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Abstract:

Background/Aims: The maternal-fetal interface is a unique immunological site that generates an adequate microenvironment during pregnancy, recognizing and eliminating infections and tolerating the trophoblast/placenta unit. For that purpose, trophoblast cells display several tolerogenic mechanisms to allow fetal survival, such as production of the neuropeptide vasoactive intestinal peptide (VIP). Here we investigated the contribution of VIP to maintain homeostasis at the maternal-placental interface under lipopolysaccharide (LPS) stimulation. Methods: We performed cocultures between trophoblast cells (Swan-71 cell line) and maternal leukocytes obtained from fertile women as an in vitro model of maternal-placental interaction, and we focused on the effects of LPS on the modulation of VIP and their receptors (VPAC1 and VPAC 2). Results: VIP could prevent the upregulation of IL-6, MCP-1, and nitrite production and maintain the production of IL-10 and TGF-β under LPS (10 μg/ml) stimulation after 48 h of coculture. To gain deeper insight into the mechanisms of how VIP could contribute to a tolerogenic microenvironment even in the presence of LPS, we investigated VIP production by maternal leukocytes and observed a significant increase in the frequency of CD4+VIP+ cells after interaction with Swan-71 cells in the presence of LPS. LPS increased VIP and inducible receptor VPAC2 expression directly on trophoblast cells in a dose- and time-dependent manner. Conclusions: The present results suggest that VIP might act as an additional homeostatic mechanism during early stages at the maternal-placental interface to control exacerbated inflammatory responses such as the ones observed in intrauterine infections. © 2013 S. Karger AG, Basel.

Registro:

Documento: Artículo
Título:Contribution of vasoactive intestinal peptide to immune homeostasis in trophoblast-maternal leukocyte interaction under LPS stimulation
Autor:Fraccaroli, L.; Grasso, E.; Hauk, V.; Cortelezzi, M.; Pérez Leirós, C.; Ramhorst, R.
Filiación:Laboratory of Immunopharmacology, School of Sciences, University of Buenos Aires, Int. Guiraldes 2160 Piso 4, Buenos Aires C1428EHA, Argentina
Argentinean Society for Gynecology and Endocrinology Reproduction (SAEGRE), Buenos Aires, Argentina
Palabras clave:Early pregnancy; Human implantation; Tolerance and pregnancy; Vasoactive intestinal peptide; interleukin 10; interleukin 6; lipopolysaccharide; monocyte chemotactic protein 1; nitrite; transforming growth factor beta; vasoactive intestinal polypeptide; vasoactive intestinal polypeptide receptor 1; vasoactive intestinal polypeptide receptor 2; CD14 antigen; CD19 antigen; lipopolysaccharide; vasoactive intestinal polypeptide; article; cell interaction; coculture; controlled study; cytokine production; female; human; human cell; immunomodulation; immunostimulation; in vitro study; leukocyte; microenvironment; mother; peripheral blood mononuclear cell; priority journal; protein expression; protein function; reverse transcription polymerase chain reaction; trophoblast; upregulation; Western blotting; Article; B lymphocyte; CD4+ T lymphocyte; cell line; flow cytometry; homeostasis; immune homeostasis; inflammation; swan 71 cell line; trophoblast; trophoblast maternal leukocyte interaction; CD4-Positive T-Lymphocytes; Coculture Techniques; Cytokines; Dose-Response Relationship, Drug; Female; Flow Cytometry; Gene Expression Regulation; Homeostasis; Humans; Leukocytes; Lipopolysaccharides; Nitrites; Pregnancy; Receptors, Vasoactive Intestinal Peptide, Type II; Receptors, Vasoactive Intestinal Polypeptide, Type I; Time Factors; Transforming Growth Factor beta; Trophoblasts; Vasoactive Intestinal Peptide
Año:2013
Volumen:21
Número:1
Página de inicio:21
Página de fin:30
DOI: http://dx.doi.org/10.1159/000355039
Título revista:NeuroImmunoModulation
Título revista abreviado:NeuroImmunomodulation
ISSN:10217401
CODEN:NROIE
CAS:nitrite, 14797-65-0; vasoactive intestinal polypeptide, 37221-79-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10217401_v21_n1_p21_Fraccaroli

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Citas:

---------- APA ----------
Fraccaroli, L., Grasso, E., Hauk, V., Cortelezzi, M., Pérez Leirós, C. & Ramhorst, R. (2013) . Contribution of vasoactive intestinal peptide to immune homeostasis in trophoblast-maternal leukocyte interaction under LPS stimulation. NeuroImmunoModulation, 21(1), 21-30.
http://dx.doi.org/10.1159/000355039
---------- CHICAGO ----------
Fraccaroli, L., Grasso, E., Hauk, V., Cortelezzi, M., Pérez Leirós, C., Ramhorst, R. "Contribution of vasoactive intestinal peptide to immune homeostasis in trophoblast-maternal leukocyte interaction under LPS stimulation" . NeuroImmunoModulation 21, no. 1 (2013) : 21-30.
http://dx.doi.org/10.1159/000355039
---------- MLA ----------
Fraccaroli, L., Grasso, E., Hauk, V., Cortelezzi, M., Pérez Leirós, C., Ramhorst, R. "Contribution of vasoactive intestinal peptide to immune homeostasis in trophoblast-maternal leukocyte interaction under LPS stimulation" . NeuroImmunoModulation, vol. 21, no. 1, 2013, pp. 21-30.
http://dx.doi.org/10.1159/000355039
---------- VANCOUVER ----------
Fraccaroli, L., Grasso, E., Hauk, V., Cortelezzi, M., Pérez Leirós, C., Ramhorst, R. Contribution of vasoactive intestinal peptide to immune homeostasis in trophoblast-maternal leukocyte interaction under LPS stimulation. NeuroImmunomodulation. 2013;21(1):21-30.
http://dx.doi.org/10.1159/000355039