Artículo

Moiola, C.; De Luca, P.; Cotignola, J.; Gardner, K.; Vazquez, E.; De Siervi, A. "Dynamic coregulatory complex containing BRCA1, E2F1 and CtIP controls ATM transcription" (2012) Cellular Physiology and Biochemistry. 30(3):596-608
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Abstract:

Chromosomal instability is a key feature in cancer progression. Recently we have reported that BRCA1 regulates the transcription of several genes in prostate cancer, including ATM (ataxia telangiectasia mutated). Although it is well accepted that ATM is a pivotal mediator in genotoxic stress, it is unknown whether ATM transcription is regulated during the molecular response to DNA damage. Here we investigate ATM transcription regulation in human prostate tumor PC3 cell line. We have found that doxorubicin and mitoxantrone repress ATM transcription in PC3 cells but etoposide and methotrexate do not affect ATM expression. We have demonstrated that BRCA1 binds to ATM promoter and after doxorubicin exposure, it is released. BRCA1 overexpression increases ATM transcription and this enhancement is abolished by BRCA1 depletion. Moreover, BRCA1-BRCT domain loss impairs the ability of BRCA1 to regulate ATM promoter activity, strongly suggesting that BRCT domain is essential for ATM regulation by BRCA1. BRCA1-overexpressing PC3 cells exposed to KU55933 ATM kinase inhibitor showed significant decreased ATM promoter activity compared to untreated cells, suggesting that ATM transcriptional regulation by BRCA1 is partially mediated by the ATM kinase activity. In addition, we have demonstrated E2F1 binding to ATM promoter before and after doxorubicin exposure. E2F1 overexpression diminishes ATM transcription after doxorubicin exposure which is impaired by E2F1 dominant negative mutants. Finally, the co-regulator of transcription CtIP increases ATM transcription. CtIP increases ATM transcription. Altogether, BRCA1/E2F1/CtIP binding to ATM promoter activates ATM transcription. Doxorubicin exposure releases BRCA1 and CtIP from ATM promoter still keeping E2F1 recruited and, in turn, represses ATM expression. Copyright © 2012 S. Karger AG, Basel.

Registro:

Documento: Artículo
Título:Dynamic coregulatory complex containing BRCA1, E2F1 and CtIP controls ATM transcription
Autor:Moiola, C.; De Luca, P.; Cotignola, J.; Gardner, K.; Vazquez, E.; De Siervi, A.
Filiación:Department of Biological Chemistry, School of Sciences (FCEN), University of Buenos Aires (UBA), Buenos Aires, Argentina
Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States
Intendente Guiraldes 2160 Pab II, 2do Piso, CM1, Departamento de Química Biológica, Universidad de Buenos Aires, Buenos Aires, C1428EGA, Argentina
Palabras clave:ATM; BRCA1; DNA damage; Prostate cancer; Transcription; 2 morpholino 6 (1 thianthrenyl) 4 pyranone; ATM protein; BRCA1 associated ring domain protein 1; C terminal interacting protein; doxorubicin; etoposide; histone H2AX; methotrexate; mitoxantrone; protein; transcription factor E2F1; unclassified drug; article; binding site; cancer cell culture; chromatin; controlled study; DNA damage; genetic transcription; human; human cell; male; positive feedback; priority journal; promoter region; prostate cancer; protein binding; protein expression; protein phosphorylation; protein secretion; transcription regulation; Antibiotics, Antineoplastic; BRCA1 Protein; Carrier Proteins; Cell Cycle Proteins; Cell Line, Tumor; DNA Damage; DNA Repair; DNA-Binding Proteins; Doxorubicin; E2F1 Transcription Factor; Humans; Morpholines; Nuclear Proteins; Promoter Regions, Genetic; Protein Binding; Protein Structure, Tertiary; Protein-Serine-Threonine Kinases; Pyrones; Transcription, Genetic; Tumor Suppressor Proteins; Ataxia telangiectasia
Año:2012
Volumen:30
Número:3
Página de inicio:596
Página de fin:608
DOI: http://dx.doi.org/10.1159/000341441
Título revista:Cellular Physiology and Biochemistry
Título revista abreviado:Cell. Physiol. Biochem.
ISSN:10158987
CODEN:CEPBE
CAS:2 morpholino 6 (1 thianthrenyl) 4 pyranone, 587871-26-9; doxorubicin, 23214-92-8, 25316-40-9; etoposide, 33419-42-0; methotrexate, 15475-56-6, 59-05-2, 7413-34-5; mitoxantrone, 65271-80-9, 70476-82-3; protein, 67254-75-5; 2-morpholin-4-yl-6-thianthren-1-yl-pyran-4-one; Antibiotics, Antineoplastic; BRCA1 Protein; Carrier Proteins; Cell Cycle Proteins; DNA-Binding Proteins; Doxorubicin, 23214-92-8; E2F1 Transcription Factor; Morpholines; Nuclear Proteins; Protein-Serine-Threonine Kinases, 2.7.11.1; Pyrones; RBBP8 protein, human; Tumor Suppressor Proteins; ataxia telangiectasia mutated protein, 2.7.11.1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10158987_v30_n3_p596_Moiola

