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Abstract:

Background/Aims: Reduction in renal mass by uninephrectomy induces a functional compensation in the remnant kidney. The activity of the angiotensin-converting enzyme (ACE) as well as renin mRNA in the proximal convoluted tubule (PCT) of uninephrectomized (UNx) rats increases. The aim of this work was to determine whether the increased activity of the local renin-angiotensin system (RAS) participates in the adaptation of renal function after uninephrectomy. Method: We utilized normal two-kidney (2K) and 3-week UNx rats to study the activity of the ACE in vesicles obtained from luminal membranes of proximal tubular cells and the acidification kinectics in PCTs using micropuncture techniques. Results: The converting enzyme activity was significantly larger in UNx (5.87 ± 0.69 nmol · min1 · mg protein-1) than in 2K rats (2.43 ± 0.13 nmol · min-1 · mg protein-1); p < 0.05). The acidification rate constant (κ) in PCT of 2K rats was 0.18 ± 0.02 s-1 and in UNx rats 0.30 ± 0.04 s-1 (p < 0.001). In UNx rats, microperfusion with 10-5 M ramipril or 10-5 M losartan decreased κ to 0.19 ± 0.02 and 0.18 ± 0.02 s-1, respectively, but had no effect on 2K rats. Luminal steady-state pH (pH(∞)) was the same in 2K and UNx rats, and was not modified by addition of 10-5 M ramipril or 10-5 M losartan in both groups. The proximal H+ flux (J(H+)), calculated from pH(∞) and κ, was 1.12 nmol · cm-2 · s-1 in 2K rats and, 1.77 nmol · cm-2 · s-1 in UNx rats (p < 0.001). In 2K rats, this value was not changed by 10-5 M ramipril or 10-5 M losartan, but in UNx rats J(H+) decreased 25 and 30% with ramipril or losartan, respectively (p < 0.001). Conclusions: These data suggest that the increase in the local RAS activity could be an adaptive change that contributes to maintain the homeostasis of body fluids after uninephrectomy. Copyright (C) 2000 S. Karger AG, Basel.

Registro:

Documento: Artículo
Título:Intrarenal renin-angiotensin system contributes to tubular acidification adaptation following uninephrectomy
Autor:Amorena, C.; Damasco, C.; Igarreta, P.; Maclaughlin, M.
Filiación:Instituto de Investigaciones Cardiológicas, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
CONICET, Departamento de Quimica Biológica, Universidad de Buenos Aires, Buenos Aires, Argentina
ECyT, Universidad Nacional de Gral San Martin, Argentina
Instituto de Investigaciones Cardiológicas, Marcelo T de Alvear 2270, 1122 Buenos Aires, Argentina
Palabras clave:Angiotensin; Converting enzyme; Micropuncture; Na+-H+; Proximal tubular acidification; Renin-angiotensin system; Uninephrectomy; dipeptidyl carboxypeptidase; losartan; messenger RNA; ramipril; acidification; adaptation; animal experiment; animal model; animal tissue; article; body fluid; brush border vesicle; controlled study; enzyme activity; homeostasis; kidney proximal tubule; male; nonhuman; pH; priority journal; punch biopsy; rat; renin angiotensin aldosterone system; uninephrectomy; Acids; Adaptation, Physiological; Angiotensin-Converting Enzyme Inhibitors; Animals; Kidney; Kidney Tubules, Proximal; Losartan; Male; Nephrectomy; Postoperative Period; Ramipril; Rats; Rats, Wistar; Receptor, Angiotensin, Type 1; Receptor, Angiotensin, Type 2; Receptors, Angiotensin; Renin-Angiotensin System
Año:2001
Volumen:9
Número:1
Página de inicio:60
Página de fin:64
Título revista:Experimental Nephrology
Título revista abreviado:Exp. Nephrol.
ISSN:10187782
CODEN:EXNEE
CAS:Acids; Angiotensin-Converting Enzyme Inhibitors; Losartan, 114798-26-4; Ramipril, 87333-19-5; Receptor, Angiotensin, Type 1; Receptor, Angiotensin, Type 2; Receptors, Angiotensin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10187782_v9_n1_p60_Amorena

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Citas:

---------- APA ----------
Amorena, C., Damasco, C., Igarreta, P. & Maclaughlin, M. (2001) . Intrarenal renin-angiotensin system contributes to tubular acidification adaptation following uninephrectomy. Experimental Nephrology, 9(1), 60-64.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10187782_v9_n1_p60_Amorena [ ]
---------- CHICAGO ----------
Amorena, C., Damasco, C., Igarreta, P., Maclaughlin, M. "Intrarenal renin-angiotensin system contributes to tubular acidification adaptation following uninephrectomy" . Experimental Nephrology 9, no. 1 (2001) : 60-64.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10187782_v9_n1_p60_Amorena [ ]
---------- MLA ----------
Amorena, C., Damasco, C., Igarreta, P., Maclaughlin, M. "Intrarenal renin-angiotensin system contributes to tubular acidification adaptation following uninephrectomy" . Experimental Nephrology, vol. 9, no. 1, 2001, pp. 60-64.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10187782_v9_n1_p60_Amorena [ ]
---------- VANCOUVER ----------
Amorena, C., Damasco, C., Igarreta, P., Maclaughlin, M. Intrarenal renin-angiotensin system contributes to tubular acidification adaptation following uninephrectomy. Exp. Nephrol. 2001;9(1):60-64.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10187782_v9_n1_p60_Amorena [ ]