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Abstract:

Exopolysaccharide (EPS) production by Ganoderma lucidum in response to different culture conditions was studied. Cellulose and glucose, in defined media, resulted in the more efficient enhancers of EPS production among the carbon sources tested. In natural media cultures containing glucose and malt extract exhibited a marked increase (up to 29-fold) respect to defined media. Subsequently, high malt extract and glucose concentrations were tested. G. lucidum produced two fractions of EPS, water-soluble and water-insoluble under these culture conditions. The maximum value (15 g L-1) was reached at 21 days in the medium containing 60 g L-1 malt extract and 40 g L-1 glucose. The incomplete utilization of reducing sugars by the fungus in these media suggested that not only did high malt extract and glucose concentrations play a role in EPS production but also the water activity might be involved. A factorial uniform experimental design to test the effect of malt extract, polyethylene glycol (PEG, as water activity depressor), and initial pH on specific EPS production was applied. G. lucidum showed to be a more efficient specific EPS (mg EPS per g mycelium) producer at pH 3.5 in cultures containing the highest PEG and malt extract concentrations. © 2009 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex
Autor:Papinutti, L.
Filiación:Laboratorio de Micología Experimental, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:Doehlert factorial design; Exopolysaccharide; Ganoderma lucidum; Water activity; Carbon; Enzymes; Extraction; Glucose; Metabolites; pH effects; Culture conditions; Exopolysaccharides; Factorial design; Ganoderma Lucidum; Glucose concentration; Reducing sugars; Water activity; Water potential; Produced Water; carbon; cellulose; exopolysaccharide; glucose; macrogol; nitrogen; glucose; macrogol derivative; polysaccharide; water; biological production; carbon; exopolymer; experimental design; factor analysis; glucose; mushroom; nutrient use; pH; plant extract; plant water relations; polysaccharide; article; carbon source; culture medium; fungal biomass; fungal strain; fungus culture; Ganoderma lucidum; malt; mycelium; nonhuman; nutrient; pH; priority journal; soil water potential; biotechnology; chemistry; fungus; metabolism; methodology; pH; regression analysis; time; wood; Fungi; Ganoderma lucidum; Biotechnology; Carbon; Culture Media; Fungi; Glucose; Hydrogen-Ion Concentration; Polyethylene Glycols; Polysaccharides; Regression Analysis; Reishi; Time Factors; Water; Wood
Año:2010
Volumen:101
Número:6
Página de inicio:1941
Página de fin:1946
DOI: http://dx.doi.org/10.1016/j.biortech.2009.09.076
Título revista:Bioresource Technology
Título revista abreviado:Bioresour. Technol.
ISSN:09608524
CODEN:BIRTE
CAS:carbon, 7440-44-0; cellulose, 61991-22-8, 68073-05-2, 9004-34-6; glucose, 50-99-7, 84778-64-3; macrogol, 25322-68-3; nitrogen, 7727-37-9; water, 7732-18-5; Carbon, 7440-44-0; Culture Media; Glucose, 50-99-7; Polyethylene Glycols; Polysaccharides; Water, 7732-18-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09608524_v101_n6_p1941_Papinutti

Referencias:

  • Bae, J.T., Sim, J.S., Lee, B.C., Pyo, H.B., Choe, T.B., Yun, J.W., Production of exopolysaccharide from mycelial culture of Grifola frondosa and its inhibitory effect on matrix metalloproteinase-1 expression in UV-irradiated human dermal fibroblasts (2005) FEMS Microbiol. Lett., 251, pp. 347-354
  • Chen, W., Zhao, Z., Chen, S.-F., Li, Y.-Q., Optimization for the production of exopolysaccharide from Fomes fomentarius in submerged culture and its antitumor effect in vitro (2008) Bioresour. Technol., 99, pp. 3187-3194
  • Doehlert, D.H., Uniform shell designs (1970) Appl. Stat., 19, pp. 231-239
  • Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., Colorimetric method for determination of sugar and related substances (1945) Anal. Chem., 28, pp. 350-356
  • Gutiérrez, A., Prieto, A., Martínez, A.T., Structural characterization of extracellular polysaccharides produced by fungi from the genus Pleurotus (1996) Carbohydr. Res., 281, pp. 143-154
  • Hseu, R.-S., Wang, H.-H., Wang, H.-F., Moncalvo, J.-M., Differentiation and grouping of isolates of the Ganoderma lucidum complex by random amplified polymorphic DNA-PCR compared with grouping on the basis of internal transcribed Spacer Sequences (1996) Appl. Environ. Microbiol., 62, pp. 1354-1363
  • Hsieh, C., Tseng, M.H., Liu, C.J., Production of polysaccharides from Ganoderma lucidum (CCRC 36041) under limitations of nutrients (2006) Enzyme Microb. Technol., 38, pp. 109-117
  • Jong, S.C., Birmingham, J.M., Medicinal benefits of the mushroom Ganoderma (1992) Adv. Appl. Microbiol., 37, pp. 101-134
  • Kim, H.O., Lim, J.-M., Joo, J.H., Kim, S.W., Hwang, H.J., Choi, J.W., Yun, J.W., Optimization of submerged culture condition for the production of mycelial biomass and exopolysaccharides by Agrocybe cylindracea (2005) Bioresour. Technol., 96, pp. 1175-1182
  • Kim, H.M., Park, M.K., Yun, J.W., Culture pH affects exopolysaccharide production in submerged mycelial culture of Ganoderma lucidum (2006) Appl. Biochem. Biotechnol., 134, pp. 249-262
  • Krisman, C.R., A method for the colorimetric estimation of glycogen with iodine (1962) Anal. Biochem., 4, pp. 17-23
  • Lee, K.M., Lee, S.Y., Lee, H.Y., Bistage control of pH for improving exopolysaccharide production from mycelia of Ganoderma lucidum in an airlift fermentor (1999) J. Biosci. Bioeng., 88, pp. 646-650
  • Lee, W.Y., Park, Y., Ahn, J.K., Ka, K.H., Park, S.Y., Factors influencing the production of endopolysaccharide and exopolysaccharide from Ganoderma applanatum (2007) Enzyme Microb. Technol., 40, pp. 249-254
  • Liang, J., Melican, D., Cafro, L., Palace, G., Fisette, R., Armstrong, R., Patchen, M.L., Enhanced clearance of a multiple antibiotic resistance Staphylococcus aureus in rats treated with PGG-glucan is associated with increased leukocyte counts and increased neutrophil oxidative burst activity (1998) Int. J. Immunopharmacol., 20, pp. 595-614
  • Lim, J.-M., Yun, J.-W., Enhanced production of exopolysaccharides by supplementation of toluene in submerged culture of an edible mushroom Collybia maculata TG-1 (2006) Process Biochem, 41, pp. 1620-1626
  • Lin, E.-S., Sung, S.-C., Cultivating conditions influence exopolysaccharide production by the edible Basidiomycete Antrodia cinnamomea in submerged culture (2006) Int. J. Food Microbiol., 108, pp. 182-187
  • Lin, Y., Zhang, Z., Thibault, J., Aureobasidium pullulans batch cultivations based on a factorial design for improving the production and molecular weight of exopolysaccharides (2007) Process Biochem, 42, pp. 820-827
  • Maziero, R., Bononi, V.L., Adami, A., Cavazzoni, V., Exopolysaccharide and biomass production in submerged culture by edible mushrooms (1995) Proceedings of the 14th International Congress on the Science and Cultivation of Edible Fungi, pp. 887-891
  • Money, N.P., Osmotic pressure of aqueous polyethylene glycols. Relationship between weight and vapour pressure deficit (1989) Plant Physiol, 91, pp. 766-769