Referencias:

  • Jemal, A., Bray, F., Center, M.M., Ferlay, J., Ward, E., Forman, D., Global cancer statistics (2011) CA Cancer J Clin, 61, p. 134
  • Schulz, W.A., Burchardt, M., Cronauer, M.V., Molecular biology of prostate cancer (2003) Mol Hum Reprod, 9, pp. 437-448
  • Bickers, B., Aukim-Hastie, C., New molecular biomarkers for the prognosis and management of prostate cancer - The post PSA era (2009) Anticancer Res, 29, pp. 3289-3298
  • Hanahan, D., Weinberg, R.A., Hallmarks of cancer: The next generation (2011) Cell, 144, pp. 646-674
  • Truman, J.P., Rotenberg, S.A., Kang, J.H., Lerman, G., Fuks, Z., Kolesnick, R., Marquez, V.E., Haimovitz-Friedman, A., PKCalpha activation downregulates ATM and radio-sensitizes androgen-sensitive human prostate cancer cells in vitro and in vivo (2009) Cancer Biol Ther, 8, pp. 54-63
  • Fan, R., Kumaravel, T.S., Jalali, F., Marrano, P., Squire, J.A., Bristow, R.G., Defective DNA strand break repair after DNA damage in prostate cancer cells: Implications for genetic instability and prostate cancer progression (2004) Cancer Res, 64, pp. 8526-8533
  • Navone, N.M., Troncoso, P., Pisters, L.L., Goodrow, T.L., Palmer, J.L., Nichols, W.W., Von Eschenbach, A.C., Conti, C.J., P53 protein accumulation and gene mutation in the progression of human prostate carcinoma (1993) J Natl Cancer Inst, 85, pp. 1657-1669
  • Barbazetto, I.A., Room, M., Yannuzzi, N.A., Barile, G.R., Merriam, J.E., Bardal, A.M., Freund, K.B., Allikmets, R., ATM gene variants in patients with idiopathic perifoveal telangiectasia (2008) Invest Ophthalmol Vis Sci, 49, pp. 3806-3811
  • Taylor, A.M., Metcalfe, J.A., Thick, J., Mak, Y.F., Leukemia and lymphoma in ataxia telangiectasia (1996) Blood, 87, pp. 423-438
  • Swift, M., Reitnauer, P.J., Morrell, D., Chase, C.L., Breast and other cancers in families with ataxia-telangiectasia (1987) N Engl J Med, 316, pp. 1289-1294
  • Berkovich, E., Ginsberg, D., ATM is a target for positive regulation by E2F-1 (2003) Oncogene, 22, pp. 161-167
  • Keating, K.E., Gueven, N., Watters, D., Rodemann, H.P., Lavin, M.F., Transcriptional downregulation of ATM by EGF is defective in ataxia-telangiectasia cells expressing mutant protein (2001) Oncogene, 20, pp. 4281-4290
  • Deng, C.X., BRCA1: Cell cycle checkpoint, genetic instability, DNA damage response and cancer evolution (2006) Nucleic Acids Res, 34, pp. 1416-1426
  • Rosen, E.M., Fan, S., Ma, Y., BRCA1 regulation of transcription (2006) Cancer Lett, 236, pp. 175-185
  • Mullan, P.B., Quinn, J.E., Harkin, D.P., The role of BRCA1 in transcriptional regulation and cell cycle control (2006) Oncogene, 25, pp. 5854-5863
  • Di, L.J., Fernandez, A.G., De Siervi, A., Longo, D.L., Gardner, K., Transcriptional regulation of BRCA1 expression by a metabolic switch (2010) Nat Struct Mol Biol, 17, pp. 1406-1413
  • De Siervi, A., De Luca, P., Byun, J.S., Di, L.J., Fufa, T., Haggerty, C.M., Vazquez, E., Gardner, K., Transcriptional autoregulation by BRCA1 (2010) Cancer Res, 70, pp. 532-542
  • Yun, M.H., Hiom, K., Understanding the functions of BRCA1 in the DNA-damage response (2009) Biochem Soc Trans, 37, pp. 597-604
  • De Luca, P., Vazquez, E.S., Moiola, C.P., Zalazar, F., Cotignola, J., Gueron, G., Gardner, K., De Siervi, A., BRCA1 loss induces GADD153-mediated doxorubicin resistance in prostate cancer (2011) Mol Cancer Res, 9, pp. 1078-1090
  • Smith, J.L., Freebern, W.J., Collins, I., De Siervi, A., Montano, I., Haggerty, C.M., McNutt, M.C., Gardner, K., Kinetic profiles of p300 occupancy in vivo predict common features of promoter structure and coactivator recruitment (2004) Proc Natl Acad Sci USA, 101, pp. 11554-11559
  • De Siervi, A., De Luca, P., Moiola, C., Gueron, G., Tongbai, R., Chandramouli, G.V., Haggerty, C., Gardner, K., Identification of new Rel/NFkB regulatory networks by focused genome location analysis (2009) Cell Cycle, 8, pp. 2093-2100
  • Gatei, M., Scott, S.P., Filippovitch, I., Soronika, N., Lavin, M.F., Weber, B., Khanna, K.K., Role for ATM in DNA damage-induced phosphorylation of BRCA1 (2000) Cancer Res, 60, pp. 3299-3304
  • Hickson, I., Zhao, Y., Richardson, C.J., Green, S.J., Martin, N.M., Orr, A.I., Reaper, P.M., Smith, G.C., Identification and characterization of a novel and specific inhibitor of the ataxia-telangiectasia mutated kinase ATM (2004) Cancer Res, 64, pp. 9152-9159
  • Liao, C.C., Tsai, C.Y., Chang, W.C., Lee, W.H., Wang, J.M., RB.E2F1 complex mediates DNA damage responses through transcriptional regulation of ZBRK1 (2010) J Biol Chem, 285, pp. 33134-33143
  • Yu, X., Wu, L.C., Bowcock, A.M., Aronheim, A., Baer, R., The C-terminal (BRCT) domains of BRCA1 interact in vivo with Ct IP, a protein implicated in the CtBP pathway of transcriptional repression (1998) J Biol Chem, 273, pp. 25388-25392
  • Zhou, B.B., Elledge, S.J., The DNA damage response: Putting checkpoints in perspective (2000) Nature, 408, pp. 433-439
  • Lieberman, H.B., DNA damage repair and response proteins as targets for cancer therapy (2008) Curr Med Chem, 15, pp. 360-367
  • Angèle, S., Falconer, A., Edwards, S.M., Dörk, T., Bremer, M., Moullan, N., Chapot, B., Hall, J., ATM polymorphisms as risk factors for prostate cancer development (2004) Br J Cancer, 91, pp. 783-787
  • Angele, S., Falconer, A., Foster, C.S., Taniere, P., Eeles, R.A., Hall, J., ATM protein overexpression in prostate tumors: Possible role in telomere maintenance (2004) Am J Clin Pathol, 121, pp. 231-236
  • Douglas, J.A., Levin, A.M., Zuhlke, K.A., Ray, A.M., Johnson, G.R., Lange, E.M., Wood, D.P., Cooney, K.A., Common variation in the BRCA1 gene and prostate cancer risk (2007) Cancer Epidemiol Biomarkers Prev, 16, pp. 1510-1516
  • Gallagher, D.J., Gaudet, M.M., Pal, P., Kirchhoff, T., Balistreri, L., Vora, K., Bhatia, J., Offit, K., Germline BRCA mutations denote a clinicopathologic subset of prostate cancer (2010) Clin Cancer Res, 16, pp. 2115-2121

