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Abstract:

Blue light (BL) suppression accelerates the senescence rate of wheat (Triticum aestivum L.) leaves exposed to shading. In order to study whether this effect involves the alteration of different cytokinin (CK) metabolites, CK-degradation, as well as the expression profile of genes responsible of CK-perception, -inactivation, -reactivation and/or -turnover, leaf segments of 30 day-old plants were placed in boxes containing bi-distilled water and covered with blue (B) or green (G) light filters, which supplied a similar irradiance but differed in the percentage of BL transmitted (G << B). A neutral (N) filter was used as control. When appropriate, different CK metabolites or an inhibitor of CK-degradation were added in order to alter the endogenous CK levels. A rapid decrement of trans-zeatin (tZ) and cis-zeatin (cZ) content was observed after leaf excision, which progressed at a higher rate in treatment G than in the control and B treatments. Senescence progression correlated with an accumulation of glycosylated forms (particularly cZ-derivatives), and an increment of CK-degradation, both of which were slowed in the presence of BL. On the contrary, CK-reactivation (analyzed through TaGLU1-3 expression) was delayed in the absence of BL. When different CK were exogenously supplied, tZ was the only natural free base capable to emulate the senescence-retarding effect of BL. Even though the signaling components involved in the regulation of senescence rate and CK-homeostasis by BL remain elusive, our data suggest that changes in the expression profile and/or functioning of the transcription factor HY5 might play an important role. © 2018 Elsevier Masson SAS

Registro:

Documento: Artículo
Título:Blue light suppression alters cytokinin homeostasis in wheat leaves senescing under shading stress
Autor:Marchetti, C.F.; Škrabišová, M.; Galuszka, P.; Novák, O.; Causin, H.F.
Filiación:Department of Molecular Biology, Centre of the Region of Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc, 783 71, Czech Republic
Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University & Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Šlechtitelů 27, Olomouc, 783 71, Czech Republic
Institute of Biodiversity, Experimental and Applied Biology (IBBEA), CONICET-UBA, Department of Biodiversity and Experimental Biology (DBBE), University of Buenos Aires, Faculty of Exact and Natural Sciences, Ciudad Universitaria, Pab. II, C.A.B.A, C1428EGA, Argentina
Palabras clave:Blue light; Cytokinin metabolism; Gene expression; Leaf senescence; Shading stress; Triticum aestivum; chlorophyll; cytokinin; cytokinin oxidase; oxidoreductase; plant protein; transcriptome; gene expression regulation; genetics; homeostasis; light; metabolism; phylogeny; plant gene; plant leaf; radiation response; real time polymerase chain reaction; wheat; Chlorophyll; Cytokinins; Gene Expression Regulation, Plant; Genes, Plant; Homeostasis; Light; Oxidoreductases; Phylogeny; Plant Leaves; Plant Proteins; Real-Time Polymerase Chain Reaction; Transcriptome; Triticum
Año:2018
Volumen:130
Página de inicio:647
Página de fin:657
DOI: http://dx.doi.org/10.1016/j.plaphy.2018.08.005
Título revista:Plant Physiology and Biochemistry
Título revista abreviado:Plant Physiol. Biochem.
ISSN:09819428
CODEN:PPBIE
CAS:chlorophyll, 1406-65-1, 15611-43-5; oxidoreductase, 9035-73-8, 9035-82-9, 9037-80-3, 9055-15-6; Chlorophyll; cytokinin oxidase; Cytokinins; Oxidoreductases; Plant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09819428_v130_n_p647_Marchetti

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Citas:

---------- APA ----------
Marchetti, C.F., Škrabišová, M., Galuszka, P., Novák, O. & Causin, H.F. (2018) . Blue light suppression alters cytokinin homeostasis in wheat leaves senescing under shading stress. Plant Physiology and Biochemistry, 130, 647-657.
http://dx.doi.org/10.1016/j.plaphy.2018.08.005
---------- CHICAGO ----------
Marchetti, C.F., Škrabišová, M., Galuszka, P., Novák, O., Causin, H.F. "Blue light suppression alters cytokinin homeostasis in wheat leaves senescing under shading stress" . Plant Physiology and Biochemistry 130 (2018) : 647-657.
http://dx.doi.org/10.1016/j.plaphy.2018.08.005
---------- MLA ----------
Marchetti, C.F., Škrabišová, M., Galuszka, P., Novák, O., Causin, H.F. "Blue light suppression alters cytokinin homeostasis in wheat leaves senescing under shading stress" . Plant Physiology and Biochemistry, vol. 130, 2018, pp. 647-657.
http://dx.doi.org/10.1016/j.plaphy.2018.08.005
---------- VANCOUVER ----------
Marchetti, C.F., Škrabišová, M., Galuszka, P., Novák, O., Causin, H.F. Blue light suppression alters cytokinin homeostasis in wheat leaves senescing under shading stress. Plant Physiol. Biochem. 2018;130:647-657.
http://dx.doi.org/10.1016/j.plaphy.2018.08.005