Artículo

Vignoni, M.; Erra-Balsells, R.; Epe, B.; Cabrerizo, F.M. "Intra- and extra-cellular DNA damage by harmine and 9-methyl-harmine" (2014) Journal of Photochemistry and Photobiology B: Biology. 132:66-71
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Abstract:

It is known that β-carbolines are able to produce photosensitized damage in cell-free DNA, but there is little information on their effects on cellular DNA. Therefore, we have analyzed the DNA damage produced by harmine and 9-methyl-harmine under UVA irradiation in V79 cells, together with the associated generation of micronuclei and photocytotoxicity. The results indicate that the most frequent photoproducts generated in the cellular DNA are modified purines such as 8-oxo-7,8-dihydroguanine. Only relatively few single-strand breaks were observed. CPDs were absent, although they were generated in cell-free DNA irradiated under the same conditions. The overall extent of DNA damage in the cells was considerably smaller than the one observed in cell free DNA. The generation of cellular DNA damage was associated with a significant generation of micronuclei and decreased cell proliferation. The data indicate that β-carbolines act as photosensitizers in mammalian cells. The spectrum of DNA modification, and therefore the mechanism of DNA damage generation, differs considerably from that observed with cell-free DNA. © 2014 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Intra- and extra-cellular DNA damage by harmine and 9-methyl-harmine
Autor:Vignoni, M.; Erra-Balsells, R.; Epe, B.; Cabrerizo, F.M.
Filiación:Instituto de Investigaciones Biotecnológicas, Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM), Intendente Marino, CC 164 (B7130IWA), Chascomús, Argentina
Institute of Pharmacy and Biochemistry, University of Mainz, Staudingerweg 5, Mainz, Germany
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellón 2, 3p, 1428 Buenos Aires, Argentina
Palabras clave:Cyclobutane pyrimidine photodimers; Micronuclei; Photosensitization; Phototoxicity; β-carbolines; 8 hydroxyguanine; 9 methyl harmine; beta carboline derivative; cell DNA; harmine; unclassified drug; 8-hydroxyguanine; DNA; guanine; harmine; absorption; animal cell; article; cell counting; cell proliferation; controlled study; cytotoxicity; DNA damage; DNA modification; gel electrophoresis; micronucleus; nonhuman; pH; phototoxicity; priority journal; ultraviolet A radiation; analogs and derivatives; animal; chemistry; CHO cell line; Cricetulus; DNA damage; drug effects; hamster; radiation response; toxicity; ultraviolet radiation; Animals; CHO Cells; Cricetinae; Cricetulus; DNA; DNA Damage; Guanine; Harmine; Hydrogen-Ion Concentration; Ultraviolet Rays
Año:2014
Volumen:132
Página de inicio:66
Página de fin:71
DOI: http://dx.doi.org/10.1016/j.jphotobiol.2014.01.020
Título revista:Journal of Photochemistry and Photobiology B: Biology
Título revista abreviado:J. Photochem. Photobiol. B Biol.
ISSN:10111344
CODEN:JPPBE
CAS:8 hydroxyguanine, 5614-64-2; harmine, 343-27-1, 442-51-3; DNA, 9007-49-2; guanine, 69257-39-2, 73-40-5; 8-hydroxyguanine; DNA; Guanine; Harmine
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10111344_v132_n_p66_Vignoni

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Citas:

---------- APA ----------
Vignoni, M., Erra-Balsells, R., Epe, B. & Cabrerizo, F.M. (2014) . Intra- and extra-cellular DNA damage by harmine and 9-methyl-harmine. Journal of Photochemistry and Photobiology B: Biology, 132, 66-71.
http://dx.doi.org/10.1016/j.jphotobiol.2014.01.020
---------- CHICAGO ----------
Vignoni, M., Erra-Balsells, R., Epe, B., Cabrerizo, F.M. "Intra- and extra-cellular DNA damage by harmine and 9-methyl-harmine" . Journal of Photochemistry and Photobiology B: Biology 132 (2014) : 66-71.
http://dx.doi.org/10.1016/j.jphotobiol.2014.01.020
---------- MLA ----------
Vignoni, M., Erra-Balsells, R., Epe, B., Cabrerizo, F.M. "Intra- and extra-cellular DNA damage by harmine and 9-methyl-harmine" . Journal of Photochemistry and Photobiology B: Biology, vol. 132, 2014, pp. 66-71.
http://dx.doi.org/10.1016/j.jphotobiol.2014.01.020
---------- VANCOUVER ----------
Vignoni, M., Erra-Balsells, R., Epe, B., Cabrerizo, F.M. Intra- and extra-cellular DNA damage by harmine and 9-methyl-harmine. J. Photochem. Photobiol. B Biol. 2014;132:66-71.
http://dx.doi.org/10.1016/j.jphotobiol.2014.01.020