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Abstract:

The synthesis of modified nucleosides has received a great deal of attention due to their applications as antiviral and antitumoral agents. Among the different synthetic strategies, microbial transglycosylation has already shown to provide successful results. In the present work, we analyse the use of Citrobacter amalonaticus CECT 863 cells - free or immobilised - in the synthesis of some modified nucleosides. The main characteristics of the immobilised biocatalysts such as type and concentration of polymer, mechanical and storage stability and reuse were assessed. From these studies, polyacrylamide was selected as the best support based on its performance and potential industrial applications. In particular, it can be reused more than 50 times without significant loss of activity.

Registro:

Documento: Artículo
Título:Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis
Autor:Trelles, J.A.; Lewkowicz, E.S.; Sinisterra, J.V.; Iribarren, A.M.
Filiación:Universidad Nacional de Quilmes, R.S. Peña 180, (1876) Bernal, Buenos Aires, Argentina
Depto. de Quim. Organ. y Farmaceut., Facultad de Farmacia, Universidad Complutense de Madrid, E-28040 Madrid, Spain
INGEBI, CONICET, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
University of Quilmes, Argentina
University of Buenos Aires, Argentina
Universidad Complutense de Madrid, Spain
Pharmaceutical Chemistry, Universidad Complutense de Madrid, Spain
Palabras clave:Cell immobilisation; Citrobacter amalonaticus; Microbial transglycosylation; Modified nucleosides; Polyacrylamide; Concentration (process); Performance; Polymers; Synthesis (chemical); Antitumoral agents; Antiviral agents; Citrobacter amalonaticus; Polyacrylamide; Biocatalysts; nucleoside; polyacrylamide; catalysis; article; bacterial metabolism; catalysis; Citrobacter; citrobacter amalonaticus; glycosylation; immobilized cell; nonhuman; nucleotide metabolism; Bacteria (microorganisms); Citrobacter; Citrobacter amalonaticus
Año:2004
Volumen:6
Número:4
Página de inicio:376
Página de fin:384
DOI: http://dx.doi.org/10.1504/IJBT.2004.005520
Título revista:International Journal of Biotechnology
Título revista abreviado:Int. J. Biotechnol.
ISSN:09636048
CODEN:IJBNB
CAS:polyacrylamide, 9003-05-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09636048_v6_n4_p376_Trelles

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Citas:

---------- APA ----------
Trelles, J.A., Lewkowicz, E.S., Sinisterra, J.V. & Iribarren, A.M. (2004) . Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis. International Journal of Biotechnology, 6(4), 376-384.
http://dx.doi.org/10.1504/IJBT.2004.005520
---------- CHICAGO ----------
Trelles, J.A., Lewkowicz, E.S., Sinisterra, J.V., Iribarren, A.M. "Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis" . International Journal of Biotechnology 6, no. 4 (2004) : 376-384.
http://dx.doi.org/10.1504/IJBT.2004.005520
---------- MLA ----------
Trelles, J.A., Lewkowicz, E.S., Sinisterra, J.V., Iribarren, A.M. "Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis" . International Journal of Biotechnology, vol. 6, no. 4, 2004, pp. 376-384.
http://dx.doi.org/10.1504/IJBT.2004.005520
---------- VANCOUVER ----------
Trelles, J.A., Lewkowicz, E.S., Sinisterra, J.V., Iribarren, A.M. Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis. Int. J. Biotechnol. 2004;6(4):376-384.
http://dx.doi.org/10.1504/IJBT.2004.005520