Abstract:
The catalytic core of a 10-23 DNAzyme was modified using (2′R), (2′S)-2′-deoxy-2′-C-methyluridine and LNA-T. Catalytic activities under pseudo first order conditions were compared at different Mg 2+ concentrations, indicating that certain 2′-C-methyl modified DNAzymes have significant activities. Resistance against MCF-7 cell lysate and endonuclease RQ1 was also measured, showing that the introduction of 2′-C-methyl-2′-deoxynucleosides increased the stability. © 2010 Elsevier Ltd. All rights reserved.
Registro:
Documento: |
Artículo
|
Título: | 10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core |
Autor: | Robaldo, L.; Montserrat, J.M.; Iribarren, A.M. |
Filiación: | INGEBI (CONICET), Vuelta de Obligado 2490-(1428), Buenos Aires, Argentina Instituto de Ciencias, Universidad Nacional de Gral. Sarmiento, J. M. Gutierrez 1150, Los Polvorines (B1613GSX), Prov. de Bs. As., Argentina Laboratorio de Biotransformaciones, Universidad Nacional de Quilmes, Roque Saenz Peña 352 (1876) Bernal, Prov. de Bs. As., Argentina
|
Palabras clave: | 10-23 DNAzyme; 2′-Deoxy-2′-C-methyl nucleosides; 10 23 DNAzyme; 2' deoxy 2' c methyluridine; deoxyribozyme; endonuclease; endonuclease RQ1; magnesium ion; nucleoside analog; unclassified drug; uridine derivative; article; cancer cell culture; cancer resistance; catalysis; cell lysate; controlled study; DNA modification; drug synthesis; enzyme activity; human; human cell; Catalytic Domain; Cell Line, Tumor; DNA, Catalytic; DNA, Single-Stranded; Humans; Magnesium; Uridine |
Año: | 2010
|
Volumen: | 20
|
Número: | 15
|
Página de inicio: | 4367
|
Página de fin: | 4370
|
DOI: |
http://dx.doi.org/10.1016/j.bmcl.2010.06.071 |
Título revista: | Bioorganic and Medicinal Chemistry Letters
|
Título revista abreviado: | Bioorg. Med. Chem. Lett.
|
ISSN: | 0960894X
|
CODEN: | BMCLE
|
CAS: | deoxyribozyme, 206668-15-7; endonuclease, 9055-11-2; magnesium ion, 22537-22-0; 2'-C-methyluridine; DNA, Catalytic; DNA, Single-Stranded; Magnesium, 7439-95-4; RNA-cleaving DNA 10-23; Uridine, 58-96-8
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0960894X_v20_n15_p4367_Robaldo |
Referencias:
- Strobel, S.A., Cochrane, J.C., (2007) Curr. Opin. Chem. Biol., 11, p. 636
- Santoro, S.W., Joyce, G.F., (1997) Proc. Natl. Acad. Sci. U.S.A., 94, p. 4262
- Stoltenburg, R., Reinemann, C., Strehlitz, B., (2007) Biomol. Eng., 24, p. 381
- Corey, D.R., (2007) J. Clin. Invest., 117, p. 3615
- Bhindi, R., Fahmy, R.G., Lowe, H.C., Chesterman, C.N., Dass, C.R., Cairns, M.J., Saravolac, E.G., Kachigian, L.M., (2007) Am. J. Phatol., 171, p. 1079
- Zaborowska, A., Schubert, S., Kurreck, J., Erdmann, V.A., (2005) FEBS Lett., 579, p. 554
- Joyce, G.F., (2001) Methods Enzymol., 341, p. 503
- Nowakowski, J., Shim, P., Prasad, G.S., Stout, C.D., Joyce, G.F., (1999) Nat. Struct. Biol., 6, p. 151
- Nowakowski, J., Shim, P.J., Stout, D., Joyce, G.F., (2000) J. Mol. Biol., 300, p. 93
- Nowakowski, J., Shim, P.J., Joyce, G.F., Stout, C.D., (1999) Acta Crystallogr., Sect. D, 55, p. 1885
- Kenward, M., Dorfman, D.D., (2009) Biophys. J., 97, p. 2785
- Sun, L.-Q., Cairns, M.J., Gerlach, W.L., Witherington, C., Wang, L., King, A., (1999) J. Biol. Chem., 274, p. 17236
- Lu, Z.-X., Ye, M., Yan, G.-R., Li, Q., Tang, M., Lee, L.M., Sun, L.-Q., Cao, Y., (2005) Cancer Gene Ther., 12, p. 647
- Vester, B., Lundberg, L.B., Sorensen, M.D., Babu, B.R., Douthwaite, S., Wengel, J., (2002) J. Am. Chem. Soc., 124, p. 13682
- Schubert, F., Gül, D.C., Grunert, H.-P., Zeichhardt, H., Erdmann, V.A., Kurreck, J., (2003) Nucleic Acids Res., 31, p. 5982
- Reyes-Gutierrez, P., Álvarez-Sala, L.M., (2009) Oligonucleotides, 19, p. 233
- Nawrot, B., Widera, K., Wojcik, M., Rebowska, B., Nowak, G., Stec, W.J., (2007) FEBS J., 274, p. 1062
- Zaborowska, Z., Fürste, J.P., Erdmann, V.A., Kurreck, J., (2002) J. Biol. Chem., 277, p. 40617
- Cicero, D.O., Neuner, P.J.S., Franzese, O., D'Onofrio, C., Iribarren, A.M., (1994) Bioorg. Med. Chem. Lett., 4, p. 861
- Caballero, G., Gallo, M., Montserrat, J.M., Iribarren, A.M., (2002) Helv. Chim. Acta, 85, p. 1284
- Cicero, D.O., Iribarren, A.M., Bazzo, R., (1994) Appl. Magn. Reson., 7, p. 95
- Iribarren, A.M., Cicero, D.O., Neuner, P.J., (1994) Antisense Res. Dev., 4, p. 95
Citas:
---------- APA ----------
Robaldo, L., Montserrat, J.M. & Iribarren, A.M.
(2010)
. 10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core. Bioorganic and Medicinal Chemistry Letters, 20(15), 4367-4370.
http://dx.doi.org/10.1016/j.bmcl.2010.06.071---------- CHICAGO ----------
Robaldo, L., Montserrat, J.M., Iribarren, A.M.
"10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core"
. Bioorganic and Medicinal Chemistry Letters 20, no. 15
(2010) : 4367-4370.
http://dx.doi.org/10.1016/j.bmcl.2010.06.071---------- MLA ----------
Robaldo, L., Montserrat, J.M., Iribarren, A.M.
"10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core"
. Bioorganic and Medicinal Chemistry Letters, vol. 20, no. 15, 2010, pp. 4367-4370.
http://dx.doi.org/10.1016/j.bmcl.2010.06.071---------- VANCOUVER ----------
Robaldo, L., Montserrat, J.M., Iribarren, A.M. 10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core. Bioorg. Med. Chem. Lett. 2010;20(15):4367-4370.
http://dx.doi.org/10.1016/j.bmcl.2010.06.071