Navegar

Colección

Datos Estadísticas

Artículo

Di Venosa, G.; Fukuda, H.; Perotti, C.; Batlle, A.; Casas, A. "A method for separating ALA from ALA derivatives using ionic exchange extraction" (2004) Journal of Photochemistry and Photobiology B: Biology. 75(3):157-163
La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Photodynamic therapy using 5-aminolevulinic acid (ALA)-induced protoporphyrin IX is a recent approach to detect and treat some malignancies. The use of lipophilic derivatives of ALA has been exploited in the last years to enhance ALA penetration. In this paper, we describe the application of the Mauzerall and Granick's method [J. Biol. Chem. 219 (1956) 435] to the quantification of ALA derivatives. We also describe the employment of reusable ion-exchange chromatographic columns for separating mixtures of ALA and ALA derivatives present in biological samples. The relation between 555 nm absorbance and ALA or ALA derivative concentration was linear up to 100 nmol/ml and the limit of detection of ALA and ALA derivatives was 1 nmol per ml. We employed a Dowex 50 X8 hydrogen form resin to separate ALA from the derivatives. Whereas 90 ± 4% of the total ALA was eluted using sodium acetate, only 3-9% of the ALA derivatives was recovered. Only upon exposure of the resin to a high HCl concentration, the ALA derivatives were completely released. We employed this new method for the separation of ALA from ALA derivatives in cells exposed to different ALA compounds. © 2004 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:A method for separating ALA from ALA derivatives using ionic exchange extraction
Autor:Di Venosa, G.; Fukuda, H.; Perotti, C.; Batlle, A.; Casas, A.
Filiación:CIPYP, CONICET and FCEN, University of Buenos Aires, Ciudad Universitaria Pabellon II, 1428 Capital Federal, Argentina
Viamonte 1881 10A, 1056 Buenos Aires, Argentina
Palabras clave:ALA; ALA derivatives; ALA esters; Aminolevulinic acid; Dowex resin; PDT; Photodynamic therapy; acetic acid; amino acid derivative; aminolevulinic acid; hydrochloric acid; hydrogen; resin; animal cell; article; concentration (parameters); controlled study; elution; extraction; ion exchange; light absorption; nonhuman; priority journal; product recovery; separation technique; Aminolevulinic Acid; Animals; Chromatography, Ion Exchange; Mice; Tumor Cells, Cultured
Año:2004
Volumen:75
Número:3
Página de inicio:157
Página de fin:163
DOI: http://dx.doi.org/10.1016/j.jphotobiol.2004.07.001
Título revista:Journal of Photochemistry and Photobiology B: Biology
Título revista abreviado:J. Photochem. Photobiol. B Biol.
ISSN:10111344
CODEN:JPPBE
CAS:acetic acid, 127-08-2, 127-09-3, 64-19-7, 71-50-1; aminolevulinic acid, 106-60-5; hydrochloric acid, 7647-01-0; hydrogen, 12385-13-6, 1333-74-0; Aminolevulinic Acid, 106-60-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10111344_v75_n3_p157_DiVenosa

Referencias:

