Artículo

La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

In this work, we studied the in vitro interactions between aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) and nitric oxide (NO), as well as the interactions between ALA, porphyrins and some NO donors and precursors. We employed three murine adenocarcinoma cell lines: LM2, which does not produce NO; LM3, which produces NO, and LM3-SNP, a variant of LM3 resistant to NO producing the same amount of NO as the parental. We did not find cross-resistance between NO-induced cytotoxicity and ALA-PDT. In spite of the lower porphyrin synthesis, LM2 cells show the highest sensitivity to ALA-PDT. However, we hypothesised that this is not related to the lack of endogenous NO production, because modulation of NO levels did not modify the response to PDT in any of the cell lines. Two unexpected results were found: the enhancement of NO production from the donor sodium nitroprusside (SNP) induced by ALA in both cells and medium, and the inhibition by ALA of NO production from arginine. We also found that SNP strongly protected the cells from ALA-PDT by impairing porphyrin biosynthesis as a consequence of an inhibition of the enzyme ALA dehydratase. We were not able to evaluate the action of NO derived from SNP because of the unexpected porphyrin impairment. On the other hand, impairment of NO from Arginine driven by ALA, although not modulating in vitro the ALA-PDT response, by increasing in vivo blood flow, may be contributing to the mechanism of tumour cures. © 2005 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity
Autor:Di Venosa, G.; Perotti, C.; Fukuda, H.; Batlle, A.; Casas, A.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias, FCEN, University of Buenos Aires and CONICET, Pabellón II, 2do Piso, 1428 Buenos Aires, Argentina
Viamonte 1881 10A, 1056 Buenos Aires, Argentina
Palabras clave:5-Aminolevulinic acid; ALA-PDT; Nitric oxide; Photodynamic therapy; aminolevulinic acid; arginine; hydrolyase; nitric oxide; nitric oxide donor; nitroprusside sodium; adenocarcinoma; animal cell; animal experiment; animal model; article; biosynthesis; blood flow; cancer therapy; carcinoma cell; cell protection; controlled study; cross resistance; cytotoxicity; drug mechanism; drug response; enzyme inhibition; hypothesis; in vitro study; in vivo study; molecular interaction; mouse; nonhuman; photodynamic therapy; priority journal; sensitivity analysis; Adenocarcinoma; Aminolevulinic Acid; Animals; Cell Line; Cell Line, Tumor; Cell Survival; Female; L Cells (Cell Line); Mammary Neoplasms, Animal; Mice; Mice, Inbred BALB C; Nitric Oxide; Nitric Oxide Donors; Photochemotherapy; Porphyrins; Murinae
Año:2005
Volumen:80
Número:3
Página de inicio:195
Página de fin:202
DOI: http://dx.doi.org/10.1016/j.jphotobiol.2005.05.001
Título revista:Journal of Photochemistry and Photobiology B: Biology
Título revista abreviado:J. Photochem. Photobiol. B Biol.
ISSN:10111344
CODEN:JPPBE
CAS:aminolevulinic acid, 106-60-5; arginine, 1119-34-2, 15595-35-4, 7004-12-8, 74-79-3; hydrolyase, 9044-86-4; nitric oxide, 10102-43-9; nitroprusside sodium, 14402-89-2, 15078-28-1; Aminolevulinic Acid, 106-60-5; Nitric Oxide Donors; Nitric Oxide, 10102-43-9; Porphyrins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10111344_v80_n3_p195_DiVenosa

Referencias:

