Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Rat protein DE is an androgen-dependent cysteine-rich secretory protein (CRISP) synthesized by proximal epididymal regions. DE, also known as CRISP-1, is localized on the equatorial segment of acrosome-reacted spermatozoa and participates in gamete fusion through binding to egg complementary sites. Immunization of rats with DE inhibits fertility and sperm fusion ability, suggesting that DE represents a good epididymal contraceptive target. Recombinant DE fragments and synthetic peptides revealed that DE binds to the egg via a 12-amino acid region of an evolutionarily conserved motif, Signature 2 (S2). The ability of other CRISP to bind to the rat egg was correlated with their S2 amino acid sequences. Although testicular protein Tpx-1 (CRISP-2) was capable of binding to rodent eggs, human epididymal AEG-related protein (ARP) and helothermine (from lizard saliva) were not. The S2 region presented only two substitutions in Tpx-1 and four in ARP and helothermine, compared with the DE S2, suggesting that this amino acid sequence was relevant for egg interaction. Studies with Tpx-1 and anti-Tpx-1 revealed the participation of this protein in gamete fusion through binding to complementary sites in the egg. In competition studies, DE reduced binding of Tpx-1 dose-dependently, indicating that both CRISP share the egg complementary sites. That anti-DE and anti-Tpx-1 inhibit sperm-egg fusion while recognizing only the corresponding proteins, suggests functional cooperation between these homologous CRISP to ensure fertilization success. These results increase our understanding of the molecular mechanisms of gamete fusion and contribute to the development of new and safer fertility regulating methods. © 2007 Asian Journal of Andrology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences.

Registro:

Documento: Artículo
Título:Participation of epididymal cysteine-rich secretory proteins in sperm-egg fusion and their potential use for male fertility regulation
Autor:Cohen, D.J.; Da Ros, V.G.; Busso, D.; Ellerman, D.A.; Maldera, J.A.; Goldweic, N.; Cuasnicú, P.S.
Filiación:Institute of Biology and Experimental Medicine, Vuelta de Obligado 2490, Buenos Aires 1428, Argentina
Palabras clave:Contraception; Cysteine-rich secretory protein; Epididymis; Gamete fusion; Sperm; amino acid; androgen; cell protein; contraceptive agent; cysteine; egg protein; epididymal secretory protein; synthetic peptide; acrosome reaction; amino acid sequence; amino acid substitution; cell fusion; drug targeting; egg; epididymis; fertilization; gamete; genetic conservation; lizard; male fertility; molecular mechanics; nonhuman; protein binding; protein localization; protein motif; protein synthesis; regulatory mechanism; review; saliva; sequence homology; spermatozoon; Animals; Cell Fusion; Epididymis; Female; Germ Cells; Glycoproteins; Humans; Male; Membrane Glycoproteins; Ovum; Rats; Sperm Capacitation; Sperm-Ovum Interactions; Spermatozoa
Año:2007
Volumen:9
Número:4
Página de inicio:528
Página de fin:532
DOI: http://dx.doi.org/10.1111/j.1745-7262.2007.00283.x
Título revista:Asian Journal of Andrology
Título revista abreviado:Asian J. Androl.
ISSN:1008682X
CODEN:ASJAF
CAS:amino acid, 65072-01-7; cysteine, 4371-52-2, 52-89-1, 52-90-4; CRISP1 protein, human; CRISP2 protein, human; Glycoproteins; Membrane Glycoproteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1008682X_v9_n4_p528_Cohen

Referencias:

  • Cameo, M.S., Blaquier, J.A., Androgen-controlled specific proteins in rat epididymis (1976) J Endocrinol, 69, pp. 317-324
  • Garberi, J.C., Kohane, A.C., Cameo, M.S., Blaquier, J.A., Isolation and characterization of specific rat epididymal proteins (1979) Mol Cell Endocrinol, 13, pp. 73-82
  • Garberi, J.C., Fontana, J.D., Blaquier, J.A., Carbohydrate composition of specific rat epididymal protein (1982) Int J Androl, 5, pp. 619-626
  • Kohane, A.C., Cameo, M.S., Piñeiro, L., Garberi, J.C., Blaquier, J.A., Distribution and site of production of specific proteins in the rat epididymis (1980) Biol Reprod, 23, pp. 181-187
  • Kohane, A.C., Gonzalez Echeverria, F., Piñeiro, L., Blaquier, J.A., Interaction of proteins of epididymal origin with spermatozoa (1980) Biol Reprod, 23, pp. 737-742
  • Hardy, D.M., Huang, T.T., Driscoll, W.J., Tung, K.S.K., Wild, G.C., Purification and characterization of the primary acrosomal autoantigen of guinea pig epididymal spermatozoa (1998) Biol Reprod, 38, pp. 423-437
  • Kasahara, M., Gutknecht, J., Brew, K., Spurr, N., Goodfellow, P.N., Cloning and mapping of a testis-specific gene with sequence similarity to a sperm-coating glycoprotein gene (1989) Genomics, 5, pp. 527-534
  • Haendler, B., Kratzschmar, J., Theuring, F., Schleuning, W.D., Transcripts for cysteine-rich secretory protein-1 (CRISP-1; DE/AEG) and the novel related CRISP-3 are expressed under androgen control in the mouse salivary gland (1993) Endocrinology, 133, pp. 192-198
  • Kjeldsen, L., Cowland, J.B., Johnsen, A.H., Borregaard, N., SGP28, a novel matrix glycoprotein in specific granules of human neutrophils with similarity to a human testis-specific gene product and a rodent sperm-coating glycoprotein (1996) FEBS Lett, 380, pp. 246-250
  • Udby, L., Bjartell, A., Malm, J., Egesten, A., Lundwall, A., Cowland, J.B., Characterization and localization of cysteine-rich secretory protein 3 (CRISP-3) in the human male reproductive tract (2005) J Androl, 26, pp. 333-342
  • Jalkanen, J., Huhtaniemi, I., Poutanen, M., Mouse cysteine-rich secretory protein 4 (CRISP4): A member of the crisp family exclusively expressed in the epididymis in an androgen-dependent manner (2005) Biol Reprod, 72, pp. 1268-1274
  • Nolan, M.A., Wu, L., Bang, H.J., Jelinsky, S.A., Roberts, K.P., Turner, T.T., Identification of rat cysteine-rich secretory protein 4 (Crisp4) as the ortholog to human CRISP1 and mouse Crisp4 (2006) Biol Reprod, 74, pp. 984-991
  • Guo, M., Teng, M., Niu, L., Liu, Q., Huang, Q., Hao, Q., Crystal structure of the cysteine-rich secretory protein stecrisp reveals that the cystein e-rich domain has a K+ channel inhibitor-like fold (2005) J Biol Chem, 280, pp. 12405-12412
  • Rochwerger, L., Cuasnicú, P.S., Redistribution of a rat sperm epididymal glycoprotein after in vivo and in vitro capacitation (1992) Mol Reprod Dev, 31, pp. 34-41
  • Bedford, J.M., Moore, H.D., Franklin, L.E., Significance of the equatorial segment of the acrosome of the spermatozoon in Eutherian mammals (1979) Exp Cell Res, 119, pp. 119-126
  • Yanagimachi, R., Mammalian fertilization (1994) The Physiology of Reproduction, pp. 189-317. , In: Knobil E, Neill JD, editors New York: Raven Press
  • Rochwerger, L., Cohen, D.J., Cuasnicú, P.S., Mammalian sperm-egg fusion: The rat egg has complementary sites for a sperm protein that mediates gamete fusion (1992) Dev Biol, 153, pp. 83-90
  • Cohen, D.J., Rochwerger, L., Ellerman, D.A., Morgenfeld, M., Busso, D., Cuasnicú, P.S., Relationship between the association of rat epididymal protein DE with spermatozoa and the behavior and function of the protein (2000) Mol Reprod Dev, 56, pp. 180-188
  • Cuasnicú, P.S., Conesa, D., Rochwerger, L., Potential contraceptive use of an epididymal protein that participates in fertilization (1990) Gamete Interaction. Prospects for Immunocontraception, pp. 143-153. , In: Alexander NJ, Griffin D, Spieler JM, Waites GM, editors. New York: Wiley-Liss
  • Perez Martinez, S., Conesa, D., Cuasnicú, P.S., Potential contraceptive use of epididymal proteins: Evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition (1995) J Reprod Immunol, 29, pp. 31-45
  • Ellerman, D.A., Brantua, V.S., Martinez, S.P., Cohen, D.J., Conesa, D., Cuasnicú, P.S., Potential contraceptive use of epididymal proteins: Immunization of male rats with epididymal protein DE inhibits sperm fusion ability (1998) Biol Reprod, 59, pp. 1029-1036
  • Mizuki, N., Kasahara, M., Mouse submandibular glands express an androgen-regulated transcript encoding an acidic epididymal glycoprotein-like molecule (1992) Mol Cell Endocrinol, 89, pp. 25-32
  • Cohen, D.J., Ellerman, D.A., Cuasnicú, P.S., Mammalian sperm-egg fusion: Evidence that epididymal protein DE plays a role in mouse gamete fusion (2000) Biol Reprod, 63, pp. 462-468
  • Hayashi, M., Fujimoto, S., Takano, H., Ushiki, T., Abe, K., Ishikura, H., Characterization of a human glycoprotein with potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1) (1996) Genomics, 32, pp. 367-374
  • Kratzschmar, J., Haendler, B., Eberspaecher, U., Roosterman, D., Donner, P., Schleuning, W.D., The human cysteine-rich secretory protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3 (1996), 236, pp. 827-836; Cohen, D.J., Ellerman, D.A., Busso, D., Morgenfeld, M., Piazza, A., Hayashi, M., Evidence that human epididymal protein ARP plays a role in gamete fusion through complementary sites on the surface of the human egg (2001) Biol Reprod, 65, pp. 1000-1005
  • Ellerman, D.A., Da Ros, V.G., Cohen, D.J., Busso, D., Morgenfeld, M.M., Cuasnicú, P.S., Expression and structure-function analysis of DE, a sperm cysteine-rich secretory protein that mediates gamete fusion (2002) Biol Reprod, 67, pp. 1225-1231
  • Ellerman, D.A., Cohen, D.J., Da Ros, V.G., Morgenfeld, M.M., Busso, D., Cuasnicú, P.S., Sperm protein "DE" mediates gamete fusion through an evolutionarily conserved site of the CRISP family (2006) Dev Biol, 297, pp. 228-237
  • Yamazaki, Y., Morita, T., Structure and function of snake venom cysteine-rich secretory proteins (2004) Toxicon, 44, pp. 227-231
  • Gibbs, G.M., Scanlon, M.J., Swarbrick, J., Curtis, S., Gallant, E., Dulhunty, A.F., The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling (2006) J Biol Chem, 281, pp. 4156-4163
  • Roberts, K.P., Wamstad, J.A., Ensrud, K.M., Hamilton, D.W., Inhibition of capacitation-associated tyrosine phosphorylation signaling in rat sperm by epididymal protein crisp-1 (2003) Biol Reprod, 69, pp. 572-581
  • Visconti, P.E., Westbrook, V.A., Chertihin, O., Demarco, I., Sleight, S., Diekman, A.B., Novel signaling pathways involved in sperm acquisition of fertilizing capacity (2002) J Reprod Immunol, 53, pp. 133-150
  • Morrissette, J., Kratzschmar, J., Haendler, B., El-Hayek, R., Mochca-Morales, J., Martin, B.M., Primary structure and properties of helothermine, a peptide that blocks ryanodine receptors (1995) Biophys J, 68, pp. 2280-2288
  • Busso, D., Goldweic, N.M., Hayashi, M., Kasahara, M., Cuasnicú, P.S., Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion (2007) Biol Reprod, 76, pp. 701-708

