Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Collagen-induced arthritis in DBA/1 mice is a model of rheumatoid arthritis with marked synovitis and erosions. The disease can be adoptively transferred to SCID mice with arthritogenic splenocytes from DBA/1 mice injected with bovine collagen type II. However, infection of arthritogenic splenocytes with a retrovirus expressing TGF β1 inhibits development of arthritis in SCID mice. When DBA/1 mice, at onset of arthritis have additional arthritgenic splenocytes transferred, exacerbation occurs, reflected in a rapid increase in the number of arthritic joints, increased paw swelling and higher levels of anti-collagen antibody. By infecting arthritogenic splenocytes ex vivo with TGF β1 retrovirus, this exacerbation was inhibited. TGF β1 was effective in lowering inflammation of joints with already established arthritis and inhibiting the spreading of the disease to other joints. Transient reduction on anti-collagen antibody levels could also be obtained using purified T cells infected woth TGF β1 retrovirus. In addition, expression of TGF β1 in lymphocytes reduced the levels of gelatinase (MMP2) activity in inflamed joints.

Registro:

Documento: Artículo
Título:Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model
Autor:Chernajovsky, Y.; Adams, G.; Triantaphyllopoulos, K.; Ledda, M.F.; Podhajcer, O.L.
Filiación:Kennedy Institute of Rheumatology, Molecular Biology Laboratory, London, United Kingdom
Fundación Campomar, FCEYN Universidad de Buenos Aires, Argentina
Molecular Biology Laboratory, Kennedy Institute of Rheumatology, 6 Bute Gardens, Hammersmith, London W6 7DW, United Kingdom
Palabras clave:Cytokine receptors; Cytokines; Rheumatoid arthritis; T lymphocytes; TGF β1 retrovirus; collagen; collagen antibody; gelatinase; transforming growth factor beta1; animal cell; animal experiment; animal model; animal tissue; antiinflammatory activity; article; enzyme activity; gene transfer; lymphoid cell; male; mouse; nonhuman; paw edema; priority journal; protein expression; Retrovirus; rheumatoid arthritis; spleen cell; synovitis; T lymphocyte; Animalia; Bovinae; unidentified retrovirus
Año:1997
Volumen:4
Número:6
Página de inicio:553
Página de fin:559
DOI: http://dx.doi.org/10.1038/sj.gt.3300436
Título revista:Gene Therapy
Título revista abreviado:GENE THER.
ISSN:09697128
CODEN:GETHE
CAS:collagen, 9007-34-5; gelatinase, 9040-48-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09697128_v4_n6_p553_Chernajovsky

Referencias:

  • Feldmann, M., Brennan, F.M., Maini, R.N., Role of cytokines in rheumatoid arthritis (1996) Ann Rev Immunol, 14, pp. 397-440
  • Barcellos-Hoff, M.H., TGF β activation in irradiated murine mammary gland (1994) J Clin Invest, 93, pp. 892-899
  • Roberts, A.B., Sporn, M.B., The transforming growth factor-betas (1990) Peptide Growth Factors and Their Receptors, pp. 419-472. , Sporn M, Roberts A (eds). Springer Verlag: New York
  • Brennan, F.M., Detection of TGF-β in rheumatoid arthritis synovial tissue: Lack of effect on spontaneous cytokine production in joint cell cultures (1990) Clin Exp Immunol, 81, pp. 278-285
  • Kuruvilla, A.P., Protective effect of TGF β1 on experimental autoimmune diseases in mice (1991) Proc Natl Acad Sci USA, 88, pp. 2918-2921
  • Brandes, M.E., TGF β1 suppresses acute and chronic arthritis in experimental animals (1991) J Clin Invest, 87, pp. 1108-1113
  • Allen, J.B., Rapid onset synovial inflammation and hyperplasia induced by TGF β (1990) J Exp Med, 171, pp. 231-247
  • Shull, M.M., Targeted disruption of the mouse TGF-β1 gene results in multifocal inflammatory disease (1992) Nature, 359, pp. 693-699
  • Montani, M., Prevention of adoptively transferred diabetes in NOD mice with IL-10-transduced islet-specific Th1 lymphocytes (1996) J Clin Invest, 98, pp. 1851-1859
  • Williams, R.O., Successful transfer of collagen-induced arthritis to SCID mice (1992) Clin Exp Immunol, 88, pp. 455-460
  • Chernajovsky, Y., Inhibition of transfer of collagen-induced arthritis into SCID mice by ex vivo infection of spleen cells with retroviruses expressing soluble human TNF receptor (1995) Gene Therapy, 2, pp. 731-735
  • Aoyama, A., Chen, W.-T., A 170 kDa membrane-bound protease is associated with the expression of invasiveness by human malignant melanoma (1990) Proc Natl Acad Sci USA, 87, pp. 8296-8300
  • Fontana, A., Modulation of the immune response by TGF β (1992) Int Arch Allergy Immunol, 99, pp. 1-7
  • Günther, M., TGFβ1 regulates TIMP-1 expression in differentiated articular chondrocytes (1994) Arth Rheum, 37, pp. 395-405
  • Morales, T.I., Roberts, A.B., TGF β regulates the metabolism of proteoglycans in bovine cartilage organ cultures (1988) J Biol Chem, 263, pp. 12828-12831
  • Raz, E., Systemic immunological effects of cytokine genes injected into skeletal-muscle (1993) Proc Natl Acad Sci USA, 90, pp. 4523-4527
  • Raz, E., Modulation of disease activity in murine lupus erythematosus by cytokine gene delivery (1995) Lupus, 4, pp. 286-292
  • Giladi, E., TGFβ gene therapy ameliorates experimental colitis in rats (1995) Eur J Gastroent Hepatol, 7, pp. 341-347
  • Durie, F.H., Prevention of collagen induced arthritis with an antibody to gp39, the ligand to CD40 (1993) Science, 261, pp. 1328-1330
  • Williams, R.O., Feldmann, M., Maini, R.N., Anti-TNF ameliorates joint disease in murine collagen-induced arthritis (1992) Proc Natl Acad Sci USA, 89, pp. 9784-9788
  • Watson, W.C., Townes, A.S., Genetic susceptibility to murine collagen II autoimmune arthritis (1985) J Exp Med, 162, pp. 1878-1891
  • Mori, I., Expression of a transgenic T cell receptor β chain enhances collagen-induced arthritis (1992) J Exp Med, 176, pp. 381-388
  • Wang, Y., Anti-C5 monoclonal antibody therapy prevents collagen-induced arthritis and ameliorates established disease (1995) Proc Natl Acad Sci USA, 92, pp. 8955-8959
  • Arsura, M., Wu, M., Soneshein, G., TGF β1 inhibits NF-κB/Rel activity inducing apoptosis of B cells: Transcriptional activation of IκBα (1996) Immunity, 5, pp. 31-40
  • Khoury, S.J., Hancock, W.W., Weiner, H.L., Oral tolerance to MBP and natural recovery from EAE are associated with down-regulation of inflammatory cytokines and differential upregulation of TGF β, IL-4 and prostaglandin E expression in the brain (1992) J Exp Med, 176, pp. 1355-1364
  • Garcia, B., Differential effects of TGFβ1 on IgA vs IgG2b production by LPS-stimulated lymph node B cells: A comparative study with spleen B cells (1996) Eur J Immunol, 26, pp. 2364-2370
  • Maier, R., Ganu, V., Lotz, M., IL-11, an inducible cytokine in human articular chondrocytes and synoviocytes, stimulates the production of the tissue inhibitor of metalloproteinases (1993) J Biol Chem, 268, pp. 21527-21532
  • Kramer, R., Gene transfer through the blood-nerve barrier: NGF-engineered neuritogenic T lymphocytes attenuate experimental autoimmune neuritis (1995) Nature Med, 1, pp. 1162-1166
  • Holmdahl, R., Homologous type II collagen induces chronic and progressive arthritis in mice (1986) Arth Rheum, 29, pp. 106-113
  • Morgenstern, J.P., Land, H., Advanced mammalian gene transfer: High titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line (1990) Nucleic Acids Res, 18, pp. 3587-3596
  • Markowitz, D., Goff, S., Bank, A., Construction and use of a safe and efficient amphotropic packaging cell line (1988) Virology, 167, pp. 400-406
  • Chernajovsky, Y., Kirby-Sanders, H., A cis-acting sequence, located at -450 in the promoter of the human interferon-inducible gene 6-16, binds constitutively to a nuclear protein and decreases the expression of a reporter interferon-inducible promoter (1990) Lymph Res, 9, pp. 199-212
  • Healey, D.G., Parker, D., Turk, J.L., Distribution of guinea pig lymphocyte cell surface antigen of defined function, as detected by the mouse monoclonal antibody MSgp1 (1987) Int Arch Allergy Appl Immun, 82, pp. 113-119
  • Samelson, L.E., T cell antigen receptor phosphorylation induced by an anti-receptor antibody (1987) J Immunol, 139, pp. 2708-2714
  • Woessner Jr., J.F., Quantification of matrix metalloproteinases in tissue samples (1995) Methods in Enzymology, 248, pp. 510-528. , Academic Press: Mew York
  • Kairiyama, C., Biologic, immunocytochemical, and cytogenetic characterization of two new human-melanoma cell-lines 1I-MEL-LES and 1IB-MEL-IAN (1995) Pigment Cell Res, 8, pp. 121-131

Citas:

---------- APA ----------
Chernajovsky, Y., Adams, G., Triantaphyllopoulos, K., Ledda, M.F. & Podhajcer, O.L. (1997) . Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model. Gene Therapy, 4(6), 553-559.
http://dx.doi.org/10.1038/sj.gt.3300436
---------- CHICAGO ----------
Chernajovsky, Y., Adams, G., Triantaphyllopoulos, K., Ledda, M.F., Podhajcer, O.L. "Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model" . Gene Therapy 4, no. 6 (1997) : 553-559.
http://dx.doi.org/10.1038/sj.gt.3300436
---------- MLA ----------
Chernajovsky, Y., Adams, G., Triantaphyllopoulos, K., Ledda, M.F., Podhajcer, O.L. "Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model" . Gene Therapy, vol. 4, no. 6, 1997, pp. 553-559.
http://dx.doi.org/10.1038/sj.gt.3300436
---------- VANCOUVER ----------
Chernajovsky, Y., Adams, G., Triantaphyllopoulos, K., Ledda, M.F., Podhajcer, O.L. Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model. GENE THER. 1997;4(6):553-559.
http://dx.doi.org/10.1038/sj.gt.3300436