  • Pohkrel, C.P., Ohga, S., Submerged culture conditions for mycelial yield and polysaccharides production by Lyophyllum decastes (2007) Food Chem, 107, pp. 641-646
  • Ruel, K., Joseleau, J.P., Involvement of an extracellular glucan sheath during degradation of Populus wood by Phanerochaete chrysosporium (1991) Appl. Environ. Microbiol., 57, pp. 374-384
  • Russell, R., Paterson, M., Ganoderma - a therapeutic fungal biofactory (2006) Phytochemistry, 67, pp. 1985-2001
  • Schnider-Keel, U., LejbØlle, K.B., Baehler, E., Haas, D., Keel, C., The sigma factor AlgU (AlgT) controls exopolysaccharide production and tolerance towards desiccation and osmotic stress in the biocontrol agent Pseudomonas fluorescens CHA0 (2001) Appl. Environ. Microbiol., 67, pp. 5683-5693
  • Selbmann, L., Stingele, S., Petruccioli, M., Exopolysaccharide production by filamentous fungi: the example of Botryosphaeria rhodina (2003) Antonie Van Leeuwenhoek, 84, pp. 135-145
  • Shih, I.-L., Chou, B.-W., Chen, C.-C., Wu, J.-Y., Hsieh, C., Study of mycelial growth and bioactive polysaccharide production in batch and fed-batch culture of Grifola frondosa (2008) Bioresour. Technol., 99, pp. 785-793
  • Simon, L., Bouchet, B., Bremond, K., Gallant, D.J., Bouchonneau, M., Studies on pullulan extracellular production and glycogen intracellular content in Aureobasidium pullulans (1998) Can. J. Microbiol., 44, pp. 1193-1199
  • Sone, Y., Okuda, R., Wada, N., Kishida, E., Misaki, A., Structure and antitumor activities of the polysaccharide isolated from fruiting body and the growing culture of mycelium of Ganoderma lucidum (1985) Agric. Biol. Chem., 49, pp. 2641-2653
  • Sutherland, I.W., Extracellular polysaccharides (1996) Biotechnology, 6, pp. 615-657. , Rhem, H. J., Reed, G. (Eds.), VCH, Weinheim
  • Tang, Y.J., Zhong, J.J., Fed-batch fermentation of Ganoderma lucidum for hyperproduction of polysaccharides and ganoderic acid (2002) Enzyme Microb. Technol., 31, pp. 20-28
  • Tzianabos, A.O., Polysaccharide immunomodulators as therapeutic agents: structural aspects and biologic function (2000) Clin. Microbiol. Rev., 13, pp. 523-533
  • Vesentini, D., Dickinson, D.J., Murphy, R.J., The production of extracellular mucilaginous material (ECMM) in two wood-rotting basidiomycetes is affected by growth conditions (2005) Mycologia, 97, pp. 1163-1170
  • Vesentini, D., Dickinson, D.J., Murphy, R.J., The protective role of the extracellular mucilaginous material (ECMM) from two wood-rotting basidiomycetes against copper toxicity (2007) Int. Biodet. Biodegrad., 60, pp. 1-7
  • Wakshull, E., Brunke-Reese, D., Lindermuth, J., Fisette, L., Nathans, R.S., Crowley, J.J., Tufts, J.C., Adams, D.S., PGG-glucan, a soluble β-(1,3)-glucan, enhances the oxidative burst response, microbicidal activity, and activates an NF-kappa B-like factor in human PMN: evidence for a glycosphingolipid β-(1,3)-glucan receptor (1999) Immunopharmacology, 41, pp. 89-107
  • Zou, X., Sun, M., Guo, X., Quantitative response of cell growth and polysaccharide biosynthesis by the medicinal mushroom Phellinus linteus to NaCl in the medium (2006) World J. Microbiol. Biotechnol., 22, pp. 1129-1133

Citas:

---------- APA ----------
(2010) . Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex. Bioresource Technology, 101(6), 1941-1946.
http://dx.doi.org/10.1016/j.biortech.2009.09.076
---------- CHICAGO ----------
Papinutti, L. "Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex" . Bioresource Technology 101, no. 6 (2010) : 1941-1946.
http://dx.doi.org/10.1016/j.biortech.2009.09.076
---------- MLA ----------
Papinutti, L. "Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex" . Bioresource Technology, vol. 101, no. 6, 2010, pp. 1941-1946.
http://dx.doi.org/10.1016/j.biortech.2009.09.076
---------- VANCOUVER ----------
Papinutti, L. Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex. Bioresour. Technol. 2010;101(6):1941-1946.
http://dx.doi.org/10.1016/j.biortech.2009.09.076