Artículo

Cacace, V.I.; Finkelsteyn, A.G.; Tasso, L.M.; Kusnier, C.F.; Gomez, K.A.; Fischbarg, J. "Regulatory volume increase and regulatory volume decrease responses in HL-1 atrial myocytes" (2014) Cellular Physiology and Biochemistry. 33(6):1745-1757
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Abstract:

Background/Aims: we have investigated whether cultured cardiomyocytes of the cell line HL-1 have the ability to perform regulatory volume responses both in hypotonic and hypertonic conditions. Furthermore, we characterized those regulatory responses and studied the effects of bumetanide and DIDS in volume regulation of HL-1 cells. Methods: we used a light scattering system to measure the transient volume changes of HL-1 cells when subjected to osmotic challenge. Results: We found that HL-1 cells correct for their volume excess by undergoing regulatory volume decrease (RVD), and also respond to hypertonic stress with a regulatory volume increase (RVI). Rate of RVD was 0.08 ± 0.04 intensity/min, and rate of RVI was 0.09 ± 0.01 intensity/min. Volume recovery was 83.68 ± 5.73 % for RVD and 92.3 ± 2.3 % for RVI. Bumetanide 50 μM inhibited volume recovery, from 92.3 ± 2.3 % (control) to 24.6 ± 8.8 % and reduced the rate of RVI from 0.070 ± 0.020 intensity/min (control) to 0.010 ± 0.005 intensity/min. 50 μM DIDS reduced volume recovery to 42.93 ± 7.7 % and rate of RVI, to 0.03 ± 0.01 intensity/min. Conclusions: these results suggest that bumetanide- and DIDS-sensitive mechanisms are involved in the RVI of HL-1 cells, which points to the involvement of the Na+/K+/2Cl- cotransporter and Cl-/bicarbonate exchanger in RVI, respectively. © 2014 S. Karger AG, Basel.

Registro:

Documento: Artículo
Título:Regulatory volume increase and regulatory volume decrease responses in HL-1 atrial myocytes
Autor:Cacace, V.I.; Finkelsteyn, A.G.; Tasso, L.M.; Kusnier, C.F.; Gomez, K.A.; Fischbarg, J.
Filiación:Instituto de Investigaciones Cardiológicas 'Profesor Alberto Taquini' (ININCA), Facultad de Medicina, Universidad de Buenos Aires, Marcelo T. de Alvear 2270, Buenos Aires 1122, Argentina
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina
Instituto Del Cálculo, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Bumetanide; Cell volume regulation; DIDS; HL-1 atrial myocytes; RVD; RVI; 4,4' diisothiocyanatostilbene 2,2' disulfonic acid; bumetanide; hypertonic solution; hypotonic solution; sodium potassium chloride cotransporter; bicarbonate chloride antiporter; isotonic solution; Ringer solution; sodium potassium chloride cotransporter; 4,4' diisothiocyanatostilbene 2,2' disulfonic acid; bumetanide; diuretic agent; hypertonic solution; hypotonic solution; animal cell; animal experiment; article; biological activity; cell function; cell membrane transport; cell swelling; cell volume; cellular parameters; concentration response; enzyme inhibition; heart muscle cell; hyperosmotic stress; hypoosmotic stress; light intensity; light scattering; nonhuman; osmosis; osmotic stress; priority journal; regulatory mechanism; regulatory volume decrease; regulatory volume increase; Article; cell membrane; controlled study; illumination; mouse; water flow; animal; cardiac muscle cell; cell line; cell size; cytology; drug effects; time factor; 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; Animals; Bumetanide; Cell Line; Cell Size; Diuretics; Hypertonic Solutions; Hypotonic Solutions; Mice; Myocytes, Cardiac; Osmosis; Time Factors
Año:2014
Volumen:33
Número:6
Página de inicio:1745
Página de fin:1757
DOI: http://dx.doi.org/10.1159/000362955
Título revista:Cellular Physiology and Biochemistry
Título revista abreviado:Cell. Physiol. Biochem.
ISSN:10158987
CODEN:CEPBE
CAS:4,4' diisothiocyanatostilbene 2,2' disulfonic acid, 53005-05-3; bumetanide, 28395-03-1; Ringer solution, 8026-10-6; 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; Bumetanide; Diuretics; Hypertonic Solutions; Hypotonic Solutions
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10158987_v33_n6_p1745_Cacace

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Citas:

---------- APA ----------
Cacace, V.I., Finkelsteyn, A.G., Tasso, L.M., Kusnier, C.F., Gomez, K.A. & Fischbarg, J. (2014) . Regulatory volume increase and regulatory volume decrease responses in HL-1 atrial myocytes. Cellular Physiology and Biochemistry, 33(6), 1745-1757.
http://dx.doi.org/10.1159/000362955
---------- CHICAGO ----------
Cacace, V.I., Finkelsteyn, A.G., Tasso, L.M., Kusnier, C.F., Gomez, K.A., Fischbarg, J. "Regulatory volume increase and regulatory volume decrease responses in HL-1 atrial myocytes" . Cellular Physiology and Biochemistry 33, no. 6 (2014) : 1745-1757.
http://dx.doi.org/10.1159/000362955
---------- MLA ----------
Cacace, V.I., Finkelsteyn, A.G., Tasso, L.M., Kusnier, C.F., Gomez, K.A., Fischbarg, J. "Regulatory volume increase and regulatory volume decrease responses in HL-1 atrial myocytes" . Cellular Physiology and Biochemistry, vol. 33, no. 6, 2014, pp. 1745-1757.
http://dx.doi.org/10.1159/000362955
---------- VANCOUVER ----------
Cacace, V.I., Finkelsteyn, A.G., Tasso, L.M., Kusnier, C.F., Gomez, K.A., Fischbarg, J. Regulatory volume increase and regulatory volume decrease responses in HL-1 atrial myocytes. Cell. Physiol. Biochem. 2014;33(6):1745-1757.
http://dx.doi.org/10.1159/000362955