Artículo

Paz, R.C.; Rocco, R.A.; Jiménez-Bremont, J.F.; Rodríguez-Kessler, M.; Becerra-Flora, A.; Menéndez, A.B.; Ruíz, O.A. "Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress" (2014) Plant Physiology and Biochemistry. 82:279-288
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Abstract:

Soil alkalinity is one of the most serious agricultural problems limiting crop yields. The legume Lotus tenuis is an important forage acknowledged by its ability to naturally grow in alkaline soils. To gain insight into the molecular responses that are activated by alkalinity in L.tenuis plants, subtractive cDNA libraries were generated from leaves and roots of these plants. Total RNAs of non-stressed plants (pH 5.8; E.C. 1.2), and plants stressed by the addition of 10mM of NaHCO3 (pH 9.0; E.C. 1.9), were used as source of the driver and the tester samples, respectively. RNA samples were collected after 14 and 28 days of treatment. A total of 158 unigenes from leaves and 92 unigenes from roots were obtained and classified into 11 functional categories. Unigenes from these categories (4 for leaves and 8 for roots), that were related with nutrient metabolism and oxidative stress relief were selected, and their differential expression analyzed by qRT-PCR. These genes were found to be differentially expressed in a time dependent manner in L.tenuis during the alkaline stress application. Data generated from this study will contribute to the understanding of the general molecular mechanisms associated to plant tolerance under long-term alkaline stress in plants. © 2014 Elsevier Masson SAS.

Registro:

Documento: Artículo
Título:Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress
Autor:Paz, R.C.; Rocco, R.A.; Jiménez-Bremont, J.F.; Rodríguez-Kessler, M.; Becerra-Flora, A.; Menéndez, A.B.; Ruíz, O.A.
Filiación:Grupo INTERBIODES (Interacciones Biológicas del Desierto/Biological Interactions of Desert), CIGEOBIO (FCEFyN, UNSJ/CONICET), Dpto. de Biología, Av. Ignacio de la Roza 590 (Oeste), J5402DCS Rivadavia, San Juan, Argentina
Unidad de Biotecnología 1, IIB-IINTECH/UNSAM-CONICET, Chascomús, Buenos Aires, Argentina
Instituto Potosino de Investigación Científica y Tecnológica (IPICyT), Camino a la Presa de San José No. 2055, Lomas 4a Sección, CP 78216 San Luis Potosí, SLP, Mexico
Facultad de Ciencias, Universidad Autónoma de San Luis Potosí, Zona Universitaria, Av. Salvador Nava s/n, C.P. 78290 San Luis Potosí, SLP, Mexico
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
PROPLAME-PRHIDEB (CONICET), Argentina
Palabras clave:Adaptive response; Alkaline stress; Long-term response; Lotus tenuis; Root and shoot tissues; Stress induced transcripts; Suppressive subtractive hybridization; antacid agent; drug effects; gene expression regulation; genetics; Lotus; plant; plant root; subtractive hybridization; Antacids; Gene Expression Regulation, Plant; Lotus; Plant Roots; Plant Shoots; Subtractive Hybridization Techniques
Año:2014
Volumen:82
Página de inicio:279
Página de fin:288
DOI: http://dx.doi.org/10.1016/j.plaphy.2014.06.009
Título revista:Plant Physiology and Biochemistry
Título revista abreviado:Plant Physiol. Biochem.
ISSN:09819428
CODEN:PPBIE
CAS:Antacids
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09819428_v82_n_p279_Paz

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Citas:

---------- APA ----------
Paz, R.C., Rocco, R.A., Jiménez-Bremont, J.F., Rodríguez-Kessler, M., Becerra-Flora, A., Menéndez, A.B. & Ruíz, O.A. (2014) . Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress. Plant Physiology and Biochemistry, 82, 279-288.
http://dx.doi.org/10.1016/j.plaphy.2014.06.009
---------- CHICAGO ----------
Paz, R.C., Rocco, R.A., Jiménez-Bremont, J.F., Rodríguez-Kessler, M., Becerra-Flora, A., Menéndez, A.B., et al. "Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress" . Plant Physiology and Biochemistry 82 (2014) : 279-288.
http://dx.doi.org/10.1016/j.plaphy.2014.06.009
---------- MLA ----------
Paz, R.C., Rocco, R.A., Jiménez-Bremont, J.F., Rodríguez-Kessler, M., Becerra-Flora, A., Menéndez, A.B., et al. "Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress" . Plant Physiology and Biochemistry, vol. 82, 2014, pp. 279-288.
http://dx.doi.org/10.1016/j.plaphy.2014.06.009
---------- VANCOUVER ----------
Paz, R.C., Rocco, R.A., Jiménez-Bremont, J.F., Rodríguez-Kessler, M., Becerra-Flora, A., Menéndez, A.B., et al. Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress. Plant Physiol. Biochem. 2014;82:279-288.
http://dx.doi.org/10.1016/j.plaphy.2014.06.009