Artículo

Allievi; Claudia, M.; Florencia, S.; Mariano, P.-A.; Mercedes, P.M.; Ruzal, S.M.; Carmen, S.-R. "Metal biosorption by surface-layer proteins from Bacillus species" (2011) Journal of Microbiology and Biotechnology. 21(2):147-153
La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Bacillus species have been involved in metal association as biosorbents, but there is not a clear understanding of this chelating property. In order to evaluate this metal chelating capacity, cultures and spores from Grampositive bacteria of species either able or unable to produce surface layer proteins (S-layers) were analyzed for their capacity of copper biosorption. Only those endowed of S-layers, like Bacillus sphaericus and B. thuringiensis, showed a significant biosorption capacity. This capacity (nearly 50%) was retained after heating of cultures, thus supporting that structural elements of the envelopes are responsible for such activity. Purified Slayers from two Bacillus sphaericus strains had the ability to biosorb copper. Copper biosorption parameters were determined for strain B. sphaericus 2362, and after analyses by means of the Langmuir model, the affinity and capacity were shown to be comparable to other bacterial biosorbents. A competitive effect of Ca2+ and Zn2+, but not of Cd2+, was also observed, thus indicating that other cations may be biosorbed by this protein. Spores that have been shown to be proficient for copper biosorption were further analyzed for the presence of Slayer content. The retention of S-layers by these spores was clearly observed, and after extensive treatment to eliminate the S-layers, the biosorption capacity of these spores was significantly reduced. For the first time, a direct correlation between S-layer protein content and metal biosorption capacity is shown. This capacity is linked to the retention of S-layer proteins attached to Bacillus spores and cells. © 2009 by the Korean Society for Microbiology and Biotechnology.

Registro:

Documento: Artículo
Título:Metal biosorption by surface-layer proteins from Bacillus species
Autor:Allievi; Claudia, M.; Florencia, S.; Mariano, P.-A.; Mercedes, P.M.; Ruzal, S.M.; Carmen, S.-R.
Filiación:Universidad de Buenos Aires, Facultad Ciencias Exactas y Naturales, Departamento de Química Biológica, Cdad, Universitaria Pabellón II, 4 piso, C1428EGA, Buenos Aires, Argentina
Palabras clave:Bacillus; Metal biosorption; S-layer; Spores; bacterial protein; cadmium; calcium ion; copper; membrane protein; zinc ion; article; Bacillus sphaericus; Bacillus thuringiensis; bacterial membrane; bacterial metabolism; bacterial spore; bacterial strain; bacterium culture; biosorption; chelation; controlled study; heating; metal binding; nonhuman; protein content; protein localization; whole cell; Bacillus; Cations, Divalent; Chelating Agents; Membrane Glycoproteins; Metals; Protein Binding; Spores, Bacterial; Bacillus (bacterium); Bacillus sphaericus; Bacillus thuringiensis; Bacteria (microorganisms)
Año:2011
Volumen:21
Número:2
Página de inicio:147
Página de fin:153
DOI: http://dx.doi.org/10.4014/jmb.1009.09046
Título revista:Journal of Microbiology and Biotechnology
Título revista abreviado:J. Microbiol. Biotechnol.
ISSN:10177825
CODEN:JOMBE
CAS:cadmium, 22537-48-0, 7440-43-9; calcium ion, 14127-61-8; copper, 15158-11-9, 7440-50-8; zinc ion, 23713-49-7; Cations, Divalent; Chelating Agents; Membrane Glycoproteins; Metals; S-layer proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10177825_v21_n2_p147_Allievi

Referencias:

  • Andreazza, R., Pieniz, S., Wolf, L., Lee, M.K., Camargo, F.A., Okeke, B.C., Characterization of copper bioreduction and biosorption by a highly copper resistant bacterium isolated from copper-contaminated vineyard soil (2010) Sci. Total Environ, 408, pp. 1501-1507
  • Brenner, A.J., Harris, E.D., A quantitative test for copper using bicinchoninic acid (1995) Anal. Biochem, 226, pp. 80-84. , Published erratum appears in Anal. Biochem
  • Chung, L., Rajan, K.S., Merdinger, E., Grecz, N., Coordinative binding of divalent cations with ligands related to bacterial spores (1971) Equilibrium Studies. Biophys. J, 11, pp. 469-482
  • Cucchi, A., Sanchez Rivas, C., Sensitivity of growing cells and spores of Bacillus thuringiensis var. israelensis and Bacillus sphaericus to osmotic variations (1994) Curr. Microbiol, 28, pp. 123-127
  • Dick, G.J., Torpey, J.W., Beveridge, T.J., Tebo, B.M., Direct identification of a bacterial manganese(II) oxidase, the multicopper oxidase MnxG, from spores of several different marine Bacillus species (2008) Appl. Environ. Microbiol, 74, pp. 1527-1534
  • Driks, A., Bacillus subtilis spore coat (1999) Microbiol. Mol. Biol. Rev, 63, pp. 1-20
  • Engelhardt, H., Are S-layers exoskeletons? The basic function of protein surface layers revisited (2007) J. Struct. Biol, 160, pp. 115-124
  • Francis, C.A., Casciotti, K.L., Tebo, B.M., Localization of Mn(II)-oxidizing activity and the putative multicopper oxidase, MnxG, to the exosporium of the marine Bacillus sp. strain SG-1 (2002) Arch. Microbiol, 178, pp. 450-456
  • Garavaglia, L., Cerdeira, S.B., Vullo, D.L., Chromium (VI) biotransformation by beta- and gamma-Proteobacteria from natural polluted environments: A combined biological and chemical treatment for industrial wastes (2010) J. Hazard Mater, 175, pp. 104-110
  • Guo, G., Zhang, L., Zhou, Z., Ma, Q., Liu, J., Zhu, C., Zhu, L., Sun, M., A new group of parasporal inclusions encoded by the S-layer gene of Bacillus thuringiensis (2008) FEMS Microbiol. Lett, 282, pp. 1-7
  • He, L.M., Tebo, B.M., Surface charge properties of and Cu(II) adsorption by spores of the marine Bacillus sp. strain SG-1 (1998) Appl. Environ. Microbiol, 64, pp. 1123-1129
  • Hullo, M.F., Moszer, I., Danchin, A., Martin-Verstraete, I., CotA of Bacillus subtilis is a copper-dependent laccase (2001) J. Bacteriol, 183, pp. 5426-5430
  • Jakava-Viljanen, M., Avall-Jaaskelainen, S., Messner, P., Sleytr, U.B., Palva, A., Isolation of three new surface layer protein genes (slp) from Lactobacillus brevis ATCC 14869 and characterization of the change in their expression under aerated and anaerobic conditions (2002) J. Bacteriol, 184, pp. 6786-6795
  • Kern, J.W., Schneewind, O., BslA, a pXO1-encoded adhesin of Bacillus anthracis (2008) Mol. Microbiol, 68, pp. 504-515
  • Lewis, L.O., Yousten, A.A., Murray, R.G., Characterization of the surface protein layers of the mosquitopathogenic strains of Bacillus sphaericus (1987) J. Bacteriol, 169, pp. 72-79
  • Liu, M., Li, S., Hu, S., Zhao, C., Bi, D., Sun, M., Display of avian influenza virus nucleoprotein on Bacillus thuringiensis cell surface using CTC as a fusion partner (2008) Appl. Microbiol. Biotechnol, 78, pp. 669-676
  • Lo, W., Ng, L.M., Chua, H., Yu, P.H., Sin, S.N., Wong, P.K., Biosorption and desorption of copper(II) ions by Bacillus sp (2003) Appl. Biochem. Biotechnol, pp. 105-108+581-591
  • Merroun, M.L., Raff, J., Rossberg, A., Hennig, C., Reich, T., Selenska-Pobell, S., Complexation of uranium by cells and S-layer sheets of Bacillus sphaericus JG-A12 (2005) Appl. Environ. Microbiol, 71, pp. 5532-5543
  • Mignot, T., Mesnage, S., Couture-Tosi, E., Mock, M., Fouet, A., Developmental switch of S-layer protein synthesis in Bacillus anthracis (2002) Mol. Microbiol, 43, pp. 1615-1627
  • Pollmann, K., Matys, S., Construction of an S-layer protein exhibiting modified self-assembling properties and enhanced metal binding capacities (2007) Appl. Microbiol. Biotechnol, 75, pp. 1079-1085
  • Pollmann, K., Raff, J., Merroun, M., Fahmy, K., Selenska- Pobell, S., Metal binding by bacteria from uranium mining waste piles and its technological applications (2006) Biotechnol. Adv, 24, pp. 58-68
  • Prado Acosta, M., Valdman, E., Battaglini, F., Leite, S., Ruzal, S.M., Biosorption of copper by Paenibacillus polymyxa cells and their exopolysaccharide (2005) World J. Microbiol. Biotechnol, 21, pp. 1157-1163
  • Prado Acosta, M., Ruzal, S.M., Allievi, M.C., Palomino, M.M., Sanchez Rivas, C., Synergistic effects of the Lactobacillus acidophilus surface layer and nisin on bacterial growth (2010) Appl. Environ. Microbiol, 76, pp. 974-977
  • Sandman, K., Losick, R., Youngman, P., Genetic analysis of Bacillus subtilis spo mutations generated by Tn917- mediated insertional mutagenesis (1987) Genetics, 117, pp. 603-617
  • Sára, M., Sleytr, U.B., S-Layer proteins (2000) J. Bacteriol, 182, pp. 859-868
  • Selenska-Pobell, S., Panak, P., Miteva, V., Boudakov, I., Bernhard, G., Nitsche, H., Selective accumulation of heavy metals by three indigenous Bacillus strains. B cereus, B megaterium from drain waters of a uranium waste pile (1999) FEMS Microbiol. Ecol, 29, pp. 59-67
  • Smit, E., Oling, F., Demel, R., Martinez, B., Pouwels, P.H., The S-layer protein of Lactobacillus acidophilus ATCC 4356: Identification and characterisation of domains responsible for S-protein assembly and cell wall binding (2001) J. Mol. Biol, 305, pp. 245-257
  • Tsekova, K., Todorova, D., Dencheva, V., Ganeva, S., Biosorption of copper(II) and cadmium(II) from aqueous solutions by free and immobilized biomass of Aspergillus niger (2010) Bioresour. Technol, 101, pp. 1727-1731
  • Velasquez, L., Dussan, J., Biosorption and bioaccumulation of heavy metals on dead and living biomass of Bacillus sphaericus (2009) J. Hazard. Mater, 167, pp. 713-716
  • Williams, D.D., Turnbough, C.L., Surface layer protein EA1 is not a component of Bacillus anthracis spores but is a persistent contaminant in spore preparations (2004) J. Bacteriol, 186, pp. 566-569

