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In plant sexual reproduction, water and solute movement are tightly regulated, suggesting the involvement of aquaporins. We previously identified TIP5;1 and TIP1;3 as the only Arabidopsis aquaporin genes that are selectively and highly expressed in mature pollen, and showed that they can transport both water and urea when expressed in Xenopus oocytes. Here, we show that TIP5;1 has unusual characteristics, as its water transport activity is regulated by pH. Analysis of the water transport activity of a mutant version of TIP5;1 (TIP5;1-H131A) and amino acid alignment with other plant aquaporins regulated by pH suggested that a conserved motif is involved in pH sensing. GFP-TIP5;1 is located in the mitochondria of pollen tubes. The single mutants tip1;3 and tip5;1, as well as the tip1;3 tip5;1 double mutant, are fertile, but all mutants had shorter than normal pollen tubes when germinated in vitro in the absence of exogenous nitrogen. Thus, we propose that TIP5;1 and TIP1;3 are involved in nitrogen recycling in pollen tubes of Arabidopsis thaliana. © 2010 The Authors. The Plant Journal © 2010 Blackwell Publishing Ltd.


Documento: Artículo
Título:TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana
Autor:Soto, G.; Fox, R.; Ayub, N.; Alleva, K.; Guaimas, F.; Erijman, E.J.; Mazzella, A.; Amodeo, G.; Muschietti, J.
Filiación:Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490 Piso 2, 1428 Buenos Aires, Argentina
Instituto de Genética 'Ewald A. Favret', Centro de Investigaciõn en Ciencias Veterinarias y Agronõmicas (CICVyA), Instituto Nacional de Tecnología Agropecuaria (INTA), Castelar, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:aquaporins; mitochondria; nitrogen metabolism; pH sensing; urea; Aquaporins; Arabidopsis; Arabidopsis thaliana; Double mutants; In-vitro; nitrogen metabolism; pH sensing; Plant sexual reproduction; Pollen tube; Remobilization; Single mutant; Solute movement; Water transport; Xenopus oocyte; Amino acids; Glycoproteins; Metabolism; Mitochondria; pH sensors; Urea; Nitrogen; aquaporin; Arabidopsis protein; nitrogen; plant RNA; TIP5;1 protein, Arabidopsis; water; Arabidopsis; article; gene expression regulation; genetics; metabolism; mitochondrion; pH; phylogeny; pollen tube; Aquaporins; Arabidopsis; Arabidopsis Proteins; Hydrogen-Ion Concentration; Mitochondria; Mutagenesis, Insertional; Nitrogen; Phylogeny; Pollen Tube; RNA, Plant; Water; Arabidopsis; Arabidopsis thaliana
Página de inicio:1038
Página de fin:1047
Título revista:Plant Journal
Título revista abreviado:Plant J.
CAS:aquaporin, 215587-75-0; nitrogen, 7727-37-9; water, 7732-18-5; Aquaporins; Arabidopsis Proteins; Nitrogen, 7727-37-9; RNA, Plant; TIP5;1 protein, Arabidopsis; Water, 7732-18-5


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---------- APA ----------
Soto, G., Fox, R., Ayub, N., Alleva, K., Guaimas, F., Erijman, E.J., Mazzella, A.,..., Muschietti, J. (2010) . TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana. Plant Journal, 64(6), 1038-1047.
---------- CHICAGO ----------
Soto, G., Fox, R., Ayub, N., Alleva, K., Guaimas, F., Erijman, E.J., et al. "TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana" . Plant Journal 64, no. 6 (2010) : 1038-1047.
---------- MLA ----------
Soto, G., Fox, R., Ayub, N., Alleva, K., Guaimas, F., Erijman, E.J., et al. "TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana" . Plant Journal, vol. 64, no. 6, 2010, pp. 1038-1047.
---------- VANCOUVER ----------
Soto, G., Fox, R., Ayub, N., Alleva, K., Guaimas, F., Erijman, E.J., et al. TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana. Plant J. 2010;64(6):1038-1047.