Artículo

Canosa, L.F.; Pozzi, A.G.; Rosemblit, C.; Ceballos, N.R. "Steroid production in toads" (2003) Journal of Steroid Biochemistry and Molecular Biology. 85(2-5):227-233
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Abstract:

In Bufo arenarum, androgen biosynthesis occurs through a complete 5-ene pathway, including 5-androstane-3β,17β-diol as the immediate precursor of testosterone. Besides, steroidogenesis changes during the breeding period, turning from androgens to C21-steroids such as 5α-pregnan-3α,20α-diol, 3α-hydroxy-5α-pregnan-20-one and 5α-pregnan-3,20-dione. In B. arenarum, steroid hormones are not involved in hCG-induced spermiation, suggesting that the steroidogenic shift to C21-steroids during the breeding be not related to spermiation. The activity of 17-hydroxylase-C 17-20 lyase (CypP450c17) decreases during the reproductive season, suggesting that this enzyme would represent a key enzyme in the regulation of seasonal changes. However, the increase in the affinity for pregnenolone of 3β-hydroxysteroid dehydrogenase (3αHSD)/ isomerase could also be involved. Moreover, the reduction in CypP450 c17 leading to a reduction in C19-steroids, among them dehydroepiandrosterone (DHE), would contribute to the conversion of pregnenolone into progesterone, avoiding the non-competitive inhibition exerted by DHE on this transformation. Additionally, CypP450c17 possesses a higher affinity for pregnenolone than for progesterone, explaining the predominance of the 5-ene pathway for testosterone biosynthesis. Animals in reproductive condition showed a significant reduction in circulating androgens, enhancing the physiological relevance of all the in vitro results. The in vitro effects of mGnRH and hrFSH on testicular steroidogenesis revealed that both hormones inhibited CypP450c17 activity. In summary, these results demonstrate that, in B. arenarum, the change in testicular steroidogenesis during the reproductive period could be partially due to an FSH and GnRH-induced decrease in CypP450c17 activity. © 2003 Elsevier Science Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Steroid production in toads
Autor:Canosa, L.F.; Pozzi, A.G.; Rosemblit, C.; Ceballos, N.R.
Filiación:PRHOM-CONICET, Lab. de Endocrinologia Comparada, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
Palabras clave:Amphibians; GnRH; Steroidogenic-enzymes; Testis; 17 hydroxy 5 pregnen 3,20 dione; 20 hydroxy 5alpha pregnan 3 one; 3(or 17)beta hydroxysteroid dehydrogenase; 3alpha hydroxy 5alpha pregnan 20 one; 3beta androstanediol; 5alpha pregnane 3,20 dione; 5alpha pregnane 3alpha,20alpha diol; androgen; androstanedione; androstanolone; androstenediol; androsterone; chorionic gonadotropin; cytochrome P450; prasterone; pregnenolone; progesterone; steroid; steroid 17,20 lyase; steroid 17alpha monooxygenase; steroid hormone; testosterone; unclassified drug; androgen synthesis; breeding; conference paper; enzyme activity; enzyme substrate; nonhuman; reduction; regulatory mechanism; reproduction; seasonal variation; steroidogenesis; testis; testosterone synthesis; toad; Amphibia; Animalia; Anura; Bufo; Bufo arenarum
Año:2003
Volumen:85
Número:2-5
Página de inicio:227
Página de fin:233
DOI: http://dx.doi.org/10.1016/S0960-0760(03)00234-6
Título revista:Journal of Steroid Biochemistry and Molecular Biology
Título revista abreviado:J. Steroid Biochem. Mol. Biol.
ISSN:09600760
CODEN:JSBBE
CAS:3(or 17)beta hydroxysteroid dehydrogenase, 9015-81-0; 5alpha pregnane 3,20 dione, 566-65-4; 5alpha pregnane 3alpha,20alpha diol, 566-58-5; androstanedione, 5982-99-0, 846-46-8; androstanolone, 521-18-6; androstenediol, 28652-91-7, 521-17-5; androsterone, 53-41-8; chorionic gonadotropin, 9002-61-3; cytochrome P450, 9035-51-2; prasterone, 53-43-0; pregnenolone, 145-13-1; progesterone, 57-83-0; steroid 17alpha monooxygenase, 189355-97-3, 9029-67-8, 9044-50-2, 331947-43-4, 9068-00-2; testosterone, 58-22-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v85_n2-5_p227_Canosa

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Citas:

---------- APA ----------
Canosa, L.F., Pozzi, A.G., Rosemblit, C. & Ceballos, N.R. (2003) . Steroid production in toads. Journal of Steroid Biochemistry and Molecular Biology, 85(2-5), 227-233.
http://dx.doi.org/10.1016/S0960-0760(03)00234-6
---------- CHICAGO ----------
Canosa, L.F., Pozzi, A.G., Rosemblit, C., Ceballos, N.R. "Steroid production in toads" . Journal of Steroid Biochemistry and Molecular Biology 85, no. 2-5 (2003) : 227-233.
http://dx.doi.org/10.1016/S0960-0760(03)00234-6
---------- MLA ----------
Canosa, L.F., Pozzi, A.G., Rosemblit, C., Ceballos, N.R. "Steroid production in toads" . Journal of Steroid Biochemistry and Molecular Biology, vol. 85, no. 2-5, 2003, pp. 227-233.
http://dx.doi.org/10.1016/S0960-0760(03)00234-6
---------- VANCOUVER ----------
Canosa, L.F., Pozzi, A.G., Rosemblit, C., Ceballos, N.R. Steroid production in toads. J. Steroid Biochem. Mol. Biol. 2003;85(2-5):227-233.
http://dx.doi.org/10.1016/S0960-0760(03)00234-6