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Abstract:

Optimization of laccase production in white-rot fungi has been extensively studied. Metallic and aromatic compounds have been found to enhance enzyme production, but the development of bioremediation as an application field of this enzyme requires clean technologies. In this work, co-cultivation of Ganoderma lucidum and Trametes versicolor was performed, showing remarkable enhancement of laccase activity. Dual cultures were assayed for malachite green degradation (MG) in solid state fermentation (SSF) using a sawdust-based medium. The time for achieving complete decolorization of MG in co-cultivation was markedly shorter than that observed in monocultures. Dual-species treatment did not differ in wood dry weight loss and lignin, cellulose and hemicellulose degradation, compared to monocultures; but the selectivity index (lignin loss/cellulose loss) of dual cultures was markedly higher than those attained by monocultures. Moreover, a modified isoenzymatic laccase pattern was observed, showing one isoenzyme that was absent in monocultures. Dual cultures were able to decolorize and detoxify the dye more efficiently than the monocultures. The noticeable increase in laccase activity along with the more efficient decolorization and detoxification of MG by co-cultures of G.lucidum and T.versicolor in SSF makes this system a viable strategy for large scale application of white-rot cultures in bioremediation. © 2015 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation
Autor:Kuhar, F.; Castiglia, V.; Levin, L.
Filiación:Laboratorio de Micología Experimental, Departamento de Biodiversidad y Biologia Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Ciudad de Buenos Aires, Argentina
Palabras clave:Co-cultivation; Laccase; Malachite green; White-rot fungi; Bioremediation; Biotechnology; Carbonate minerals; Cellulose; Detoxification; Fermentation; Fungi; Lignin; Wood; Cellulose and hemicellulose; Co-cultivation; Laccases; Large-scale applications; Malachite green; Solid-state fermentation; Trametes versicolor; White rot fungi; Enzymes; bioremediation; color; enzyme activity; fermentation; fungal disease; fungus; malachite; monoculture; optimization; Fermentation; Laccase; Production; Fungi; Ganoderma lucidum; Trametes versicolor
Año:2015
Volumen:104
Página de inicio:238
Página de fin:243
DOI: http://dx.doi.org/10.1016/j.ibiod.2015.06.017
Título revista:International Biodeterioration and Biodegradation
Título revista abreviado:Int. Biodeterior. Biodegrad.
ISSN:09648305
CODEN:IBBIE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09648305_v104_n_p238_Kuhar

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Citas:

---------- APA ----------
Kuhar, F., Castiglia, V. & Levin, L. (2015) . Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation. International Biodeterioration and Biodegradation, 104, 238-243.
http://dx.doi.org/10.1016/j.ibiod.2015.06.017
---------- CHICAGO ----------
Kuhar, F., Castiglia, V., Levin, L. "Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation" . International Biodeterioration and Biodegradation 104 (2015) : 238-243.
http://dx.doi.org/10.1016/j.ibiod.2015.06.017
---------- MLA ----------
Kuhar, F., Castiglia, V., Levin, L. "Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation" . International Biodeterioration and Biodegradation, vol. 104, 2015, pp. 238-243.
http://dx.doi.org/10.1016/j.ibiod.2015.06.017
---------- VANCOUVER ----------
Kuhar, F., Castiglia, V., Levin, L. Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation. Int. Biodeterior. Biodegrad. 2015;104:238-243.
http://dx.doi.org/10.1016/j.ibiod.2015.06.017