  • Dougherty, T., Photodynamic therapy (PDT) of malignant tumors (1984) Crit. Rev. Oncol. Hematol., 2, pp. 83-116
  • Kennedy, J., Pottier, R., Pross, G., Photodynamic therapy with endogenous protoporphyrin IX: Basic principles and present clinical experience (1990) J. Photochem. Photobiol. B, 6, pp. 143-148
  • Fukuda, H., Casas, A., Chueke, F., Paredes, S., Batlle, A., Photodynamic action of endogenously synthesized porphyrins from aminolevulinic acid, using a new model for assaying the effectiveness of tumoral cell killing (1993) Int. J. Biochem., 25, pp. 1395-1398
  • Moncada, S., Palmer, R., Higgs, E., Nitric oxide: Physiology, phatology and pharmacology (1991) Pharmacol. Rev., 43, pp. 109-142
  • Kim, P., Zamora, R., Petrosko, P., Billiar, T., The regulatory role of nitric oxide in apoptosis (2001) Int. Immunopharmacol., 1, pp. 1421-1441
  • Stamler, J., Redox signaling: Nitrosilation and related target interactions of nitric oxide (1994) Cell, 78, pp. 931-936
  • Nathan, C., Xie, Q., Nitric oxide synthases: Roles, tolls and controls (1994) Cell, 78, pp. 915-918
  • Korbelik, M., The role of nitric oxide in the response of solid tumors to photodynamic therapy (1997) Photochem. Photobiol., 65, pp. S55-S56
  • Henderson, B., Sitnik-Busch, T., Vaughan, L., Potentiation of photodynamic therapy antitumor activity in mice by nitric oxide synthase inhibition is fluence rate dependent (1999) Photochem. Photobiol., 70, pp. 64-71
  • Korbelik, M., Parkins, C., Shibuya, H., Cecic, I., Stratford, M., Chaplin, D., Nitric oxide production by tumour tissue: Impact on the response to photodynamic therapy (2000) Br. J. Cancer, 82, pp. 1835-1843
  • Niziolek, M., Korytowski, W., Girotti, A., Nitric oxide inhibition of free radical-mediated lipid peroxidation in photodynamically treated membranes and cells (2003) Free Rad. Biol. Med., 24, pp. 997-1005
  • Gomes, W., Almeida, R., Carvalho, P., Duarte, C., Nitric oxide modulates tumor cell death induced by photodynamic therapy through a cGMP-dependent mechanism (2002) Photochem. Photobiol., 76, pp. 423-430
  • Gupta, S., Ahmad, N., Mukhtar, H., Involvement of nitric oxide during phthalocyanine (Pc4) photodynamic therapy-mediated apoptosis (1998) Cancer Res., 58, pp. 1785-1788
  • Dalbasti, T., Cagli, S., Kiline, E., Oktar, N., Ozsoz, M., Online electrochemical monitoring of nitric oxide during photodynamic therapy (2002) Nitric Oxide, 7, pp. 301-305
  • Eijan, A., Davel, L., Rueda, H., Rozenberg, G., Lustig, E., Jasnis, M., Differential nitric oxide release and sensitivity to injury in different murine mammary tumour cell lines (1998) Int. J Mo. Med., 2, pp. 625-630
  • Galli, S., Davel, L., Jasnis, M., Sales, M., Lustig, E., Eijan, A., Selección de una línea de células tumorales resistente a la citotoxicidad por óxido nítrico (1999) Medicina De Buenos Aires, 59, p. 63. , (5/2)
  • Galli, S., Colombo, L., Vanzuli, S., Daroqui, M., Vidal, M., Jasnis, A., Lustig, E., Eiján, A., Characterization of a fibroblastoid mammary carcinoma cell line (LM2) originated from a mouse adenocarcinoma (2000) Int. J. Oncol., 17, pp. 1259-1265
  • Urtreger, A., Ladeda, V., Puricelli, L., Rivelli, A., Vidal, M., Lustig, E., Bal de Kier Joffe, E., Modulation of fibronectin expression and proteolytic activity associated with the invasive and metastatic phenotype in two new murine mammary tumor cell lines (1997) Int. J. Oncol., 11, pp. 489-496
  • Granger, D., Hidds, J., Perfect, J., Durack, D., Metabolic fate of L-arginine inhalation to microbiostatic capability of murine macrophages (1990) J. Clin. Invest., 85, pp. 264-273
  • Denizot, F., Lang, R., Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability (1986) J. Immunol. Methods, 89, pp. 271-277
