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Lombardo, M.E.; Araujo, L.S.; Del C. Batlle, A.M. "Kinetic studies on the glutamate:glyoxylate transaminase of Euglena gracilis" (1996) Journal of Photochemistry and Photobiology B: Biology. 36(3):241-244
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Abstract:

The kinetic properties of L-glutamate:glyoxylate transminase have been investigated. Glycine formation was linear with both protein concentration and incubation time. L-glutamate was the most effective amino donor. Optimal concentrations for L-glutamate and glyoxylate were 150-200 mM and 10-20 mM, respectively. Initial velocity studies suggested a ping-pong reaction mechanism with a K(m) value of 89.90-66.50 mM for L-glutamate and of 12.50-10.75 mM for glyoxylate. A competitive parabolic substrate inhibition by glyoxylate at concentrations greater than 20 mM was observed. A pure linear non-competitive inhibition between glyoxylate and 4,5-dioxovaleric acid (1.1-2.8 mM) was found. We show here that in vivo different enzymes exist for the transamination of glyoxylate and DOVA.

Registro:

Documento: Artículo
Título:Kinetic studies on the glutamate:glyoxylate transaminase of Euglena gracilis
Autor:Lombardo, M.E.; Araujo, L.S.; Del C. Batlle, A.M.
Filiación:Ctro. de Invest. Sobre P., Ciudad Universitaria Pab. II 2do., Piso - 1428, Buenos Aires, Argentina
Viamonte 1881, 10 A, CP-1056 Buenos Aires, Argentina
Palabras clave:5-aminolevulinic acid biosynthesis; Euglena gracilis; Glutamate:4,5-dioxovalerate trasaminase; Glutamate:glyoxylate transaminase; aminotransferase; glutamic acid; glycine; glyoxylic acid; valeric acid derivative; article; controlled study; enzyme inhibition; enzyme kinetics; enzyme mechanism; enzyme substrate complex; euglena gracilis; nonhuman; priority journal; transamination
Año:1996
Volumen:36
Número:3
Página de inicio:241
Página de fin:244
DOI: http://dx.doi.org/10.1016/S1011-1344(96)07387-3
Título revista:Journal of Photochemistry and Photobiology B: Biology
Título revista abreviado:J. PHOTOCHEM. PHOTOBIOL. B BIOL.
ISSN:10111344
CODEN:JPPBE
CAS:aminotransferase, 9031-66-7; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; glycine, 56-40-6, 6000-43-7, 6000-44-8; glyoxylic acid, 298-12-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10111344_v36_n3_p241_Lombardo

Referencias:

  • Collins, N., Merrett, M.J., The localization of glycollate-pathway enzymes, Euglena (1975) Biochem. J., 148, pp. 321-328
  • Noguchi, T., Mori, R., Biosynthesis of porphyrin precursors in mammals. Identity of alanine: ,δ-dioxovalerate aminotransferase with alanine: Glyoxylate aminotransferase (1981) J. Biol. Chem., 256, pp. 10335-10339
  • Foley, T., Beale, S.I., δ-Aminolevulinic acid formation from ,δ-dioxovaleric acid in extracts of Euglena gracilis (1982) Plant Physiol., 70, pp. 1495-1502
  • Shioi, Y., Nagamine, M., Sasa, T., Purification and properties of L-alanine:4,5-dioxovalerate aminotransferase from Chlorella regularis (1984) Arch. Biochem. Biophys., 234, pp. 117-124
  • Lombardo, M.E., Araujo, L.S., Juknat, A.A., Del C. Batlle, A.M., Glutmate:4,5-dioxovaleric acid transaminase from Euglena gracilis. Kinetic studies (1989) Eur. J. Biochem., 182, pp. 657-660
  • Varticovski, L., Kushner, J.P., Burnham, B.F., Biosynthesis of porphyrin precursors. Purification and characterization of mammaliam L-alanine: ,δ-dioxovaleric acid aminotransferase (1980) J. Biol. Chem., 255, pp. 3742-3747
  • Lombardo, M.E., Araujo, L.S., Juknat, A.A., Del C. Batlle, A.M., Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis (1988) Comp. Biochem. Physiol., 91 B, pp. 279-284
  • Lombardo, M.E., Araujo, L.S., Branca, A., Del C. Batlle, A.M., A method for estimating glycine in the presence of excess glutamate with o-phthaldialdehyde (1990) Z. Naturforsch., 45 C, pp. 911-914
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Polin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275
  • Kah, A., Dörnemamm, D., Senger, H., Isolation and purification to apparent homogeneity of 4,5-dioxovalerate aminotransferase from Scenedesmus obliquus mutant C-2A′ (1988) Z. Naturforsch., 43 C, pp. 563-571

Citas:

---------- APA ----------
Lombardo, M.E., Araujo, L.S. & Del C. Batlle, A.M. (1996) . Kinetic studies on the glutamate:glyoxylate transaminase of Euglena gracilis. Journal of Photochemistry and Photobiology B: Biology, 36(3), 241-244.
http://dx.doi.org/10.1016/S1011-1344(96)07387-3
---------- CHICAGO ----------
Lombardo, M.E., Araujo, L.S., Del C. Batlle, A.M. "Kinetic studies on the glutamate:glyoxylate transaminase of Euglena gracilis" . Journal of Photochemistry and Photobiology B: Biology 36, no. 3 (1996) : 241-244.
http://dx.doi.org/10.1016/S1011-1344(96)07387-3
---------- MLA ----------
Lombardo, M.E., Araujo, L.S., Del C. Batlle, A.M. "Kinetic studies on the glutamate:glyoxylate transaminase of Euglena gracilis" . Journal of Photochemistry and Photobiology B: Biology, vol. 36, no. 3, 1996, pp. 241-244.
http://dx.doi.org/10.1016/S1011-1344(96)07387-3
---------- VANCOUVER ----------
Lombardo, M.E., Araujo, L.S., Del C. Batlle, A.M. Kinetic studies on the glutamate:glyoxylate transaminase of Euglena gracilis. J. PHOTOCHEM. PHOTOBIOL. B BIOL. 1996;36(3):241-244.
http://dx.doi.org/10.1016/S1011-1344(96)07387-3