Abstract:
Transgenic Nicotiana tabacum plants expressing the TGBp1 movement protein of potato virus X (PVX) were studied to investigate the effects caused by this protein on plant physiology and development. TGBp1 caused consistent reductions of size and weight in different organs of these plants; however shoot-to-root ratios were similar to those of control plants. Transgenic seedlings showed smaller root meristems and calli derived from TGBp1 leaves grew at a slower rate through successive subcultures. Microscopic observations of TGBp1 plants revealed flattened chloroplasts containing plastoglobuli-like bodies. Further analyses showed a considerable reduction in photosynthetic rate, lower starch levels in leaves and roots, higher nitrate accumulation in leaves and induction of pathogenesis-related (PR) protein genes. Since these changes were not observed when other PVX sequences were expressed in tobacco, we postulate that TGBp1 is an important symptom contributor in PVX infections. © 2004 Elsevier SAS. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | Over-expression of potato virus X TGBp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations |
Autor: | Kobayashi, K.; Sarrobert, C.; Ares, X.; Rivero, M.M.; Maldonado, S.; Robaglia, C.; Mentaberry, A. |
Filiación: | Inst. Invest. Ing. Genet. Y Biol. M., Fac. de Ciencias Exactas Y Naturales, Vuelta Obligado 2490, 1428 Buenos A., Argentina Lab. du Metabolisme Carbone, Dept. d'Ecophysiologie Veg. M., Ctr. Cadarache, CEA, 13108 S.-P., France Fac. de Ciencias Exactas Y Naturales, Ciudad Universitaria, 1428 Buenos Aires, Argentina, Argentina
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Palabras clave: | Chloroplast; Movement protein; Physiology; Potexvirus; Tobacco; chlorophyll; primer DNA; virus protein; article; gene expression regulation; genetics; growth, development and aging; metabolism; photosynthesis; physiology; polymerase chain reaction; Potexvirus; tobacco; transgenic plant; virology; Chlorophyll; DNA Primers; Gene Expression Regulation, Viral; Photosynthesis; Plants, Genetically Modified; Polymerase Chain Reaction; Potexvirus; Tobacco; Viral Proteins; Nicotiana; Nicotiana obtusifolia; Nicotiana tabacum; Potato virus X; Potexvirus; Solanum tuberosum |
Año: | 2004
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Volumen: | 42
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Número: | 9
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Página de inicio: | 731
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Página de fin: | 738
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DOI: |
http://dx.doi.org/10.1016/j.plaphy.2004.07.010 |
Título revista: | Plant Physiology and Biochemistry
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Título revista abreviado: | Plant Physiol. Biochem.
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ISSN: | 09819428
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CODEN: | PPBIE
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CAS: | chlorophyll, 1406-65-1, 15611-43-5; Chlorophyll, 1406-65-1; DNA Primers; Viral Proteins
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09819428_v42_n9_p731_Kobayashi |
Referencias:
- Angell, S.M., Davies, C., Baulcombe, D.C., Cell-to-cell movement of potato virus X is associated with a change in the size-exclusion limit of plasmodesmata in trichome cells of Nicotiana clevelandii (1996) Virology, 216, pp. 197-201
- Ares, X., Calamante, G., Cabra, S., Lodge, J., Hemenway, P., Beachy, R.N., Transgenic plants expressing potato virus X ORF2 protein (p24) are resistant to tobacco mosaic virus and Ob tobamoviruses (1998) J. Virol., 72, pp. 731-738
- Becker, F., Buschfeld, E., Schell, J., Bachmair, A., Altered response to viral infection by tobacco plants perturbed in ubiquitin system (1993) Plant J., 3, pp. 875-881
- Bleeker, A.B., Patterson, S.E., Last exit: Senescence, abscission, and meristem arrest in Arabidopsis (1997) Plant Cell, 9, pp. 1169-1179
- Castaldo, D.A., Haroon, M., Schrader, L.E., Youngs, V.L., Rapid colorimetric determination of nitrate in plant tissue by titration of salicylic acid (1975) Commun. Soil Sci. Plant Anal., 6, pp. 71-80
- Coic, E.C., Lesaint, C., Comment assurer une bonne nutrition en eau et éléments minéraux en horticulture (1971) Hortic. Fr., 8, pp. 11-14
