer, F.; Arzt, E. |
Filiación: | Lab. de Fisiol. y Biol. Molecular, Departamento de Biología, Universidad de Buenos Aires, Argentina Max Planck Institute of Psychiatry, Clinical Institute, Munich, Germany Lab. de Fisiol. y Biol. Molecular, Universidad de Buenos Aires, Pabellón II, 1428 Buenos Aires, Argentina
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Palabras clave: | β-Endorphin; Adrenocorticotropic hormone; Glucocorticoids; Interleukin- 1; Interleukin-1 receptor antagonist; beta endorphin; corticotropin; interleukin 1 receptor; proopiomelanocortin; article; cytokine production; cytokine release; dose response; hormonal regulation; hormone action; human; human cell; hypothalamus hypophysis system; immunoregulation; monocyte; priority journal; Adrenocorticotropic Hormone; beta-Endorphin; Blotting, Northern; Cells, Cultured; Humans; Interleukin 1 Receptor Antagonist Protein; Monocytes; Pro-Opiomelanocortin; Receptors, Interleukin-1; Sialoglycoproteins |
Año: | 1999
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Volumen: | 6
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Número: | 5
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Página de inicio: | 367
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Página de fin: | 372
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DOI: |
http://dx.doi.org/10.1159/000026397 |
Título revista: | NeuroImmunoModulation
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Título revista abreviado: | NeuroImmunomodulation
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ISSN: | 10217401
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CODEN: | NROIE
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CAS: | Adrenocorticotropic Hormone, 9002-60-2; beta-Endorphin, 60617-12-1; IL1RN protein, human; Interleukin 1 Receptor Antagonist Protein; Pro-Opiomelanocortin, 66796-54-1; Receptors, Interleukin-1; Sialoglycoproteins
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10217401_v6_n5_p367_Kovalovsky |
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Citas:
---------- APA ----------
Kovalovsky, D., Pereda, M.P., Stalla, G.K., Holsboer, F. & Arzt, E.
(1999)
. Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin. NeuroImmunoModulation, 6(5), 367-372.
http://dx.doi.org/10.1159/000026397---------- CHICAGO ----------
Kovalovsky, D., Pereda, M.P., Stalla, G.K., Holsboer, F., Arzt, E.
"Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin"
. NeuroImmunoModulation 6, no. 5
(1999) : 367-372.
http://dx.doi.org/10.1159/000026397---------- MLA ----------
Kovalovsky, D., Pereda, M.P., Stalla, G.K., Holsboer, F., Arzt, E.
"Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin"
. NeuroImmunoModulation, vol. 6, no. 5, 1999, pp. 367-372.
http://dx.doi.org/10.1159/000026397---------- VANCOUVER ----------
Kovalovsky, D., Pereda, M.P., Stalla, G.K., Holsboer, F., Arzt, E. Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin. NeuroImmunomodulation. 1999;6(5):367-372.
http://dx.doi.org/10.1159/000026397