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Abstract:

The decolorizing capacity of 26 white rot fungi from Argentina was investigated. Extracellular production of ligninolytic enzymes by mycelium growing on solid malt extract/glucose medium supplemented with different dyes (Malachite Green, Azure B, Poly R-478, Anthraquinone Blue, Congo Red and Xylidine), dye decolorization and the relationship between these two processes were studied. Only ten strains decolorized all the dyes, all ten strains produced laccase, lignin peroxidase and manganese peroxidase on solid medium. However, six of the strains could not decolorize any of the dyes; all six strains tested negative for lignin peroxidase, and produced less than 0.05 U/g agar of manganese peroxidase. Comparing the isolates with the well-known dye-degrader Phanerochaete chrysosporium, a new fungus was identified: Coriolus versicolor f. antarcticus, potentially a candidate for use in biodecoloration processes. Eighteen day-old cultures of this fungus were able to decolorize in an hour 28%, 30%, 43%, 88% and 98% of Xylidine (24 mg/l), Poly R-478 (75 mg/l), Remazol Brilliant Blue R (9 mg/l), Malachite Green (6 mg/l) and Indigo Carmine (23 mg/l), respectively. Laccase activity was 0.13 U/ml, but neither lignin peroxidase nor manganese peroxidase were detected in the extracellular fluids for that day of incubation. © 2003 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Evaluation of Argentinean white rot fungi for their ability to produce lignin-modifying enzymes and decolorize industrial dyes
Autor:Levin, L.; Papinutti, L.; Forchiassin, F.
Filiación:Dpto. de Biodiversidad Y Biol. Exp., Micología Experimental, Universidad de Buenos Aires, Capital Federal 1428, Argentina
Palabras clave:Coriolus versicolor f. antarcticus; Dye decoloration; Ligninolytic enzymes; White rot fungi; Dyes; Enzymes; Fungi; Manganese compounds; Strain; Decolorizing capacity; Extracellular production; Biotechnology; anthraquinone blue; anthraquinone derivative; azure B; brilliant blue; congo red; dye; fungal enzyme; indigo carmine; industrial enzyme; laccase; lignin; lignin peroxidase; malachite green; manganese peroxidase; unclassified drug; dye; article; biotechnology; controlled study; decolorization; enzyme activity; enzyme assay; enzyme synthesis; fungal strain; fungus culture; nonhuman; Phanerochaete; priority journal; reaction time; solid state fermentation; strain difference; Argentina; Basidiomycota; Biodegradation, Environmental; Cereals; Color; Coloring Agents; Industrial Waste; Laccase; Lignin; Peroxidases; Species Specificity; Time Factors; Water Pollutants, Chemical; Chrysosporium; Coriolus versicolor; Fungi; Indigofera; Phanerochaete; Phanerochaete chrysosporium; Siproeta stelenes
Año:2004
Volumen:94
Número:2
Página de inicio:169
Página de fin:176
DOI: http://dx.doi.org/10.1016/j.biortech.2003.12.002
Título revista:Bioresource Technology
Título revista abreviado:Bioresour. Technol.
ISSN:09608524
CODEN:BIRTE
CAS:azure B, 531-55-5; brilliant blue, 2650-18-2; congo red, 573-58-0, 80701-77-5; indigo carmine, 860-22-0; laccase, 80498-15-3; lignin peroxidase, 42613-30-9; lignin, 9005-53-2; malachite green, 569-64-2; manganese peroxidase, 114995-15-2; Coloring Agents; Industrial Waste; Laccase, EC 1.10.3.2; lignin peroxidase, EC 1.11.1.-; Lignin, 9005-53-2; manganese peroxidase, EC 1.11.1.13; Peroxidases, EC 1.11.1.-; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09608524_v94_n2_p169_Levin

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Citas:

---------- APA ----------
Levin, L., Papinutti, L. & Forchiassin, F. (2004) . Evaluation of Argentinean white rot fungi for their ability to produce lignin-modifying enzymes and decolorize industrial dyes. Bioresource Technology, 94(2), 169-176.
http://dx.doi.org/10.1016/j.biortech.2003.12.002
---------- CHICAGO ----------
Levin, L., Papinutti, L., Forchiassin, F. "Evaluation of Argentinean white rot fungi for their ability to produce lignin-modifying enzymes and decolorize industrial dyes" . Bioresource Technology 94, no. 2 (2004) : 169-176.
http://dx.doi.org/10.1016/j.biortech.2003.12.002
---------- MLA ----------
Levin, L., Papinutti, L., Forchiassin, F. "Evaluation of Argentinean white rot fungi for their ability to produce lignin-modifying enzymes and decolorize industrial dyes" . Bioresource Technology, vol. 94, no. 2, 2004, pp. 169-176.
http://dx.doi.org/10.1016/j.biortech.2003.12.002
---------- VANCOUVER ----------
Levin, L., Papinutti, L., Forchiassin, F. Evaluation of Argentinean white rot fungi for their ability to produce lignin-modifying enzymes and decolorize industrial dyes. Bioresour. Technol. 2004;94(2):169-176.
http://dx.doi.org/10.1016/j.biortech.2003.12.002