Artículo
Abstract:
UDP-Glc:protein transglucosylase (UPTG) [EC 2.4.1.112], the protein regarded as pivotal to starch biosynthesis initiation, was purified from potato (Solanum tuberosum) tubers by modification of previous procedures. The steps used for purification were: solubilization of the particulate potato tuber preparation, ion-exchange chromatographies on DEAE-Sepharose fast flow and Mono Q columns, and affinity chromatography on a UDP-hexanolamine-agarose column. The last step yielded an electrophoretically homogeneous, stable enzyme protein with 1063-fold purification and a reproducible yield of 27%. UPTG, which undergoes self-glucosylation in an UDP-Glc and Mn2+-dependent reaction, is homomeric, made up of 38-kDa subunits. Polyclonal antibodies raised in rabbits against the potato tuber 38-kDa polypeptide cross reacted strongly with the potato tuber UPTG and with the maize (Zea mays) endosperm enzyme as well, and neutralized (inhibited) potato tuber and maize endosperm UPTG activity (more than 90%). This is the first demonstration that highly specific antibodies raised against one single and even denatured-protein are capable of neutralizing UPTG activity. The availability of the purified protein and specific antibodies opens the way to molecular characterization of potato tuber UPTG and to studying its role in the initiation of starch synthesis.
Registro:
| Documento: | Artículo |
| Título: | Initiation of starch biosynthesis: Purification and characterization of UDP-glucose:protein transglucosylase from potato tubers |
| Autor: | Bocca, S.N.; Rothschild, A.; Tandecarz, J.S. |
| Filiación: | Inst. de Investigaciones Bioquimicas, Fac. de Ciencias Exactas/Naturales, Universidad de Buenos Aires, Patricias Argentinas 435, 1405 Buenos Aires, Argentina |
| Palabras clave: | Enzyme purification; potato tuber; Solanum tuberosum; starch synthesis initiation; UDP-glucose:protein transglucosylase |
| Año: | 1997 |
| Volumen: | 35 |
| Número: | 3 |
| Página de inicio: | 205 |
| Página de fin: | 212 |
| Título revista: | Plant Physiology and Biochemistry |
| Título revista abreviado: | PLANT PHYSIOL. BIOCHEM. |
| ISSN: | 09819428 |
| CODEN: | PPBIE |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09819428_v35_n3_p205_Bocca |
Citas:
---------- APA ----------
Bocca, S.N., Rothschild, A. & Tandecarz, J.S. (1997) . Initiation of starch biosynthesis: Purification and characterization of UDP-glucose:protein transglucosylase from potato tubers. Plant Physiology and Biochemistry, 35(3), 205-212.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09819428_v35_n3_p205_Bocca [ ]
---------- CHICAGO ----------
Bocca, S.N., Rothschild, A., Tandecarz, J.S. "Initiation of starch biosynthesis: Purification and characterization of UDP-glucose:protein transglucosylase from potato tubers" . Plant Physiology and Biochemistry 35, no. 3 (1997) : 205-212.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09819428_v35_n3_p205_Bocca [ ]
---------- MLA ----------
Bocca, S.N., Rothschild, A., Tandecarz, J.S. "Initiation of starch biosynthesis: Purification and characterization of UDP-glucose:protein transglucosylase from potato tubers" . Plant Physiology and Biochemistry, vol. 35, no. 3, 1997, pp. 205-212.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09819428_v35_n3_p205_Bocca [ ]
---------- VANCOUVER ----------
Bocca, S.N., Rothschild, A., Tandecarz, J.S. Initiation of starch biosynthesis: Purification and characterization of UDP-glucose:protein transglucosylase from potato tubers. PLANT PHYSIOL. BIOCHEM. 1997;35(3):205-212.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09819428_v35_n3_p205_Bocca [ ]