Citas:

---------- APA ----------
Moiola, C., De Luca, P., Cotignola, J., Gardner, K., Vazquez, E. & De Siervi, A. (2012) . Dynamic coregulatory complex containing BRCA1, E2F1 and CtIP controls ATM transcription. Cellular Physiology and Biochemistry, 30(3), 596-608.
http://dx.doi.org/10.1159/000341441
---------- CHICAGO ----------
Moiola, C., De Luca, P., Cotignola, J., Gardner, K., Vazquez, E., De Siervi, A. "Dynamic coregulatory complex containing BRCA1, E2F1 and CtIP controls ATM transcription" . Cellular Physiology and Biochemistry 30, no. 3 (2012) : 596-608.
http://dx.doi.org/10.1159/000341441
---------- MLA ----------
Moiola, C., De Luca, P., Cotignola, J., Gardner, K., Vazquez, E., De Siervi, A. "Dynamic coregulatory complex containing BRCA1, E2F1 and CtIP controls ATM transcription" . Cellular Physiology and Biochemistry, vol. 30, no. 3, 2012, pp. 596-608.
http://dx.doi.org/10.1159/000341441
---------- VANCOUVER ----------
Moiola, C., De Luca, P., Cotignola, J., Gardner, K., Vazquez, E., De Siervi, A. Dynamic coregulatory complex containing BRCA1, E2F1 and CtIP controls ATM transcription. Cell. Physiol. Biochem. 2012;30(3):596-608.
http://dx.doi.org/10.1159/000341441