  • Mauzerall, M., Granick, S., The occurrence and determination of 5-aminolevulinic acid and porphobilinogen in urine (1956) J. Biol. Chem., 219, pp. 435-437
  • Lui, H., Anderson, R., Photodynamic therapy for the treatment of basal cell carcinoma (1992) Arch. Dermatol., 128, pp. 1597-1601
  • Oseroff, A., Photodynamic therapy (1993) Clinical Photomedicine, pp. 387-402. , H. Lim N. Sorter (Eds.) Marcel Dekker New York
  • Baumgartner, R., Huber, R., Schulz, H., Stepp, H., Rick, K., Gamarra, F., Leberig, A., Roth, C., Inhalation of 5-aminolevulinic acid: A new technique for fluorescence detection of early stage lung cancer (1996) J. Photochem. Photobiol. B, 36, pp. 167-174
  • Jichlinski, P., Forrer, M., Mizeret, J., Glanzmann, T., Braichotte, D., van den Bergh, H., Leisinger, H., Clinical evaluation of a method for detecting superficial transitional cell carcinoma of the bladder by light-iduced fluorescence of protoporphyrin IX following topical application of 5-aminolevulinic acid: Preliminary results (1997) Lasers Surg. Med., 20, pp. 402-408
  • Fan, K., Hopper, C., Speight, P., Buonaccorsi, G., Macrobert, A., Bown, S., Photodynamic therapy using 5-aminolevulinic acid for premalignant and malignant lesions of the oral cavity (1996) Cancer, 78, pp. 1374-1383
  • Gossner, L., Stolte, M., Sroka, R., Rick, K., May, A., Hahn, E., Ell, C., Photodynamic ablation of high-grade dysplasia and early cancer in Barrett's esophagus by means of 5-aminolevulinic acid (1998) Gastroenterology, 114, pp. 448-455
  • Wyss, P., Fehr, M., van den Bergh, H., Haller, U., Feasibility of photodynamic endometrial ablation without anesthesia (1998) Int. J. Gynec. Obstet., 60, pp. 287-288
  • Stummer, W., Stocker, S., Wagner, S., Stepp, H., Fritsch, C., Goetz, C., Goetz, A., Reulen, H., Intraoperative detection of malignant glioma by 5-ALA-induced porphyrin fluorescence (1998) Neurosurgery, 42, pp. 518-525
  • Kriegmair, M., Baumgartner, R., Knuechel, R., Stepp, H., Hofstädter F., F., Hofstetter, A., Detection of early bladder cancer by 5-aminolevulinic acid induced porphyrin fluorescence (1996) J. Urol., 155, pp. 105-110
  • Casas, A., Batlle, A., Rational design of 5-aminolevulinic acid derivatives aimed at improving Photodynamic Therapy (2002) Curr Med. Chem. Anti-Cancer Agents, 2, pp. 465-475
  • Perotti, C., Fukuda, H., Di Venosa, G., MacRobert, A., Batlle, A., Casas, A., Porphyrin synthesis from ALA derivatives for photodynamic therapy. In vitro and in vivo studies (2004) Br. J. Cancer, 90, pp. 1660-1665
  • Takeya, H., Preparation of 5-aminolevulinic acid alkyl esters as herbicides (1992) Chem. Abs., 116, pp. 189633m
  • Shriner, R., Fuson, R., Curtin, D., Application of classification tests (1980) The Systematic Identification of Organic Compounds. A Laboratory Manual, , New York: Wiley
  • Werbajh, S., Urtreger, A., Puricelli, L., de Lustig, E., Bal de Kier Joffe, E., Kornblihtt, A., Downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells (1998) FEBS Lett., 440, pp. 277-281

Citas:

---------- APA ----------
Di Venosa, G., Fukuda, H., Perotti, C., Batlle, A. & Casas, A. (2004) . A method for separating ALA from ALA derivatives using ionic exchange extraction. Journal of Photochemistry and Photobiology B: Biology, 75(3), 157-163.
http://dx.doi.org/10.1016/j.jphotobiol.2004.07.001
---------- CHICAGO ----------
Di Venosa, G., Fukuda, H., Perotti, C., Batlle, A., Casas, A. "A method for separating ALA from ALA derivatives using ionic exchange extraction" . Journal of Photochemistry and Photobiology B: Biology 75, no. 3 (2004) : 157-163.
http://dx.doi.org/10.1016/j.jphotobiol.2004.07.001
---------- MLA ----------
Di Venosa, G., Fukuda, H., Perotti, C., Batlle, A., Casas, A. "A method for separating ALA from ALA derivatives using ionic exchange extraction" . Journal of Photochemistry and Photobiology B: Biology, vol. 75, no. 3, 2004, pp. 157-163.
http://dx.doi.org/10.1016/j.jphotobiol.2004.07.001
---------- VANCOUVER ----------
Di Venosa, G., Fukuda, H., Perotti, C., Batlle, A., Casas, A. A method for separating ALA from ALA derivatives using ionic exchange extraction. J. Photochem. Photobiol. B Biol. 2004;75(3):157-163.
http://dx.doi.org/10.1016/j.jphotobiol.2004.07.001