Artículo
Abstract:
Mast cells (MC) are frequently increased in neoplasias. Recently, we observed that MC from peritoneal cavity of normal mice (NMC) and one of their mediators (heparin) decreased M3 tumor incidence and tumor cell proliferation in vitro, while MC from peritoneal cavity of tumor-bearing mice (TMC) had no effect. The purpose of this study was to evaluate the differences in morphology and content of sulfated glycosaminoglycans between NMC and TMC. Both were stained with Mayer's haematoxylin-Rubipy [tris (2,2'-bipyridine) ruthenium (II)] sequence, a specific technique to detect sulfated polysaccharides. Image processing and analysis (IPA) confirmed densitometric and microfluorometric studies and revealed several structural characteristics of MC. TMC were partially degranulated with smaller surface and greater perimeter than NMC. Shape factor, which reflects the sphericity grade of the cell (1 = spherical), was three-fold increased in TMC in relation to NMC (6.15 vs 1.76, respectively). Also, TMC had less than a half sulfated polysaccharides compared to NMC. We conclude that subcutaneous tumor grafts mediate degranulation of MC from peritoneal cavity with the consequent release of MC mediators such as heparin. This may be one of the factors for the absence of antitumoral effect of TMC.
Registro:
| Documento: | Artículo |
| Título: | Microfluorometry and image analysis of peritoneal mast cells reveal differences in the morphology and content of sulfated polyanions between normal and tumor-bearing mice |
| Autor: | Bertolesi, G.E.; Stockert, J.C.; De Cidre, L.L. |
| Filiación: | Department of Biology, Fac. of Exact and Natural Sciences, University of Buenos Aires, Argentina Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Spain Cátedra de Histol. Animal, Departamento de Biología, Ciudad Universitataria, Buenos Aires 1428, Argentina |
| Palabras clave: | Adenocarcinoma; Heparin; Image analysis; Mast cell; Tris (2,2'-bipyridine) ruthenium (II); adenocarcinoma; animal cell; article; breast carcinoma; image analysis; mast cell degranulation; microfluorometry; mouse; nonhuman; peritoneum mast cell; priority journal; tumor growth |
| Año: | 1997 |
| Volumen: | 11 |
| Número: | 6 |
| Página de inicio: | 1221 |
| Página de fin: | 1225 |
| Título revista: | International Journal of Oncology |
| Título revista abreviado: | INT. J. ONCOL. |
| ISSN: | 10196439 |
| CODEN: | IJONE |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10196439_v11_n6_p1221_Bertolesi |
Referencias:
- Metcalfe, D.D., Mast cell mediators with emphasis on intestinal mast cells (1984) Allergy, 53, pp. 563-575
- Schwartz, L.B., Austen, K.F., Structure and function of the chemical mediators of mast cells (1984) Prog Allergy, 34, pp. 271-321
- Langer, J., Liebman, S., Monk, P.K., Pelletir, G.S., Mast cell mediators and peritoneal adhesion formation in the rats (1995) J Surg Res, 59, pp. 244-348
- Dabbous, M., Walker, R., Haney, L., Carter, L.M., Nicolson, G.L., Woolley, D.E., Mast cells and matrix degradation at sites of tumor invasion on rat mammary adenocarcinoma (1986) Br J Cancer, 54, pp. 459-464
- Roche, W., The nature and significance of tumor associated mast cells (1986) J Pathol, 148, pp. 175-182
- Rosengard, B.R., Mahalike, Cochrane, D., Mast cell secretion: Differences between immunologic and non immunologic stimulation (1986) Agents Actions, 19, pp. 3-4
Citas:
---------- APA ----------
Bertolesi, G.E., Stockert, J.C. & De Cidre, L.L. (1997) . Microfluorometry and image analysis of peritoneal mast cells reveal differences in the morphology and content of sulfated polyanions between normal and tumor-bearing mice. International Journal of Oncology, 11(6), 1221-1225.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10196439_v11_n6_p1221_Bertolesi [ ]
---------- CHICAGO ----------
Bertolesi, G.E., Stockert, J.C., De Cidre, L.L. "Microfluorometry and image analysis of peritoneal mast cells reveal differences in the morphology and content of sulfated polyanions between normal and tumor-bearing mice" . International Journal of Oncology 11, no. 6 (1997) : 1221-1225.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10196439_v11_n6_p1221_Bertolesi [ ]
---------- MLA ----------
Bertolesi, G.E., Stockert, J.C., De Cidre, L.L. "Microfluorometry and image analysis of peritoneal mast cells reveal differences in the morphology and content of sulfated polyanions between normal and tumor-bearing mice" . International Journal of Oncology, vol. 11, no. 6, 1997, pp. 1221-1225.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10196439_v11_n6_p1221_Bertolesi [ ]
---------- VANCOUVER ----------
Bertolesi, G.E., Stockert, J.C., De Cidre, L.L. Microfluorometry and image analysis of peritoneal mast cells reveal differences in the morphology and content of sulfated polyanions between normal and tumor-bearing mice. INT. J. ONCOL. 1997;11(6):1221-1225.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10196439_v11_n6_p1221_Bertolesi [ ]
