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Abstract:

Milled summer thalli of Gracilaria gracilis from Argentina were sequentially extracted with water at room temperature (RTW1-3), 70 °C (W701-3) and 90 °C (W901-2). Both W701 and W901 consisted of high molecular weight polysaccharides (ca. 540,000 Da), but polydispersity was higher for the major product W701 (yield, 72% of the recovered). Structural analyzes by methylation and 13C NMR spectroscopy revealed that W701 was mainly agarose. Alkaline treatment, together with structural analyzes, indicated a negligible proportion of precursor l-galactose 6-sulfate residues in this product, while they were clearly detected in the 13C NMR spectra of RTW2-3. The presence of floridean starch in W901 had an antagonistic effect on its gel strength, which resulted nearly three times lower than that of fraction W701. Ultrastructural observation by transmission electron microscopy showed that, after extraction with hot water, a partial loss of cell wall stratification and disorganization of the cuticle had occurred. Final cellular debris exhibited swelling in the microfibrillar component. After this first thorough study of the chemical composition and physical properties of the products of G. gracilis from Bahía Bustamante we conclude that a good quality agarose is obtained in high yield after extraction with water at 70 °C without the requirement of alkaline pretreatment, which usually produces degradation of the polysaccharide. © 2008 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina - Content, structure and physical properties
Autor:Rodríguez, M.C.; Matulewicz, M.C.; Noseda, M.D.; Ducatti, D.R.B.; Leonardi, P.I.
Filiación:Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Departamento de Química Orgánica-CIHIDECAR (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box 19046, CEP 81531-990 Curitiba, Parana, Brazil
Laboratorio de Ficología y Micología, Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, San Juan 670, 8000 Bahía Blanca, Argentina
Palabras clave:Agarose; Cell wall; Gracilaria gracilis; Structure determination; Sulfated galactans; Biopolymers; Chemical properties; Colloids; Debris; Gelation; Microscopic examination; Molecular weight; Nuclear magnetic resonance; Nuclear magnetic resonance spectroscopy; Photodegradation; Polydispersity; Polysaccharides; Solvent extraction; Walls (structural partitions); Water; Agarose; Cell wall; Gracilaria gracilis; Structure determination; Sulfated galactans; Extraction; agar; agarose; hot water; polysaccharide; sulfate; agar; carbon isotope; chemical composition; extraction method; methylation; nuclear magnetic resonance; physical property; red alga; starch; ultrastructure; article; carbon nuclear magnetic resonance; cell wall; chemical composition; dispersion; extraction; Gracilaria; gracilaria gracilis; methylation; molecular weight; nonhuman; priority journal; qualitative analysis; room temperature; stratification; structure analysis; transmission electron microscopy; ultrastructure; Agar; Argentina; Elasticity; Gracilaria; Hardness; Molecular Weight; Viscosity; Gracilaria gracilis; Gracilariales; Rhodophyta
Año:2009
Volumen:100
Número:3
Página de inicio:1435
Página de fin:1441
DOI: http://dx.doi.org/10.1016/j.biortech.2008.08.025
Título revista:Bioresource Technology
Título revista abreviado:Bioresour. Technol.
ISSN:09608524
CODEN:BIRTE
CAS:agar, 9002-18-0; agarose, 9012-36-6; sulfate, 14808-79-8; Agar, 9002-18-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09608524_v100_n3_p1435_Rodriguez

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Citas:

---------- APA ----------
Rodríguez, M.C., Matulewicz, M.C., Noseda, M.D., Ducatti, D.R.B. & Leonardi, P.I. (2009) . Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina - Content, structure and physical properties. Bioresource Technology, 100(3), 1435-1441.
http://dx.doi.org/10.1016/j.biortech.2008.08.025
---------- CHICAGO ----------
Rodríguez, M.C., Matulewicz, M.C., Noseda, M.D., Ducatti, D.R.B., Leonardi, P.I. "Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina - Content, structure and physical properties" . Bioresource Technology 100, no. 3 (2009) : 1435-1441.
http://dx.doi.org/10.1016/j.biortech.2008.08.025
---------- MLA ----------
Rodríguez, M.C., Matulewicz, M.C., Noseda, M.D., Ducatti, D.R.B., Leonardi, P.I. "Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina - Content, structure and physical properties" . Bioresource Technology, vol. 100, no. 3, 2009, pp. 1435-1441.
http://dx.doi.org/10.1016/j.biortech.2008.08.025
---------- VANCOUVER ----------
Rodríguez, M.C., Matulewicz, M.C., Noseda, M.D., Ducatti, D.R.B., Leonardi, P.I. Agar from Gracilaria gracilis (Gracilariales, Rhodophyta) of the Patagonic coast of Argentina - Content, structure and physical properties. Bioresour. Technol. 2009;100(3):1435-1441.
http://dx.doi.org/10.1016/j.biortech.2008.08.025