- Davies, C., Hills, G., Baulcombe, D.C., Sub-cellular localization of the 25-kDa protein encoded in the triple gene block of potato virus X (1993) Virology, 197, pp. 166-175
- Dietrich, R.A., Delaney, T.P., Uknes, S.J., Ward, E.R., Ryals, J., Dangl, J.L., Arabidopsis mutants simulating disease resistance response (1994) Cell, 77, pp. 565-574
- Foster, T.M., Lough, T.J., Emerson, S.J., Lee, R.H., Bowman, J.L., Forster, R.L.S., Lucas, W.J., A surveillance system regulates selective entry of RNA into the shoot apex (2002) Plant Cell, 14, pp. 1497-1508
- Hanfrey, C., Fife, M., Buchanan-Wollaston, V., Leaf senescence in Brassica napus: Expression of genes encoding pathogenesis-related proteins (1996) Plant Mol. Biol., 30, pp. 597-609
- Herbers, K., Meuwly, P., Metraux, J.P., Sonnewald, U., Salicylic acid-independent induction of pathogenesis-related protein transcripts by sugars is dependent on leaf developmental stage (1996) FEBS Lett., 397, pp. 239-244
- Herbers, K., Tacke, E., Hazirezaei, M., Krause, K.P., Melzer, M., Rohde, W., Expression of luteoviral movement protein in transgenic plants leads to carbohydrate accumulation and reduced photosynthetic capacity in source leaves (1997) Plant J., 12, pp. 1045-1056
- Hofius, D., Herbers, K., Melzer, M., Omid, A., Tacke, E., Wolf, S., Sonnewald, U., Evidence for expression level-dependent modulation of carbohydrate status and viral resistance by the potato leaf roll virus movement protein in transgenic tobacco plants (2001) Plant J., 28, pp. 529-543
- Huisman, M.J., Linthorst, H.J.M., Bol, J.F., Cornelissen, B.J.C., The complete nucleotide sequence of potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses (1988) J. Gen. Virol., 69, pp. 1789-1798
- Kalinina, N.O., Fedorkin, O.N., Samuilova, O.V., Maiss, E., Korpela, T., Morozov, S., Expression and biochemical analyses of the recombinant potato virus X 25K movement protein (1996) FEBS Lett., 397, pp. 75-78
- Kessler, F., Schnell, D., Blobel, G., Identification of proteins associated with plastoglobules isolated from pea (Pisum sativum L.) chloroplasts (1999) Planta, 208, pp. 107-113
- Kobayashi, K., Cabral, S., Calamante, G., Maldonado, S., Mentaberry, A., Transgenic tobacco plants expressing potato virus X ORF3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus (2001) Mol. Plant-Microbe Interact., 11, pp. 1274-1285
- Krapp, A., Hofmann, B., Schäfer, C., Stitt, M., Regulation of the expression of rbcS and other photosynthetic genes by carbohydrates: A mechanism for the "sink-regulation" of photosynthesis? (1993) Plant J., 3, pp. 817-828
- Krapp, A., Stitt, M., An evaluation of direct and indirect mechanisms for the sink-regulation of photosynthesis in spinach: Changes in gas exchange, carbohydrates, metabolites, enzyme activities and steady-state transcript levels after cold-girdling source leaves (1995) Planta, 195, pp. 13-23
- Krishnamurthy, K., Heppler, M., Mitra, R., Blancaflor, E., Payton, M., Nelson, R.S., Verchot-Lubicz, J., The potato virus X TGBp3 protein associates with the ER network for virus cell-to-cell movement (2003) Virology, 309, pp. 135-151
- Krishnamurthy, K., Mitra, R., Payton, M.E., Verchot-Lubicz, J., Cell-to-cell movement of the PVX 12K, 8K, or coat proteins may depend on the host, leaf developmental stage, and the PVX 25K protein (2002) Virology, 300, pp. 269-281
- Lough, T.J., Netzler, N.E., Emerson, S.J., Sutherland, P., Carr, F., Beck, D.L., Lucas, W.J., Forster, R.L., Cell-to-cell movement of potexviruses: Evidence for a ribonucleicprotein complex involving the coat protein and first triple gene block protein (2000) Mol. Plant-Microbe Interact., 13, pp. 962-974
- Lough, T.J., Shash, K., Xoconostle-Cázares, B., Hofstra, K.R., Beck, D.L., Balmori, E., Molecular dissection of the mechanism by which potexvirus triple gene blocks mediate cell-to-cell transport of infectious RNA (1998) Mol. Plant Microbe Interact., 11, pp. 801-814
- Lucas, W.J., Olesinski, A., Hull, R.S., Haudenshield, J.S., Deom, C.M., Beachy, R.N., Influence of the tobacco mosaic virus 30 kDa movement protein on carbon metabolism and photosynthesis partitioning in transgenic tobacco plants (1993) Planta, 190, pp. 88-96
