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Abstract:

Bordetella bronchiseptica produces respiratory disease primarily in mammals including humans. Although a considerably amount of research has been generated regarding lipopolysaccharide (LPS) role during infection and stimulating innate and adaptive immune response, mechanisms involved in LPS synthesis are still unknown. In this context we searched in B. bronchiseptica genome for putative glycosyltransferases. We found possible genes codifying for enzymes involved in sugar substitution of the LPS structure. We decided to analyse BB3394 to BB3400 genes, closed to a previously described LPS biosynthetic locus in B. pertussis. Particularly, conservation of BB3394 in sequenced B. bronchiseptica genomes suggests the importance of this gene for bacteria normal physiology. Deletion of BB3394 abolished resistance to naive serum as described for other LPS mutants. When purified LPS was analyzed, differences in the LPS core structure were found. Particularly, a GalNA branched sugar substitution in the core was absent in the LPS obtained from BB3394 deletion mutant. Absence of GalNA in core LPS alters immune response in vivo but is able to induce protective response against B. bronchiseptica infection. © 2016

Registro:

Documento: Artículo
Título:Modifications of Bordetella bronchiseptica core lipopolysaccharide influence immune response without affecting protective activity
Autor:Sisti, F.; Fernández, J.; Cordero, A.; Casabuono, A.; Couto, A.; Hozbor, D.
Filiación:Instituto de Biotecnología y Biología Molecular, CCT La Plata CONICET, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115, La Plata, 1900, Argentina
CIHIDECAR, Departamento de Química Orgánica, Facultad de Cs Exactas y Naturales, Universidad de Buenos Aires, Bs. As., 1428, Argentina
Palabras clave:Bordetella; Glycosyltransferase; Lipopolysaccharide; bacterium lipopolysaccharide; glycosyltransferase; bacterial protein; lipopolysaccharide; adaptive immunity; animal experiment; Article; bacterial gene; bacterial genome; BB3394 gene; BB3400 gene; Bordetella bronchiseptica; Bordetella bronchiseptica infection; chemical modification; controlled study; deletion mutant; gene locus; genetic conservation; immune response; in vivo study; innate immunity; mouse; nonhuman; animal; biosynthesis; Bordetella bronchiseptica; chemistry; genetics; immune system; immunology; matrix-assisted laser desorption-ionization mass spectrometry; metabolism; Animals; Bacterial Proteins; Bordetella bronchiseptica; Genes, Bacterial; Glycosyltransferases; Immune System; Lipopolysaccharides; Mice; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Año:2017
Volumen:27
Número:3
Página de inicio:432
Página de fin:436
DOI: http://dx.doi.org/10.1016/j.bmcl.2016.12.049
Título revista:Bioorganic and Medicinal Chemistry Letters
Título revista abreviado:Bioorg. Med. Chem. Lett.
ISSN:0960894X
CODEN:BMCLE
CAS:glycosyltransferase, 9033-07-2; Bacterial Proteins; Glycosyltransferases; Lipopolysaccharides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0960894X_v27_n3_p432_Sisti

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Citas:

---------- APA ----------
Sisti, F., Fernández, J., Cordero, A., Casabuono, A., Couto, A. & Hozbor, D. (2017) . Modifications of Bordetella bronchiseptica core lipopolysaccharide influence immune response without affecting protective activity. Bioorganic and Medicinal Chemistry Letters, 27(3), 432-436.
http://dx.doi.org/10.1016/j.bmcl.2016.12.049
---------- CHICAGO ----------
Sisti, F., Fernández, J., Cordero, A., Casabuono, A., Couto, A., Hozbor, D. "Modifications of Bordetella bronchiseptica core lipopolysaccharide influence immune response without affecting protective activity" . Bioorganic and Medicinal Chemistry Letters 27, no. 3 (2017) : 432-436.
http://dx.doi.org/10.1016/j.bmcl.2016.12.049
---------- MLA ----------
Sisti, F., Fernández, J., Cordero, A., Casabuono, A., Couto, A., Hozbor, D. "Modifications of Bordetella bronchiseptica core lipopolysaccharide influence immune response without affecting protective activity" . Bioorganic and Medicinal Chemistry Letters, vol. 27, no. 3, 2017, pp. 432-436.
http://dx.doi.org/10.1016/j.bmcl.2016.12.049
---------- VANCOUVER ----------
Sisti, F., Fernández, J., Cordero, A., Casabuono, A., Couto, A., Hozbor, D. Modifications of Bordetella bronchiseptica core lipopolysaccharide influence immune response without affecting protective activity. Bioorg. Med. Chem. Lett. 2017;27(3):432-436.
http://dx.doi.org/10.1016/j.bmcl.2016.12.049