Artículo
Gualano, N.; Carrari, F.; Rodríguez, M.V.; Pérez-Flores, L.; Sánchez, R.; Iusem, N.; Benech-Arnold, R. "Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling" (2007) Seed Science Research. 17(2):81-90
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Abstract:
In the work reported in this paper, we attempted to elucidate the nature of the different abscisic acid (ABA) sensitivities presented by developing embryos from sorghum [Sorghum bicolor (L.) Moench] lines with contrasting pre-harvest sprouting (PHS) behaviour (Redland B2, susceptible; IS 9530, resistant). We explored two different hypotheses for a possible mechanism: (1) a different functionality of the ABA signalling pathway, and (2) a different rate of ABA degradation/conjugation in the apoplast of embryos from these genotypes. To assess the first possibility, we used an ABA-responsive gene (Rab17) as a reporter of changes in endogenous ABA content, which were artificially induced in embryos from both genotypes by means of fluridone application immediately after anthesis, to reduce ABA content, and embryo incubation in the presence of ABA. A defect in ABA signalling should be seen as a level of Rab17 expression that is independent of endogenous ABA content. For testing the second possibility at two stages of development, embryos from both lines were isolated and incubated in water for different periods. ABA concentrations in embryos and the incubation media were quantified through radioimmunoassay. In contrast to our findings for the resistant IS 9530 line, Rab17 expression did not respond to changes in ABA levels in sensitive Redland B2 embryos. The ABA degradation/conjugation rates in embryos and incubation media did not show clear differences between sorghum lines for any of the developmental stages analysed. These results suggest that a disruption in the ABA signal transduction pathway in Redland B2 underlies the low ABA sensitivity shown by embryos from this line. © 2007 Cambridge University Press.
Registro:
| Documento: | Artículo |
| Título: | Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling |
| Autor: | Gualano, N.; Carrari, F.; Rodríguez, M.V.; Pérez-Flores, L.; Sánchez, R.; Iusem, N.; Benech-Arnold, R. |
| Filiación: | Cátedra de Cerealicultura-IFEVA, Facultad de Agronomía, CONICET, Av. San Martín 4453, (C1417DSE), Buenos Aires, Argentina INTA Castelar, CONICET, Argentina Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana-Iztapalapa, D.F., México, Mexico Cátedra de Fisiología Vegetal, IFEVA, Universidad de Buenos Aires, Argentina Laboratorio de Fisiología Y Biología Molecular, FCEyN, Ciudad Universitaria, Argentina |
| Palabras clave: | Abscisic acid; Abscisic acid sensitivity; Abscisic acid signalling; Germination; Pre-harvest sprouting; Seed dormancy; Sorghum bicolor; Sorghum bicolor |
| Año: | 2007 |
| Volumen: | 17 |
| Número: | 2 |
| Página de inicio: | 81 |
| Página de fin: | 90 |
| DOI: | http://dx.doi.org/10.1017/S0960258507708115 |
| Título revista: | Seed Science Research |
| Título revista abreviado: | Seed Sci. Res. |
| ISSN: | 09602585 |
| CODEN: | SESRE |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09602585_v17_n2_p81_Gualano |
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Citas:
---------- APA ----------
Gualano, N., Carrari, F., Rodríguez, M.V., Pérez-Flores, L., Sánchez, R., Iusem, N. & Benech-Arnold, R. (2007) . Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling. Seed Science Research, 17(2), 81-90.http://dx.doi.org/10.1017/S0960258507708115
---------- CHICAGO ----------
Gualano, N., Carrari, F., Rodríguez, M.V., Pérez-Flores, L., Sánchez, R., Iusem, N., et al. "Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling" . Seed Science Research 17, no. 2 (2007) : 81-90.http://dx.doi.org/10.1017/S0960258507708115
---------- MLA ----------
Gualano, N., Carrari, F., Rodríguez, M.V., Pérez-Flores, L., Sánchez, R., Iusem, N., et al. "Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling" . Seed Science Research, vol. 17, no. 2, 2007, pp. 81-90.http://dx.doi.org/10.1017/S0960258507708115
---------- VANCOUVER ----------
Gualano, N., Carrari, F., Rodríguez, M.V., Pérez-Flores, L., Sánchez, R., Iusem, N., et al. Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling. Seed Sci. Res. 2007;17(2):81-90.http://dx.doi.org/10.1017/S0960258507708115