Citas:

---------- APA ----------
Allievi, Claudia, M., Florencia, S., Mariano, P.-A., Mercedes, P.M., Ruzal, S.M. & Carmen, S.-R. (2011) . Metal biosorption by surface-layer proteins from Bacillus species. Journal of Microbiology and Biotechnology, 21(2), 147-153.
http://dx.doi.org/10.4014/jmb.1009.09046
---------- CHICAGO ----------
Allievi, Claudia, M., Florencia, S., Mariano, P.-A., Mercedes, P.M., Ruzal, S.M., et al.. "Metal biosorption by surface-layer proteins from Bacillus species" . Journal of Microbiology and Biotechnology 21, no. 2 (2011) : 147-153.
http://dx.doi.org/10.4014/jmb.1009.09046
---------- MLA ----------
Allievi, Claudia, M., Florencia, S., Mariano, P.-A., Mercedes, P.M., Ruzal, S.M., et al.. "Metal biosorption by surface-layer proteins from Bacillus species" . Journal of Microbiology and Biotechnology, vol. 21, no. 2, 2011, pp. 147-153.
http://dx.doi.org/10.4014/jmb.1009.09046
---------- VANCOUVER ----------
Allievi, Claudia, M., Florencia, S., Mariano, P.-A., Mercedes, P.M., Ruzal, S.M., et al.. Metal biosorption by surface-layer proteins from Bacillus species. J. Microbiol. Biotechnol. 2011;21(2):147-153.
http://dx.doi.org/10.4014/jmb.1009.09046