- Mitra, R., Krishnamurthy, K., Blancaflor, E., Payton, M., Nelson, R.S., Verchot-Lubicz, J., The potato virus X TGBp2 protein association with the endoplasmic reticulum plays a role in but is not sufficient for viral cell-to-cell movement (2003) Virology, 312, pp. 35-48
- Mittler, R., Shulaev, V., Lam, E., Coordinated activation of programmed cell death and defense mechanisms in transgenic tobacco plants expressing a bacterial proton pump (1995) Plant Cell, 7, pp. 29-42
- Morozov, S.Y., Solovyev, A.G., Triple gene block: Modular design of a multifunctional machine for plant virus movement (2003) J. Gen. Virol., 84, pp. 1351-1366
- Olesinski, A.A., Lucas, W.J., Galun, E., Wolf, S., Pleiotropic effects of tobacco mosaic virus movement protein on carbon metabolism in transgenic tobacco plants (1995) Planta, 197, pp. 118-126
- Orman, B.E., Celnik, R.M., Mandel, A.M., Torres, H.N., Mentaberry, A.N., Complete DNA sequence of a South American isolate of potato virus X (1990) Virus Res., 16, pp. 293-305
- Quirino, F.B., Noh, Y.-S., Himelblau, E., Amasino, R., Molecular aspects of leaf senescence (2000) Trends Plant Sci., 5, pp. 278-282
- Rouleau, M., Smith, R.J., Bancroft, J.B., MacKie, G.A., Purification, properties, and subcellular localization of foxtail mosaic potexvirus 26-kDa protein (1994) Virology, 204, pp. 254-265
- Rouleau, M., Smith, R.J., Bancroft, J.B., MacKie, G.A., Subcellular immunolocalization of the coat protein of two potexviruses in infected Chenopodium quinoa (1995) Virology, 214, pp. 314-318
- Solovyev, A.G., Stroganova, T.A., Zamyatnin, A.A., Fedorkin, O.N., Schiemann, J., Morozov, S.Y., Subcellular sorting of small membrane-associated triple gene block proteins: TGBp3-assisted targeting of TGBp2 (2000) Virology, 269, pp. 113-127
- Sonnewald, V., Brauer, M., Von Schaewen, A., Stitt, M., Willmitzer, L., Transgenic tobacco plants expressing yeast-derived invertase in either the cytosol, vacuole or apoplast: A powerful tool for studying sucrose metabolism and sink/source interactions (1991) Plant J., 1, pp. 95-106
- Takahashi, M., Shimamoto, K., Ehara, Y., Cauliflower mosaic virus gene VI causes growth suppression, development of necrotic spots and expression of defense-related genes in transgenic tobacco plants (1989) Mol. Gen. Genet., 216, pp. 188-194
- Tevini, M., Steinmuller, D., Composition and function of plastoglobuli. I. Lipid composition of leaves and plastoglobuli during beech senescence (1985) Planta, 163, pp. 91-96
- Voinnet, O., Lederer, C., Baulcombe, D.C., A viral movement protein prevents spread of the gene silencing signal in Nicotiana benthamiana (2000) Cell, 103, pp. 157-162
- Woolhouse, H.W., Regulation of senescence in the chloroplast (1987) Plant Senescence: Its Biochemistry and Physiology, pp. 132-146. , W.W. Thomson E.A. Nothnagel R.C. Huffaker American Society of Plant Physiologists Rockville
- Yang, Y., Ding, B., Baulcombe, D.C., Verchot, J., Cell-to-cell movement of the 25K protein of potato virus X is regulated by three other viral proteins (2000) Mol. Plant-Microbe Interact, 13, pp. 599-605
Citas:
---------- APA ----------
Kobayashi, K., Sarrobert, C., Ares, X., Rivero, M.M., Maldonado, S., Robaglia, C. & Mentaberry, A.
(2004)
. Over-expression of potato virus X TGBp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations. Plant Physiology and Biochemistry, 42(9), 731-738.
http://dx.doi.org/10.1016/j.plaphy.2004.07.010---------- CHICAGO ----------
Kobayashi, K., Sarrobert, C., Ares, X., Rivero, M.M., Maldonado, S., Robaglia, C., et al.
"Over-expression of potato virus X TGBp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations"
. Plant Physiology and Biochemistry 42, no. 9
(2004) : 731-738.
http://dx.doi.org/10.1016/j.plaphy.2004.07.010---------- MLA ----------
Kobayashi, K., Sarrobert, C., Ares, X., Rivero, M.M., Maldonado, S., Robaglia, C., et al.
"Over-expression of potato virus X TGBp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations"
. Plant Physiology and Biochemistry, vol. 42, no. 9, 2004, pp. 731-738.
http://dx.doi.org/10.1016/j.plaphy.2004.07.010---------- VANCOUVER ----------
Kobayashi, K., Sarrobert, C., Ares, X., Rivero, M.M., Maldonado, S., Robaglia, C., et al. Over-expression of potato virus X TGBp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations. Plant Physiol. Biochem. 2004;42(9):731-738.
http://dx.doi.org/10.1016/j.plaphy.2004.07.010