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Abstract:

Heparin, a highly sulfated polysaccharide used as an antithrombotic and anticoagulant, inhibits proliferation of several cell types. We have investigated the effect of heparin and chemically modified heparins on the growth of a cell culture of a murine mammary adenocarcinoma (M3). We found that heparin inhibited the proliferation of M3 cells growing either with or without 2% fetal calf serum (FCS) in a dose-dependent and reversible fashion. Several heparins with different anticoagulant properties showed a similar antiproliferative effect. Histological assays showed that heparin was internalized and appeared in cytoplasmic vesicules. O-desulfated. O/N-desul- fated N-acetylated and N-desulfated heparins lost their antiproliferative activity, while N-desulfated N-acetylated heparin significantly inhibited cell proliferation with or without FCS. The finding of an antiproliferative action of N-desulfated N-acetylated heparin which does not show anticoagulant activity suggests a possible therapeutic role for this compound as an antineoplastic drug. © 1994 S. Karger AG, Basel.

Registro:

Documento: Artículo
Título:Growth inhibition in vitro of murine mammary adenocarcinoma cells by heparin and chemically modified heparins
Autor:Bertolesi, G.E.; De Cidre, L.L.; Eiján, A.M.
Filiación:Area de Investigaciones, Institute de Oncología ‘A.H. Roffo’, Universidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio de Histología Animal, Departamento de Biología, Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Growth; Heparin; Heparinoid; Inhibition; Metastasis; Murine adenocarcinoma; heparin; heparin derivative; animal cell; animal experiment; animal model; article; breast carcinoma; controlled study; growth inhibition; mouse; nonhuman; priority journal; Adenocarcinoma; Animal; Cell Division; Comparative Study; Cytoplasmic Granules; Heparin; Kinetics; Mammary Neoplasms, Experimental; Mice; Mice, Inbred BALB C; Support, Non-U.S. Gov't; Time Factors; Tumor Cells, Cultured
Año:1994
Volumen:15
Número:5
Página de inicio:275
Página de fin:283
DOI: http://dx.doi.org/10.1159/000217902
Título revista:Tumor Biology
Título revista abreviado:Tumor Biol.
ISSN:10104283
CAS:heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; Heparin, 9005-49-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10104283_v15_n5_p275_Bertolesi

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Citas:

---------- APA ----------
Bertolesi, G.E., De Cidre, L.L. & Eiján, A.M. (1994) . Growth inhibition in vitro of murine mammary adenocarcinoma cells by heparin and chemically modified heparins. Tumor Biology, 15(5), 275-283.
http://dx.doi.org/10.1159/000217902
---------- CHICAGO ----------
Bertolesi, G.E., De Cidre, L.L., Eiján, A.M. "Growth inhibition in vitro of murine mammary adenocarcinoma cells by heparin and chemically modified heparins" . Tumor Biology 15, no. 5 (1994) : 275-283.
http://dx.doi.org/10.1159/000217902
---------- MLA ----------
Bertolesi, G.E., De Cidre, L.L., Eiján, A.M. "Growth inhibition in vitro of murine mammary adenocarcinoma cells by heparin and chemically modified heparins" . Tumor Biology, vol. 15, no. 5, 1994, pp. 275-283.
http://dx.doi.org/10.1159/000217902
---------- VANCOUVER ----------
Bertolesi, G.E., De Cidre, L.L., Eiján, A.M. Growth inhibition in vitro of murine mammary adenocarcinoma cells by heparin and chemically modified heparins. Tumor Biol. 1994;15(5):275-283.
http://dx.doi.org/10.1159/000217902