Abstract:
The synthesis of modified nucleosides has received a great deal of attention due to their applications as antiviral and antitumoral agents. Among the different synthetic strategies, microbial transglycosylation has already shown to provide successful results. In the present work, we analyse the use of Citrobacter amalonaticus CECT 863 cells - free or immobilised - in the synthesis of some modified nucleosides. The main characteristics of the immobilised biocatalysts such as type and concentration of polymer, mechanical and storage stability and reuse were assessed. From these studies, polyacrylamide was selected as the best support based on its performance and potential industrial applications. In particular, it can be reused more than 50 times without significant loss of activity.
Registro:
Documento: |
Artículo
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Título: | Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis |
Autor: | Trelles, J.A.; Lewkowicz, E.S.; Sinisterra, J.V.; Iribarren, A.M. |
Filiación: | Universidad Nacional de Quilmes, R.S. Peña 180, (1876) Bernal, Buenos Aires, Argentina Depto. de Quim. Organ. y Farmaceut., Facultad de Farmacia, Universidad Complutense de Madrid, E-28040 Madrid, Spain INGEBI, CONICET, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina University of Quilmes, Argentina University of Buenos Aires, Argentina Universidad Complutense de Madrid, Spain Pharmaceutical Chemistry, Universidad Complutense de Madrid, Spain
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Palabras clave: | Cell immobilisation; Citrobacter amalonaticus; Microbial transglycosylation; Modified nucleosides; Polyacrylamide; Concentration (process); Performance; Polymers; Synthesis (chemical); Antitumoral agents; Antiviral agents; Citrobacter amalonaticus; Polyacrylamide; Biocatalysts; nucleoside; polyacrylamide; catalysis; article; bacterial metabolism; catalysis; Citrobacter; citrobacter amalonaticus; glycosylation; immobilized cell; nonhuman; nucleotide metabolism; Bacteria (microorganisms); Citrobacter; Citrobacter amalonaticus |
Año: | 2004
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Volumen: | 6
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Número: | 4
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Página de inicio: | 376
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Página de fin: | 384
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DOI: |
http://dx.doi.org/10.1504/IJBT.2004.005520 |
Título revista: | International Journal of Biotechnology
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Título revista abreviado: | Int. J. Biotechnol.
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ISSN: | 09636048
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CODEN: | IJBNB
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CAS: | polyacrylamide, 9003-05-8
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09636048_v6_n4_p376_Trelles |
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Citas:
---------- APA ----------
Trelles, J.A., Lewkowicz, E.S., Sinisterra, J.V. & Iribarren, A.M.
(2004)
. Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis. International Journal of Biotechnology, 6(4), 376-384.
http://dx.doi.org/10.1504/IJBT.2004.005520---------- CHICAGO ----------
Trelles, J.A., Lewkowicz, E.S., Sinisterra, J.V., Iribarren, A.M.
"Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis"
. International Journal of Biotechnology 6, no. 4
(2004) : 376-384.
http://dx.doi.org/10.1504/IJBT.2004.005520---------- MLA ----------
Trelles, J.A., Lewkowicz, E.S., Sinisterra, J.V., Iribarren, A.M.
"Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis"
. International Journal of Biotechnology, vol. 6, no. 4, 2004, pp. 376-384.
http://dx.doi.org/10.1504/IJBT.2004.005520---------- VANCOUVER ----------
Trelles, J.A., Lewkowicz, E.S., Sinisterra, J.V., Iribarren, A.M. Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis. Int. J. Biotechnol. 2004;6(4):376-384.
http://dx.doi.org/10.1504/IJBT.2004.005520