Artículo

La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Inactivation of three Saccharomyces cerevisiae strains by PEF treatment, at different growth phases and initial inoculum sizes was investigated. The conditions of the PEF treatment were: electric field level of 8-11 kV/cm, 3 pulses, 50 μF capacitance or 12.5 kV/cm, 40 pulses, 1 μF capacitance. The inactivation of yeasts ranged from 2 to 5.8 log cycles when the inoculum size increased from 5×105 to 1×1011 CFU/ml, respectively. In all the experiments, the reduction of yeasts population was linearly enhanced by increasing the inoculum size, independently of the growth phase. Different experiments were designed to explain the observed behavior. By increasing yeasts concentration the probability of formation of pearl chains would increase. As a consequence of the large volume of these "equivalent cells" the PEF treatment efficiency would increase. © 2004 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields
Autor:Molinari, P.; Pilosof, A.M.R.; Jagus, R.J.
Filiación:Departamento de Industrias, Fac. de Ciencias Exactas Y Naturales, Ciudad Universitaria (1428), Buenos Aires, Argentina
Depto. de Ing. Industrial, Facultad de Ingeniería, Ciudad Universitaria (1428), Buenos Aires, Argentina
Consejo, Nac. Invest., Cie. Y T.
Palabras clave:Concentration; Pulsed electric field; Yeast; Capacitance; Electric fields; Fruit juices; Yeast; growth phases; Inoculum size; Food products; Saccharomyces; Saccharomyces cerevisiae
Año:2004
Volumen:37
Número:8
Página de inicio:793
Página de fin:798
DOI: http://dx.doi.org/10.1016/j.foodres.2004.03.010
Título revista:Food Research International
Título revista abreviado:Food Res. Int.
ISSN:09639969
CODEN:FORIE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09639969_v37_n8_p793_Molinari

Referencias:

  • Alvarez, I., Raso, J., Palop, A., Sala, F.J., Influence of different factors on the inactivation of Salmonella senftemberg by pulsed electric fields (2000) International Journal of Food Microbiology, 55, pp. 143-146
  • Barbosa-Cànovas, G.V., Góngora-Nieto, M.M., Pothakamury, V.R., Swanson, B.G., (1999) Preservation of Food with Pulsed Electric Fields, , London: Academic Press
  • Buera, M.P., Chirife, J., Karel, M., A study of acid catalyzed sucrose hydrolysis in an amorphous polymeric matrix at reduced moisture content (1995) Food Research International, 28, pp. 359-365
  • Castro, A.J., Barbosa-Canovas, G.V., Swanson, B.G., Microbial inactivation of food by pulsed electric field (1993) Journal of Food Processing and Preservation, 17, pp. 47-73
  • Gáskova, D., Sigler, K., Janderová, B., Pásek, J., Effect of high-voltage electric pulses and yest cells: Factors influencing the killing efficiency (1996) Bioelectrochemistry and Bioenergetics, 39, pp. 195-202
  • Grahl, T., Markl, H., Killing of microorganisms by pulsed electric fields (1996) Applied Microbiology and Biotechnology, 45, pp. 148-157
  • Hohmann, S., Mager, W.H., Molecular biology intelligence unit. Yeast stress responses (1997) Landes Bioscience, , Austin, TX, USA
  • Jayaram, S., Castle, G.S.P., Margaritis, A., Kinetics of sterilization of Lactobacillus brevis by the application of high voltage pulses (1992) Biotechnology and Bioengineering, 40 (11), pp. 1412-1420
  • Jayaram, S., Castle, G.S.P., Margaritis, A., The effect of high field DC pulse and liquid medium conductivity on survivability of Lactobacillus brevis (1993) Applied and Microbiology Biotechnology, 40, pp. 117-122
  • Kyzlink, V., (1990) Principles of Food Preservation, , New York: Elseiver
  • McDonald, C.J., Lloyd, S.W., Vitale, M.A., Petersson, K., Innings, F., Effect of pulsed electric field on microorganisms in orange juice using electric field strengths of 30 and 50 kV/cm (2000) Journal of Food Science, 65 (6), pp. 984-989
  • Murdock, D.I., Troy, V.S., Folinazzo, J.S., Thermal resistance of lactic acid bacteria and yeast in orange juice and concentrate (1953) Food Research, 18, pp. 85-89
  • Raso, J., Calderon, M.L., Gongora, M., Barbosa-Cánovas, G.V., Swanson, B.G., Inactivation of Zygosaccharomyces bailii in fruit juices by heat, high hydrostatic pressure and pulse electric fields (1998) Journal of Food Science, 83 (6), pp. 1042-1043
  • Sadler, G.D., Parish, M.D., Wicker, I., Microbial enzymatic, and chemical changes during storage of fresh and processed orange juice (1992) Journal of Food Science, 57, pp. 1187-1197
  • Sale, A.J.H., Hamilton, W.A., Effect of high electric fields on microorganisms I. Killing of bacteria and yeast (1967) Biochimia et Biophysica Acta, 163, pp. 781-788
  • Sharma, S.K., Zhang, Q.H., Chism, G.W., Development of a protein fortified fruit beverage and its quality when processed with pulsed electric field treatment (1998) Journal of Food Quality, 21, pp. 459-473
  • Vega-Mercado, H., Pothakamury, U.R., Chang, F.J., Barbosa-Canovas, G.V., Swanson, B.G., Inactivation of Escherichia coli by combining pH, ionic strngth and pulsed electric fields hurdles (1996) Food Research International, 29, pp. 117-121
  • Wouters, P.C., Dutreux, N., Smelt, J.P.P.H., Llieveld, H.I.M., Effects of pulsed electric fields on inactivation kinetics of Listeria innocua (1999) Applied and Environmental Microbiology, 12 (65), pp. 5364-5371
  • Zhang, Q., Monsalve-Gonzalez, A., Qin, B.L., Barbosa-Canovas, G.V., Swanson, B.G., Inactivation of Saccharomyces cerevisiae by square wave and exponential decay electric field (1994) Journal of Food Process Engineering, 17, pp. 469-478
  • Zimmermann, U., Electric field-mediated fusion and related electrical phenomena (1992) Biochimica et Biophysica Acta, 694, pp. 227-277
  • Zimmermann, U., Electrical breakdown, electropermeabilization and electrofusion (1986) Reviews of Physiology Biochemisty and Pharmacology, 105, pp. 176-256
  • Zimmermann, U., The effect of high intensity electric field pulses on eucaryotic cell membrane: Fundamentals and applications (1996) Electromanipulation of Cells, pp. 1-106. , U. Zimmermann, & G.A. Neil. Boca Raton: CRC Press

Citas:

---------- APA ----------
Molinari, P., Pilosof, A.M.R. & Jagus, R.J. (2004) . Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields. Food Research International, 37(8), 793-798.
http://dx.doi.org/10.1016/j.foodres.2004.03.010
---------- CHICAGO ----------
Molinari, P., Pilosof, A.M.R., Jagus, R.J. "Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields" . Food Research International 37, no. 8 (2004) : 793-798.
http://dx.doi.org/10.1016/j.foodres.2004.03.010
---------- MLA ----------
Molinari, P., Pilosof, A.M.R., Jagus, R.J. "Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields" . Food Research International, vol. 37, no. 8, 2004, pp. 793-798.
http://dx.doi.org/10.1016/j.foodres.2004.03.010
---------- VANCOUVER ----------
Molinari, P., Pilosof, A.M.R., Jagus, R.J. Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields. Food Res. Int. 2004;37(8):793-798.
http://dx.doi.org/10.1016/j.foodres.2004.03.010