Artículo

Kaothien, P.; Sung, H.O.; Shuai, B.; Wengier, D.; Cotter, R.; Kelley, D.; Kiriakopolos, S.; Muschietti, J.; McCormick, S. "Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth" (2005) Plant Journal. 42(4):492-503
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Abstract:

The pollen-specific receptor kinases LePRK1 and LePRK2 have localization and expression profiles that strongly suggest they play roles in pollen germination and tube growth. To identify downstream components of LePRK signaling, we used their cytoplasmic domains (CDs) as baits in yeast two-hybrid screens of a tomato pollen cDNA library. A pollen-specific protein we named kinase partner protein (KPP) interacted with the CDs of both LePRK1 and LePRK2 in yeast and in an in vitro pull-down assay, and with LePRK2 in a co-immunoprecipitation assay. KPP is a peripheral membrane protein and is phosphorylated in pollen. Pollen tubes over-expressing KPP developed balloon-like tips with abnormal cytoplasmic streaming and F-actin arrangements and plants over-expressing KPP exhibited impaired transmission of the transgene through the male. KPP-like genes are found only in plants; the 14 family members in Arabidopsis thaliana exhibit diverse expression patterns and potentially play roles in signaling pathways in other tissues. © 2005 Blackwell Publishing Ltd.

Registro:

Documento: Artículo
Título:Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth
Autor:Kaothien, P.; Sung, H.O.; Shuai, B.; Wengier, D.; Cotter, R.; Kelley, D.; Kiriakopolos, S.; Muschietti, J.; McCormick, S.
Filiación:Plant Gene Expression Center, United States Department of Agriculture, Agricultural Research Service, Albany, CA 94710, United States
Department of Plant and Microbial Biology, University of California at Berkeley, 800 Buchanan Street, Albany, CA 94710, United States
Departamento de Fisiología, Biología Molecular Y Celular, Instituto de Ingeniería Genética Y Biología Molecular, Universidad de Buenos Aires, Obligado 2490, 1428 Buenos Aires, Argentina
School of Life Science and Biotechnology, Korea University, Seoul 136-701, South Korea
Xoma (US) LLC, 2910 7th St, Berkeley, CA 94710, United States
Palabras clave:Actin; Cytoplasmic streaming; Signaling; Tomato; Vacuole; Yeast two-hybrid; Bioassay; Cytology; Enzymes; Growth kinetics; Proteins; Yeast; Cytoplasmic domains (CD); Kinase partner protein (KPP); Pollen germination; Receptor kinases; Enzyme kinetics; protein kinase; vegetable protein; article; enzymology; flower; gene expression; growth, development and aging; metabolism; molecular genetics; nucleotide sequence; protein binding; protein tertiary structure; signal transduction; tomato; transgenic plant; two hybrid system; Flowers; Gene Expression; Lycopersicon esculentum; Molecular Sequence Data; Plant Proteins; Plants, Genetically Modified; Protein Binding; Protein Kinases; Protein Structure, Tertiary; Signal Transduction; Two-Hybrid System Techniques; Biological Tests; Cytology; Enzymatic Activity; Enzymes; Germination; Growth Rate; Pollen; Proteins; Yeasts; Arabidopsis; Arabidopsis thaliana; Lycopersicon esculentum
Año:2005
Volumen:42
Número:4
Página de inicio:492
Página de fin:503
DOI: http://dx.doi.org/10.1111/j.1365-313X.2005.02388.x
Título revista:Plant Journal
Título revista abreviado:Plant J.
ISSN:09607412
CODEN:PLJUE
CAS:protein kinase, 9026-43-1; Plant Proteins; Protein Kinases, EC 2.7.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09607412_v42_n4_p492_Kaothien

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Citas:

---------- APA ----------
Kaothien, P., Sung, H.O., Shuai, B., Wengier, D., Cotter, R., Kelley, D., Kiriakopolos, S.,..., McCormick, S. (2005) . Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth. Plant Journal, 42(4), 492-503.
http://dx.doi.org/10.1111/j.1365-313X.2005.02388.x
---------- CHICAGO ----------
Kaothien, P., Sung, H.O., Shuai, B., Wengier, D., Cotter, R., Kelley, D., et al. "Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth" . Plant Journal 42, no. 4 (2005) : 492-503.
http://dx.doi.org/10.1111/j.1365-313X.2005.02388.x
---------- MLA ----------
Kaothien, P., Sung, H.O., Shuai, B., Wengier, D., Cotter, R., Kelley, D., et al. "Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth" . Plant Journal, vol. 42, no. 4, 2005, pp. 492-503.
http://dx.doi.org/10.1111/j.1365-313X.2005.02388.x
---------- VANCOUVER ----------
Kaothien, P., Sung, H.O., Shuai, B., Wengier, D., Cotter, R., Kelley, D., et al. Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth. Plant J. 2005;42(4):492-503.
http://dx.doi.org/10.1111/j.1365-313X.2005.02388.x