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Abstract:

Two aspects of light are very important for plant development: the length of the light phase or photoperiod and the quality of incoming light. Photoperiod detection allows plants to anticipate the arrival of the next season, whereas light quality, mainly the red to far-red ratio (R:FR), is an early signal of competition by neighbouring plants. phyB represses flowering by antagonising CO at the transcriptional and post-translational levels. A low R:FR decreases active phyB and consequently increases active CO, which in turn activates the expression of FT, the plant florigen. Other phytochromes like phyD and phyE seem to have redundant roles with phyB. PFT1, the MED25 subunit of the plant Mediator complex, has been proposed to act in the light-quality pathway that regulates flowering time downstream of phyB. However, whether PFT1 signals through CO and its specific mechanism are unclear. Here we show that CO-dependent and-independent mechanisms operate downstream of phyB, phyD and phyE to promote flowering, and that PFT1 is equally able to promote flowering by modulating both CO-dependent and-independent pathways. Our data are consistent with the role of PFT1 as an activator of CO transcription, and also of FT transcription, in a CO-independent manner. Our transcriptome analysis is also consistent with CO and FT genes being the most important flowering targets of PFT1. Furthermore, comparison of the pft1 transcriptome with transcriptomes after fungal and herbivore attack strongly suggests that PFT1 acts as a hub, integrating a variety of interdependent environmental stimuli, including light quality and jasmonic acid-dependent defences. © 2011 Blackwell Publishing Ltd.

Registro:

Documento: Artículo
Título:PFT1, the MED25 subunit of the plant Mediator complex, promotes flowering through CONSTANS dependent and independent mechanisms in Arabidopsis
Autor:Iñigo, S.; Alvarez, M.J.; Strasser, B.; Califano, A.; Cerdán, P.D.
Filiación:Fundación Instituto Leloir, IIBBA-CONICET, Buenos Aires, Argentina
Universidad Nacional de Quilmes, Bernal, B1876BXD, Argentina
Center for Computational Biology and Bioinformatics (C2B2), Columbia University, 1130 St. Nicholas Ave, New York, NY 10032, United States
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1405BWE-Buenos Aires, Argentina
Palabras clave:Arabidopsis; CONSTANS; flowering; jasmonate; PFT1; photoperiod; Arabidopsis; CONSTANS; flowering; Jasmonates; PFT1; photoperiod; Genes; Plants (botany); Transcription; apoprotein; Arabidopsis protein; CONSTANS protein, Arabidopsis; cyclopentane derivative; DNA binding protein; FT protein, Arabidopsis; jasmonic acid; mediator complex; nuclear protein; oxylipin; PFT1 protein, Arabidopsis; PHYB protein, Arabidopsis; PHYD protein, Arabidopsis; PHYE protein, Arabidopsis; phytochrome; phytochrome B; transcription factor; transcriptome; animal; Arabidopsis; article; biological model; Botrytis; butterfly; flower; Fusarium; gene expression regulation; genetics; light; metabolism; mutation; photoperiodicity; physiology; plant leaf; radiation exposure; seedling; signal transduction; temperature; Thysanoptera; Animals; Apoproteins; Arabidopsis; Arabidopsis Proteins; Botrytis; Butterflies; Cyclopentanes; DNA-Binding Proteins; Flowers; Fusarium; Gene Expression Regulation, Plant; Light; Mediator Complex; Models, Biological; Mutation; Nuclear Proteins; Oxylipins; Photoperiod; Phytochrome; Phytochrome B; Plant Leaves; Seedling; Signal Transduction; Temperature; Thysanoptera; Transcription Factors; Transcriptome; Arabidopsis
Año:2012
Volumen:69
Número:4
Página de inicio:601
Página de fin:612
DOI: http://dx.doi.org/10.1111/j.1365-313X.2011.04815.x
Título revista:Plant Journal
Título revista abreviado:Plant J.
ISSN:09607412
CODEN:PLJUE
CAS:jasmonic acid, 6894-38-8; phytochrome, 117102-58-6; Apoproteins; Arabidopsis Proteins; CONSTANS protein, Arabidopsis; Cyclopentanes; DNA-Binding Proteins; FT protein, Arabidopsis; Mediator Complex; Nuclear Proteins; Oxylipins; PFT1 protein, Arabidopsis; PHYB protein, Arabidopsis; PHYD protein, Arabidopsis, 158379-16-9; PHYE protein, Arabidopsis; Phytochrome, 11121-56-5; Phytochrome B, 136250-22-1; Transcription Factors; jasmonic acid, 6RI5N05OWW
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09607412_v69_n4_p601_Inigo

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Citas:

---------- APA ----------
Iñigo, S., Alvarez, M.J., Strasser, B., Califano, A. & Cerdán, P.D. (2012) . PFT1, the MED25 subunit of the plant Mediator complex, promotes flowering through CONSTANS dependent and independent mechanisms in Arabidopsis. Plant Journal, 69(4), 601-612.
http://dx.doi.org/10.1111/j.1365-313X.2011.04815.x
---------- CHICAGO ----------
Iñigo, S., Alvarez, M.J., Strasser, B., Califano, A., Cerdán, P.D. "PFT1, the MED25 subunit of the plant Mediator complex, promotes flowering through CONSTANS dependent and independent mechanisms in Arabidopsis" . Plant Journal 69, no. 4 (2012) : 601-612.
http://dx.doi.org/10.1111/j.1365-313X.2011.04815.x
---------- MLA ----------
Iñigo, S., Alvarez, M.J., Strasser, B., Califano, A., Cerdán, P.D. "PFT1, the MED25 subunit of the plant Mediator complex, promotes flowering through CONSTANS dependent and independent mechanisms in Arabidopsis" . Plant Journal, vol. 69, no. 4, 2012, pp. 601-612.
http://dx.doi.org/10.1111/j.1365-313X.2011.04815.x
---------- VANCOUVER ----------
Iñigo, S., Alvarez, M.J., Strasser, B., Califano, A., Cerdán, P.D. PFT1, the MED25 subunit of the plant Mediator complex, promotes flowering through CONSTANS dependent and independent mechanisms in Arabidopsis. Plant J. 2012;69(4):601-612.
http://dx.doi.org/10.1111/j.1365-313X.2011.04815.x