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Abstract:

Different types of voltage-dependent calcium channels (VDCCs) have been recognized based on their molecular structure as well as their pharmacological and biophysical properties. One of these, the P/Q type, is the main channel involved in nerve evoked neurotransmitter release at neuromuscular junctions (NMJs) and many central nervous system synapses. However, under particular experimental or biological conditions, other channels can be involved. L-type VDCC presence at the NMJ has been demonstrated by the contribution to the perineural calcium currents (ICa) at adult mice Bapta-loaded NMJs. This is probably a result of a reduction in Ca2+ inactivation. The L-type current was not coupled to neurotransmitter release, but became coupled, as demonstrated by the release of acetylcholine, after the inhibition of serine/threonine protein phosphatases with okadaic acid (OA). Thus, under these conditions, L-type channels were unmasked at Bapta- but not at Egta-loaded NMJs. This suggests that the speed, not the capacity, of the calcium chelator was decisive in preventing Ca2+-inactivation and facilitating the contribution to neurotransmitter release. At neonatal rat NMJs, N-type VDCCs were involved early during development whereas P/Q-type VDCCs play a main role at all stages of development. Furthermore, P/Q-type VDCCs were more efficiently coupled to neurotransmitter release than N-type VDCCs. This difference could be accounted for by a differential location of these channels at the release site. Neuromuscular transmission in P/Q-type calcium channel knock out ataxic mice jointly depends on both N-type and R-type channels and shows several altered properties including low quantal content. Thus, calcium channels may be recruited to mediate neurotransmitter release with a functional hierarchy where the P/Q channel seems to be the channel most suited to mediate exocytosis at NMJs.

Registro:

Documento: Artículo
Título:Calcium channels involved in neurotransmitter release at adult, neonatal and P/Q-type deficient neuromuscular junctions (review)
Autor:Urbano, F.J.; Rosato-Siri, M.D.; Uchitel, O.D.
Filiación:Laboratorio de Fisiologia Molecular, Departamento de Ciencias Biologicas, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
Biophysics Sector S.I.S.S.A./I.S.A.S, Via Beirut 2-4, 34014 Trieste, Italy
Palabras clave:Bapta; Calcium channels; Development; Egta; Neuromuscular junction; calcium channel N type; calcium channel P type; calcium channel Q type; calcium channel R type; calcium ion; egtazic acid; ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid; neurotransmitter; animal cell; controlled study; knockout mouse; mouse; neuromuscular synapse; neuromuscular transmission; neurotransmitter release; newborn; nonhuman; priority journal; protein expression; rat; review; Animals; Calcium Channels; Calcium Channels, L-Type; Mice; Neuromuscular Junction; Neuromuscular Junction Diseases; Neurotransmitter Agents; Rats; Animalia
Año:2002
Volumen:19
Número:4
Página de inicio:293
Página de fin:300
DOI: http://dx.doi.org/10.1080/0968768021000035087
Título revista:Molecular Membrane Biology
Título revista abreviado:Mol. Membr. Biol.
ISSN:09687688
CODEN:MMEBE
CAS:calcium ion, 14127-61-8; egtazic acid, 67-42-5; ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid, 73630-08-7; Calcium Channels; Calcium Channels, L-Type; Neurotransmitter Agents
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09687688_v19_n4_p293_Urbano

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Citas:

---------- APA ----------
Urbano, F.J., Rosato-Siri, M.D. & Uchitel, O.D. (2002) . Calcium channels involved in neurotransmitter release at adult, neonatal and P/Q-type deficient neuromuscular junctions (review). Molecular Membrane Biology, 19(4), 293-300.
http://dx.doi.org/10.1080/0968768021000035087
---------- CHICAGO ----------
Urbano, F.J., Rosato-Siri, M.D., Uchitel, O.D. "Calcium channels involved in neurotransmitter release at adult, neonatal and P/Q-type deficient neuromuscular junctions (review)" . Molecular Membrane Biology 19, no. 4 (2002) : 293-300.
http://dx.doi.org/10.1080/0968768021000035087
---------- MLA ----------
Urbano, F.J., Rosato-Siri, M.D., Uchitel, O.D. "Calcium channels involved in neurotransmitter release at adult, neonatal and P/Q-type deficient neuromuscular junctions (review)" . Molecular Membrane Biology, vol. 19, no. 4, 2002, pp. 293-300.
http://dx.doi.org/10.1080/0968768021000035087
---------- VANCOUVER ----------
Urbano, F.J., Rosato-Siri, M.D., Uchitel, O.D. Calcium channels involved in neurotransmitter release at adult, neonatal and P/Q-type deficient neuromuscular junctions (review). Mol. Membr. Biol. 2002;19(4):293-300.
http://dx.doi.org/10.1080/0968768021000035087