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Abstract:

Sjögren's syndrome (SS) is a chronic autoimmune disorder of exocrine glands characterized as an autoimmune exocrinopathy and more specifically as an autoimmune epithelitis. An impaired balance of neuroimmune interactions mediated by vasoactive intestinal peptide (VIP) in the target organ at early stages of disease is explored by means of the nonobese diabetic (NOD) mouse model of SS. We have previously described a reduced salivary secretion and signaling upon VIP stimulation. The effect reflected a differential regulation of the neural isoform of nitric oxide synthase by calcium calmodulin kinase II and occurred prior to the appearance of detectable levels of cytokines in NOD glands. VIP acting on NOD macrophages treated with lipopolysaccharide promoted anti-inflammatory effects by inhibiting nitric oxide synthase induction as well as IL-12 and TNF-α production, while stimulating IL-10. Here we present evidence on the ability of apoptotic acinar cells from submandibular glands of NOD mice to stimulate nitric oxide in both peritoneal and glandular macrophage pools to a similar extent as lipopolysaccharide + IFN-γ. VIP was not effective to prevent nitrite accumulation and modestly increased IL-10 levels in macrophages coincubated with acinar cells. An enhanced nitrite response of NOD glandular macrophages in basal and stimulated conditions compared to peritoneal cells is also shown. Copyright © 2007 S. Karger AG.

Registro:

Documento: Artículo
Título:NOD mice exocrinopathy: Towards a neuroimmune link
Autor:Calafat, M.; Larocca, L.; Roca, V.; Pérez Leirós, C.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, CONICET, Buenos Aires, Argentina
Ciudad Universitaria, Pab. II, 1428 Buenos Aires, Argentina
Palabras clave:Apoptotic acinar cells; Exocrinopathy; Macrophages; Neuroimmune; Nitric oxide; NOD mice; gamma interferon; interleukin 10; lipopolysaccharide; nitric oxide; nitrite; vasoactive intestinal polypeptide; acinar cell; animal cell; animal model; apoptosis; conference paper; controlled study; female; macrophage; mouse; nonhuman; nonobese diabetic mouse; peritoneum macrophage; priority journal; Sjoegren syndrome; stimulation; submandibular gland; Animals; Apoptosis; Cells, Cultured; Disease Models, Animal; Epithelial Cells; Epithelium; Female; Inflammation Mediators; Interferon Type II; Interleukin-10; Lipopolysaccharides; Macrophages; Mice; Mice, Inbred BALB C; Mice, Inbred NOD; Neuroimmunomodulation; Nitric Oxide; Peritoneum; Sjogren's Syndrome; Submandibular Gland; Vasoactive Intestinal Peptide
Año:2007
Volumen:14
Número:3-4
Página de inicio:175
Página de fin:181
DOI: http://dx.doi.org/10.1159/000110643
Título revista:NeuroImmunoModulation
Título revista abreviado:NeuroImmunomodulation
ISSN:10217401
CODEN:NROIE
CAS:gamma interferon, 82115-62-6; nitric oxide, 10102-43-9; nitrite, 14797-65-0; vasoactive intestinal polypeptide, 37221-79-7; Inflammation Mediators; Interferon Type II, 82115-62-6; Interleukin-10, 130068-27-8; Lipopolysaccharides; Nitric Oxide, 10102-43-9; Vasoactive Intestinal Peptide, 37221-79-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10217401_v14_n3-4_p175_Calafat

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Citas:

---------- APA ----------
Calafat, M., Larocca, L., Roca, V. & Pérez Leirós, C. (2007) . NOD mice exocrinopathy: Towards a neuroimmune link. NeuroImmunoModulation, 14(3-4), 175-181.
http://dx.doi.org/10.1159/000110643
---------- CHICAGO ----------
Calafat, M., Larocca, L., Roca, V., Pérez Leirós, C. "NOD mice exocrinopathy: Towards a neuroimmune link" . NeuroImmunoModulation 14, no. 3-4 (2007) : 175-181.
http://dx.doi.org/10.1159/000110643
---------- MLA ----------
Calafat, M., Larocca, L., Roca, V., Pérez Leirós, C. "NOD mice exocrinopathy: Towards a neuroimmune link" . NeuroImmunoModulation, vol. 14, no. 3-4, 2007, pp. 175-181.
http://dx.doi.org/10.1159/000110643
---------- VANCOUVER ----------
Calafat, M., Larocca, L., Roca, V., Pérez Leirós, C. NOD mice exocrinopathy: Towards a neuroimmune link. NeuroImmunomodulation. 2007;14(3-4):175-181.
http://dx.doi.org/10.1159/000110643