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Abstract:

Interleukin-l (IL-1) is an important mediator in the mechanisms underlying the immune and inflammatory responses. It has pleiotropic effects in host defense and, when present in high concentrations, participates in the development of pathological processes. IL-1 is the most potent cytokine in the activation of the hypothalamic-pituitary-adrenal axis during infection and therefore leads to a glucocorticoid increase. Glucocorticoids in a feedback loop inhibit the production of IL-1 induced by endotoxin. IL-1 also induces its own synthesis. In this report, we examine the role of glucocorticoids in the regulation of IL-1 autoregulatory induction in human monocytes at the level of IL-1 protein production and mRNA accumulation. Using recombinant IL1 receptor antagonist we established that endogenously produced IL-1 affects induction of IL-1βprotein by lipopolysaccharide (LPS) at the level of mRNA expression. The inhibition of LPS-stimulated IL-1βproduction and mRNA expression by glucocorticoids (dexamethasone and Cortisol) reaches the same level with glucocorticoids alone or in combination with rIL-1ra. IL-1βmRNA induced by exogenously added IL-1βwas also inhibited by glucocorticoids. These results indicate that glucocorticoids inhibit the autoregulatory loop of IL-1 in LPS-stimulated monocytes and constitute a mechanism for controlling IL-1 feedback stimulation. © 1996 S. Karger AG, Basel.

Registro:

Documento: Artículo
Título:Glucocorticoids Inhibit the Autoregulatory Induction of lnterleukin-1 in Monocytes after Endotoxin Stimulation
Autor:Pereda, M.P.; Castro, C.P.; Costas, M.; Nahmod, V.E.; Stalla, G.K.; Holsboer, F.; Arzt, E.
Filiación:Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Buenos Aires, Argentina
Max Planck Institute of Psychiatry, Clinical Institute, Munich, Germany
Departamento de Biologia, FCEN, Universidad de Buenos Aires, Argentina
Palabras clave:Endotoxin; Glucocorticoids; Interleukin-l; Monocytes; dexamethasone; glucocorticoid; hydrocortisone; interleukin 1 receptor blocking agent; interleukin 1beta; lipopolysaccharide; messenger rna; mifepristone; adult; article; human; human cell; monocyte; normal human; priority journal; Cells, Cultured; Dose-Response Relationship, Drug; Endotoxins; Glucocorticoids; Homeostasis; Humans; Interleukin-1; Lipopolysaccharides; Monocytes
Año:1996
Volumen:3
Número:4
Página de inicio:227
Página de fin:232
DOI: http://dx.doi.org/10.1159/000097275
Título revista:NeuroImmunoModulation
Título revista abreviado:NeuroImmunomodulation
ISSN:10217401
CAS:dexamethasone, 50-02-2; hydrocortisone, 50-23-7; mifepristone, 84371-65-3; Endotoxins; Glucocorticoids; Interleukin-1; Lipopolysaccharides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10217401_v3_n4_p227_Pereda

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Citas:

---------- APA ----------
Pereda, M.P., Castro, C.P., Costas, M., Nahmod, V.E., Stalla, G.K., Holsboer, F. & Arzt, E. (1996) . Glucocorticoids Inhibit the Autoregulatory Induction of lnterleukin-1 in Monocytes after Endotoxin Stimulation. NeuroImmunoModulation, 3(4), 227-232.
http://dx.doi.org/10.1159/000097275
---------- CHICAGO ----------
Pereda, M.P., Castro, C.P., Costas, M., Nahmod, V.E., Stalla, G.K., Holsboer, F., et al. "Glucocorticoids Inhibit the Autoregulatory Induction of lnterleukin-1 in Monocytes after Endotoxin Stimulation" . NeuroImmunoModulation 3, no. 4 (1996) : 227-232.
http://dx.doi.org/10.1159/000097275
---------- MLA ----------
Pereda, M.P., Castro, C.P., Costas, M., Nahmod, V.E., Stalla, G.K., Holsboer, F., et al. "Glucocorticoids Inhibit the Autoregulatory Induction of lnterleukin-1 in Monocytes after Endotoxin Stimulation" . NeuroImmunoModulation, vol. 3, no. 4, 1996, pp. 227-232.
http://dx.doi.org/10.1159/000097275
---------- VANCOUVER ----------
Pereda, M.P., Castro, C.P., Costas, M., Nahmod, V.E., Stalla, G.K., Holsboer, F., et al. Glucocorticoids Inhibit the Autoregulatory Induction of lnterleukin-1 in Monocytes after Endotoxin Stimulation. NeuroImmunomodulation. 1996;3(4):227-232.
http://dx.doi.org/10.1159/000097275