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Abstract:

The present report shows a partial biochemical characterization and life cycle expression of N-β-alanyldopamine hydrolase (Tan protein) in Ceratitis capitata and Drosophila melanogaster. This enzyme catalyzes the hydrolysis of N-β-alanyldopamine (NBAD), the main tanning precursor of insect brown cuticles. It also plays an important role in the metabolism of brain neurotransmitters, recycling dopamine and histamine. In contrast to NBAD-synthase, Tan is expressed constitutively in epidermis and does not respond directly to microbial challenge. Immunodetection experiments showed the novel localization of NBAD-hydrolase in the embryo central neural system and in different regions of the adult brain, in addition to optic lobes. We sequenced and characterized Drosophila mutants tan1 and tan3. The latter appears to be a mutant with normal expression in neural tissue but weak one in epidermis. © 2011 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants
Autor:Pérez, M.M.; Sabio, G.; Badaracco, A.; Quesada-Allué, L.A.
Filiación:Department of Biological Chemistry, FCEyN, University of Buenos Aires, IIBBA-CONICET and Fundación Instituto Leloir, Patricias Argentinas 435, Buenos Aires 1405, Argentina
Palabras clave:β-alanylderivatives; N-β-alanyldopamine; NBAD-hydrolase; NBAD-synthase; DNA binding protein; Drosophila protein; insect protein; nonhistone protein; tan protein, Drosophila; animal; article; brain; dermis; Drosophila melanogaster; enzymology; gene expression regulation; genetics; growth, development and aging; insect; larva; Mediterranean fruit fly; metabolism; molecular genetics; nucleotide sequence; phenotype; Animals; Base Sequence; Brain; Ceratitis capitata; Chromosomal Proteins, Non-Histone; Dermis; DNA-Binding Proteins; Drosophila melanogaster; Drosophila Proteins; Gene Expression Regulation, Developmental; Insect Proteins; Larva; Molecular Sequence Data; Phenotype; Pupa; Ceratitis capitata; Drosophila melanogaster; Hexapoda
Año:2011
Volumen:41
Número:9
Página de inicio:653
Página de fin:659
DOI: http://dx.doi.org/10.1016/j.ibmb.2011.04.007
Título revista:Insect Biochemistry and Molecular Biology
Título revista abreviado:Insect Biochem. Mol. Biol.
ISSN:09651748
CODEN:IBMBE
CAS:Chromosomal Proteins, Non-Histone; DNA-Binding Proteins; Drosophila Proteins; Insect Proteins; tan protein, Drosophila
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09651748_v41_n9_p653_Perez

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Citas:

---------- APA ----------
Pérez, M.M., Sabio, G., Badaracco, A. & Quesada-Allué, L.A. (2011) . Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants. Insect Biochemistry and Molecular Biology, 41(9), 653-659.
http://dx.doi.org/10.1016/j.ibmb.2011.04.007
---------- CHICAGO ----------
Pérez, M.M., Sabio, G., Badaracco, A., Quesada-Allué, L.A. "Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants" . Insect Biochemistry and Molecular Biology 41, no. 9 (2011) : 653-659.
http://dx.doi.org/10.1016/j.ibmb.2011.04.007
---------- MLA ----------
Pérez, M.M., Sabio, G., Badaracco, A., Quesada-Allué, L.A. "Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants" . Insect Biochemistry and Molecular Biology, vol. 41, no. 9, 2011, pp. 653-659.
http://dx.doi.org/10.1016/j.ibmb.2011.04.007
---------- VANCOUVER ----------
Pérez, M.M., Sabio, G., Badaracco, A., Quesada-Allué, L.A. Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants. Insect Biochem. Mol. Biol. 2011;41(9):653-659.
http://dx.doi.org/10.1016/j.ibmb.2011.04.007