Citas:

---------- APA ----------
Cohen, D.J., Da Ros, V.G., Busso, D., Ellerman, D.A., Maldera, J.A., Goldweic, N. & Cuasnicú, P.S. (2007) . Participation of epididymal cysteine-rich secretory proteins in sperm-egg fusion and their potential use for male fertility regulation. Asian Journal of Andrology, 9(4), 528-532.
http://dx.doi.org/10.1111/j.1745-7262.2007.00283.x
---------- CHICAGO ----------
Cohen, D.J., Da Ros, V.G., Busso, D., Ellerman, D.A., Maldera, J.A., Goldweic, N., et al. "Participation of epididymal cysteine-rich secretory proteins in sperm-egg fusion and their potential use for male fertility regulation" . Asian Journal of Andrology 9, no. 4 (2007) : 528-532.
http://dx.doi.org/10.1111/j.1745-7262.2007.00283.x
---------- MLA ----------
Cohen, D.J., Da Ros, V.G., Busso, D., Ellerman, D.A., Maldera, J.A., Goldweic, N., et al. "Participation of epididymal cysteine-rich secretory proteins in sperm-egg fusion and their potential use for male fertility regulation" . Asian Journal of Andrology, vol. 9, no. 4, 2007, pp. 528-532.
http://dx.doi.org/10.1111/j.1745-7262.2007.00283.x
---------- VANCOUVER ----------
Cohen, D.J., Da Ros, V.G., Busso, D., Ellerman, D.A., Maldera, J.A., Goldweic, N., et al. Participation of epididymal cysteine-rich secretory proteins in sperm-egg fusion and their potential use for male fertility regulation. Asian J. Androl. 2007;9(4):528-532.
http://dx.doi.org/10.1111/j.1745-7262.2007.00283.x