  • Bustos, N., Stella, A., Wider, E., Batlle, A., Studies on erythrocytic aminolevulinate dehydratase. I. Its purification and possible therapeutic applications (1980) Int. J. Biochem., 12, pp. 745-750
  • Batlle, A., Wider, E., Stella, A., A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria (1978) Int. J. Biochem., pp. 871-876
  • Bates, J., Baker, M., Guerra, R., Harrison, D., Nitric oxide generation from nitroprusside by vascular tissue (1991) Biochem. Pharmacol., 42, pp. S157-S165
  • Ioannidis, I., Batz, M., Thomas, P., Korth, H.-G., Sustmann, R., de Groot, H., Enhanced release of nitric oxide causes increased cytotoxicity of S-nitroso-N-acetyl-dl-penicillamine and sodium nitroprusside under hypoxic conditions (1996) Biochem. J., 318, pp. 789-795
  • Fraga, C., Onuki, J., Lucesoli, F., Bechara, E., Di Mascio, P., 5-Aminolevulinic acid mediates the in vivo and in vitro formation of 8-hydoxy-2′-deoxyguanosine in DNA (1994) Carcinogenesis, 15, pp. 2241-2244
  • Wolff, D., Naddelman, A., Lubeskie, A., Saks, D., Inhibition of nitric oxide synthase isoforms by porphyrins (1996) Arch. Biochem. Biophys., 333, pp. 27-34
  • Correa García, S., Casas, A., Perotti, C., Batlle, A., Bermudez Moretti, M., Mechanistic studies on 5-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line (2003) Br. J. Cancer, 89, pp. 173-177
  • Fukumura, D., Yuan, F., Endo, M., Jain, K., Role of nitric oxide in tumor microcirculation. Blood flow, vascular permeability, and leukocyte-endothelial interactions (1997) Am. Pathol., 150, pp. 713-725
  • Wood, P., Sansom, S., Butler, A., Stratford, I., Cole, S., Szabo, C., Thiemermann, C., Admas, G., Induction of hypoxia in experimental murine tumors by the nitric oxide synthase inhibitor, NG-nitro-l-arginine (1994) Cancer Res., 54, pp. 6458-6463
  • Beckman, J., Oxidative damage and tryrosine nitration from peroxynitrite (1996) Chem. Res. Toxicol., 9, pp. 836-844
  • Wink, D., Mitchell, J., Chemical biology of nitric oxide: Insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide (1998) Free Radic. Biol. Med., 25, pp. 434-456
  • Rubbo, H., Radi, M., Trujillo, M., Telleri, R., Kalyanaraman, B., Barnes, S., Kirk, M., Freeman, B., Nitric oxide regulation of superoxide and peroxynitrite-dependent lipid peroxidation. Formation of novel nitrogen-containing oxidized lipid derivatives (1994) J. Biol. Chem., 269, pp. 26066-26075
  • Rossig, L., Fichtlscherer, B., Breitschopf, K., Haendeler, J., Zeiher, A., Mulsch, A., Dimmeler, G., Nitric oxide inhibits caspase-3 by S-nitrosation in vivo (1999) J. Biol. Chem., 274, pp. 6823-6826

Citas:

---------- APA ----------
Di Venosa, G., Perotti, C., Fukuda, H., Batlle, A. & Casas, A. (2005) . Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity. Journal of Photochemistry and Photobiology B: Biology, 80(3), 195-202.
http://dx.doi.org/10.1016/j.jphotobiol.2005.05.001
---------- CHICAGO ----------
Di Venosa, G., Perotti, C., Fukuda, H., Batlle, A., Casas, A. "Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity" . Journal of Photochemistry and Photobiology B: Biology 80, no. 3 (2005) : 195-202.
http://dx.doi.org/10.1016/j.jphotobiol.2005.05.001
---------- MLA ----------
Di Venosa, G., Perotti, C., Fukuda, H., Batlle, A., Casas, A. "Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity" . Journal of Photochemistry and Photobiology B: Biology, vol. 80, no. 3, 2005, pp. 195-202.
http://dx.doi.org/10.1016/j.jphotobiol.2005.05.001
---------- VANCOUVER ----------
Di Venosa, G., Perotti, C., Fukuda, H., Batlle, A., Casas, A. Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity. J. Photochem. Photobiol. B Biol. 2005;80(3):195-202.
http://dx.doi.org/10.1016/j.jphotobiol.2005.05.001