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Abstract:

Phytochrome-induced softening of the micropylar endosperm of Datura ferox seeds is followed by radicle protrusion and preceded by a significant increase in the extractable activity of β-mannanase and β-mannosidase (6 and 3 times over the far-red light controls, respectively) and a decrease in the mannan content of the micropylar endosperm cell walls. This relationship between phytochrome-induced germination, endosperm softening and mannan degradation was studied in Datura ferox seeds subjected to a range of water potentials during the presence of Pfr. Low water potential inhibited the decrease in mannose-rich cell-wall insoluble polysaccharides, the increase in activity of β-mannosidase and endosperm softening. A good correlation was found between activity of β-mannosidase measured at 45 h after exposure to a red light pulse and germination counted 24 h later when, with different external water potentials, germination varied between 0 and 90%. In contrast, no inhibitory effect of low water potential on in vitro β-mannanase activity was detected. We suggest that the in vivo action of β-mannanase might be limited by accumulation of manno-oligosaccharides caused by inhibition of β-mannosidase. These data are consistent with the concept of a link between mannan mobilization and endosperm softening. Part of the effect of low water potential on Pfr-induced germination may be mediated by inhibition of β-mannosidase activity, with consequences for mannan hydrolysis and endosperm softening.

Registro:

Documento: Artículo
Título:Effect of low water potential on phytochrome-induced germination, endosperm softening and cell-wall mannan degradation in Datura ferox seeds
Autor:Sánchez, R.A.; De Miguel, L.; Lima, C.; De Lederkremer, R.M.
Filiación:IFEVA, Departamento de Biología Aplicada y Alimentos, Facultad de Agronomía-CONICET, Avda. San martín 4453, Buenos Aires 1417, Argentina
CIHIDECAR, Departamento de Química Orgánica, Universidad de Buenos Aires, Avda. San martín 4453, Buenos Aires 1417, Argentina
Palabras clave:β-mannanase; β-mannosidase; Dormancy; Endosperm softening; Germination; Mannan mobilization; Phytochrome; Water potential; Datura; Datura ferox
Año:2002
Volumen:12
Número:3
Página de inicio:155
Página de fin:163
DOI: http://dx.doi.org/10.1079/SSR2002107
Título revista:Seed Science Research
Título revista abreviado:Seed Sci. Res.
ISSN:09602585
CODEN:SESRE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09602585_v12_n3_p155_Sanchez

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Citas:

---------- APA ----------
Sánchez, R.A., De Miguel, L., Lima, C. & De Lederkremer, R.M. (2002) . Effect of low water potential on phytochrome-induced germination, endosperm softening and cell-wall mannan degradation in Datura ferox seeds. Seed Science Research, 12(3), 155-163.
http://dx.doi.org/10.1079/SSR2002107
---------- CHICAGO ----------
Sánchez, R.A., De Miguel, L., Lima, C., De Lederkremer, R.M. "Effect of low water potential on phytochrome-induced germination, endosperm softening and cell-wall mannan degradation in Datura ferox seeds" . Seed Science Research 12, no. 3 (2002) : 155-163.
http://dx.doi.org/10.1079/SSR2002107
---------- MLA ----------
Sánchez, R.A., De Miguel, L., Lima, C., De Lederkremer, R.M. "Effect of low water potential on phytochrome-induced germination, endosperm softening and cell-wall mannan degradation in Datura ferox seeds" . Seed Science Research, vol. 12, no. 3, 2002, pp. 155-163.
http://dx.doi.org/10.1079/SSR2002107
---------- VANCOUVER ----------
Sánchez, R.A., De Miguel, L., Lima, C., De Lederkremer, R.M. Effect of low water potential on phytochrome-induced germination, endosperm softening and cell-wall mannan degradation in Datura ferox seeds. Seed Sci. Res. 2002;12(3):155-163.
http://dx.doi.org/10.1079/SSR2002107