Abstract:
We previously observed that Bacillus subtilis spores from sspE mutants presented a lower germination capacity in media containing high salt concentrations (0. 9 M NaCl). This deficiency was attributed to the absence of SASP-E (gamma-type small-acid-soluble protein), rich in osmocompatible amino acids released by degradation. Herein we observed that, in addition, this mutant spore presented a reduced capacity to use L-alanine as germinant (L-ala pathway), required longer times to germinate in calcium dipicolinate (Ca2+-DPA), but germinated well in asparagine, glucose, fructose, and potassium chloride (AGFK pathway). Moreover, mild sonic treatment of mutant spores partially recovered their germination capacity in L-ala. Spore qualities were also altered, since sporulating colonies from the sspE mutant showed a pale brownish color, a higher adherence to agar plates, and lower auto-fluorescence, properties related to their spore coat content. Furthermore, biochemical analysis showed a reduced partition in hexadecane and a higher content of Ca2+-DPA when compared with its isogenic wild-type control. Coat protein preparations showed a different electrophoretic pattern, in particular when detected with antibodies against CotG and CotE. The complementation with a wild-type sspE gene in a plasmid allowed for recovering the wild-type coat phenotype. This is the first report of a direct involvement of SASP-E in the spore coat assembly during the differentiation program of sporulation. © the Korean Society for Microbiology and Biotechnology.
Registro:
Documento: |
Artículo
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Título: | "A La Recherche" of functions for the spore protein SASP-E from Bacillus subtilis |
Autor: | Ruzal, S.M.; Bustos, P.L.; Sánchez-Rivas, C. |
Filiación: | Universidad de Buenos Aires, Facultad Ciencias Exactas y Naturales, Departamento de Química Biológica, Ciudad Universitaria Pabellón II, 4 piso, C1428EGA Buenos Aires, Argentina
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Palabras clave: | Bacillus subtilis; Coat; Germination; SASP-E; Spores; asparagine; bacterial protein; calcium; dipicolinic acid; fructose; gamma type small acid soluble protein; glucose; hexadecane; potassium chloride; unclassified drug; article; autofluorescence; Bacillus subtilis; bacterial gene; bacterium colony; controlled study; genetic complementation; nonhuman; phenotype; plasmid; protein determination; protein function; spore germination; sspE gene; wild type; Bacillus subtilis; Bacterial Proteins; Gene Deletion; Genetic Complementation Test; Protein Multimerization; Spores, Bacterial |
Año: | 2013
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Volumen: | 23
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Número: | 1
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Página de inicio: | 15
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Página de fin: | 21
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DOI: |
http://dx.doi.org/10.4014/jmb.1206.06028 |
Título revista: | Journal of Microbiology and Biotechnology
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Título revista abreviado: | J. Microbiol. Biotechnol.
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ISSN: | 10177825
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CODEN: | JOMBE
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CAS: | asparagine, 70-47-3, 7006-34-0; calcium, 14092-94-5, 7440-70-2; dipicolinic acid, 17606-33-6, 499-83-2; fructose, 30237-26-4, 57-48-7, 7660-25-5, 77907-44-9; glucose, 50-99-7, 84778-64-3; hexadecane, 544-76-3; potassium chloride, 7447-40-7; Bacterial Proteins; spore-specific proteins, Bacillus
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10177825_v23_n1_p15_Ruzal |
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Citas:
---------- APA ----------
Ruzal, S.M., Bustos, P.L. & Sánchez-Rivas, C.
(2013)
. "A La Recherche" of functions for the spore protein SASP-E from Bacillus subtilis. Journal of Microbiology and Biotechnology, 23(1), 15-21.
http://dx.doi.org/10.4014/jmb.1206.06028---------- CHICAGO ----------
Ruzal, S.M., Bustos, P.L., Sánchez-Rivas, C.
""A La Recherche" of functions for the spore protein SASP-E from Bacillus subtilis"
. Journal of Microbiology and Biotechnology 23, no. 1
(2013) : 15-21.
http://dx.doi.org/10.4014/jmb.1206.06028---------- MLA ----------
Ruzal, S.M., Bustos, P.L., Sánchez-Rivas, C.
""A La Recherche" of functions for the spore protein SASP-E from Bacillus subtilis"
. Journal of Microbiology and Biotechnology, vol. 23, no. 1, 2013, pp. 15-21.
http://dx.doi.org/10.4014/jmb.1206.06028---------- VANCOUVER ----------
Ruzal, S.M., Bustos, P.L., Sánchez-Rivas, C. "A La Recherche" of functions for the spore protein SASP-E from Bacillus subtilis. J. Microbiol. Biotechnol. 2013;23(1):15-21.
http://dx.doi.org/10.4014/jmb.1206.06028