Artículo

La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Although follicle-stimulating hormone (FSH) and estrogens are known to be the main physiological stimuli for the development of the ovarian follicle in mammals, their growth-promoting activity has not been clearly established 'in vitro'. Furthermore, experimental evidence indicates that FSH and estradiol can independently inhibit granulosa cell proliferation. The present study was aimed at examining the effect of sex steroids in combination with FSH, on DNA synthesis in rat granulosa cells cultured in completely defined medium. Estradiol and FSH, when added separately, produced a significant inhibition of [3H] thymidine incorporation. In contrast, a combination of a low dose of FSH (20 ng/ml) with estradiol (100 ng/ml) produced a shift in the period of maximal DNA synthesis from 96 to 48 h after plating. Dose response studies showed that estradiol effects were produced at physiological intraovarian concentrations (1-100 ng/ml), whereas the effects of FSH were biphasic, with high doses (200 ng/ml) being inhibitory. A similar biphasic dose response curve was observed with increasing concentrations of a cAMP derivative in the presence of maximally effective doses of either an aromatizable steroid (androstenedione), insulin or insulin-like growth factor I. Non-arematizable androgens (5α-dihydrotestosterone, 5α-androstane 3α-17β diol and androsterone) showed a potency comparable to that of estradiol. The effect of 5α-dihydrotestosterone was completely blocked by a specific antiandrogen (hydroxy-flutamide), indicating that it was mediated by the androgen receptor. The effects of estradiol and androgens were not additive. The interaction between estradiol and FSH was further amplified in the presence of a maximally effective dose of insulin. Data presented herein indicate that both estrogens and androgens are able to elicit a mitogenic response in purified granulosa cells, cultured in a completely defined medium, provided the cells are stimulated by a physiological dose of FSH. These results suggest that, during follicular development, the stimulus for granulosa cell proliferation is given by the concerted action of steroid and peptide hormones acting through different signalling pathways.

Registro:

Documento: Artículo
Título:Concerted stimulation of rat granulosa cell deoxyribonucleic acid synthesis by sex steroids and follicle-stimulating hormone
Autor:Bley, M.A.; Saragüeta, P.E.; Barañao, J.L.
Filiación:Inst. de Biol. y Med. Exp. - CONICET, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Obligado 2490 (1428), Buenos Aires, Argentina
Palabras clave:3beta androstanediol; androstanolone; androstenedione; androsterone; antiandrogen; cyclic AMP derivative; estradiol; follitropin; hydroxyflutamide; insulin; sex hormone; somatomedin C; animal cell; article; cell culture; cell proliferation; controlled study; culture medium; DNA synthesis; dose response; female; granulosa cell; in vitro study; nonhuman; rat; signal transduction
Año:1997
Volumen:62
Número:1
Página de inicio:11
Página de fin:19
DOI: http://dx.doi.org/10.1016/S0960-0760(97)00021-6
Título revista:Journal of Steroid Biochemistry and Molecular Biology
Título revista abreviado:J. STEROID BIOCHEM. MOL. BIOL.
ISSN:09600760
CODEN:JSBBE
CAS:androstanolone, 521-18-6; androstenedione, 26264-53-9, 63-05-8; androsterone, 53-41-8; estradiol, 50-28-2; follitropin, 9002-68-0; hydroxyflutamide, 52806-53-8; insulin, 9004-10-8; somatomedin C, 67763-96-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v62_n1_p11_Bley

Referencias:

  • Rao, M.C., Midgley, A.R., Richards, J.S., Hormonal regulation of ovarian cell proliferation (1978) Cell, 14, pp. 71-78
  • Goldenberg, R.L., Vaitukaitis, J.L., Ross, G.T., Estrogen and follicle stimulating hormone interactions on follicle growth in rats (1972) Endocrinology, 90, pp. 1492-1498
  • Hsueh, A.J.W., Adashi, E.Y., Joncs, P.B.C., Welsh, T.H.J., Hormonal regulation of differentiation of cultured granulosa cells (1984) Endocr. Rev., 5, pp. 76-127
  • Hammond, J.M., English, H.F., Regulation of deoxyribonucleic acid synthesis in cultured porcine granulosa cells by growth factors and hormones (1987) Endocrinology, 120, pp. 1039-1046
  • Feng, P., Catt, K.J., Knecht, M., Transforming growth factor β regulates the inhibitory action of epidermal growth factor during granulosa cell differentiation (1986) J. Biol. Chem., 261, pp. 14167-14170
  • Dodson, W.C., Schomberg, D.W., The effect of transforming growth factor-β on follicle-stimulating hormone-induced differentiation of cultured rat granulosa cells (1987) Endocrinology, 120, pp. 512-516
  • Reilly, C.M., Cannady, W.E., Mahesh, V.B., Stopper, V.S., De Sevilla, L.M., Mills, T.M., Duration of estrogen exposure prior to follicle-stimulating hormone stimulation is critical to granulosa cell growth and differentiation in rats (1996) Biol. Reprod., 54, pp. 1336-1342
  • Greenwald, G.S., Roy, S.K., Follicular development and its control (1994) The Physiology of Reproduction, 1, pp. 629-724. , eds E. Knobil, J. D. Neill, G. S. Greenwald, C. L. Markert and D. W. Pfaff. Raven Press, New York
  • Bley, M.A., Simón, J.C., Saragüeta, P.E., Barañao, J.L., Hormonal regulation of rat granulosa cell deoxyribonucleic acid synthesis: Effects of estrogens (1991) Biol. Reprod., 44, pp. 880-888
  • Bley, M.A., Simón, J.C., Estévez, A.G., Jiménez De Asúa, L., Barañao, J.L., Effect of follicle-stimulating: Hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis (1992) Endocrinology, 131, pp. 1223-1229
  • Dain, L.B., Bley, M.A., Barañao, J.L., Tesone, M., Evidence for the production of a growth-inhibitory factor by human granulosa-luteal cells (1993) Molec. Reprod. Dev., 36, pp. 159-163
  • Magoffin, D.A., Erickson, G.F., Purification of ovar an theca-interstitial cells by density gradient centrifugation (1988) Endocrinology, 122, pp. 2345-2347
  • Barañao, J.L., Bley, M.A., Batista, F.D., Glikin, G.C., A DNA topoisomerase I inhibitor blocks the differentiation of rat granulosa cells induced by follicle-stimulating hormone (1991) Biochem. J., 277, pp. 557-560
  • Li, C.C., (1964) Introduction to Experimental Statistics, , McGraw-Hill, New York
  • DeLean, A., Munson, P.J., Rodbard, D., Simultaneous analysis of families of sigmoidal curves: Application to bioassay, radioligand assay and physiological dose-response curves (1978) Am. J. Physiol., 235, pp. E97-E102
  • Mowsowicz, I., Bieber, D.E., Chung, K.W., Bullock, L.P., Bardin, C.W., Synandrogenic and antiandrogenic effect of progestins: Comparison with non-progestational antiandrogens (1974) Endocrinology, 95, pp. 1589-1599
  • Park, K.C., Kim, S.J., Bang, Y.J., Park, J.G., Kim, N.K., Roberts, A.B., Sporn, M.B., Genetic changes in the transforming growth factor β (TGF-β) type II receptor gene in human gastric cancer cells: Correlation with sensitivity to growth inhibition by TGF-β (1994) Proc. Natl. Acad. Sci. U.S.A., 91, pp. 8772-8776
  • Kasson, B.G., Meidan, R., Davoren, B., Hsueh, A.J.W., Identification of subpopulations of rat granulosa cells: Sedimentation properties and hormonal responsiveness (1985) Endocrinology, 117, pp. 1027-1034
  • Rao, I.M., Mills, T.M., Anderson, E., Mahesh, V.B., Heterogeneity in granulosa cells of developing rat follicles (1991) Anat. Rec., 229, pp. 177-185
  • Gospodarowicz, D., Bialecki, H., Fibroblast and epidermal growth factors are mitogenic for granulosa cells of rodent, porcine and human origin (1979) Endocrinology, 104, pp. 409-420
  • Epstein-Almog, R., Orly, J., Inhibition of hormone-induced steroidogenesis during cell proliferation in serum-free cultures of rat granulosa cells (1985) Endocrinology, 116, pp. 2103-2112
  • Dorrington, J.H., Chuma, A.V., Bendell, J.J., Transforming growth factor β and follicle stimulating hormone promote rat granulosa cell proliferation (1988) Endocrinology, 123, pp. 353-359
  • Hirshfield, A.N., Flickinger, G.L., Ben-Rafael, Z., Flow cytofluorometric analysis granulosa cell proliferation in rats (1995) J. Reprod. Fertil., 84, pp. 231-238
  • Van Weissenbruch, M.M., Drexhage, H.A., Van Vliet-Bleeker, I., Schoemaker, J., Deoxyribonucleic acid cytophometry on rat ovarian follicles kept in organ culture: The effects of gonadotropins and plasma from normal, hypergonadotropic menopausal, and hypogonadotropic women (1990) J. Clin. Endocr. Metab., 70, pp. 238-245
  • Chakravorty, A., Mahesh, V.B., Mills, T.M., Inhibition by diethylstilboestrol of proliferative potential of follicles of different sizes in immature rat ovaries (1991) J. Reprod. Fertil., 92, pp. 323-332
  • Carroll, J., Whittingham, D.G., Wood, M.J., Effect of dibutyryl cyclic adenosine monophosphate on granulosa cell proliferation, oocyte growth and meiotic maturation in isolated mouse primary ovarian follicles cultured in collagen gels (1991) J. Reprod. Fertil., 92, pp. 197-207
  • McNatty, K.P., Sawers, R.S., Relationship between the endocrine environment whithin the Graafian follicle and the subsequent rate of progesterone secretion by human granulosa cells "in vitro" (1975) J. Endocr., 66, pp. 391-400
  • Gore-Langton, R.E., Armstrong, D.T., Follicular steroidogenesis and its control (1988) The Physiology of Reproduction, pp. 331-385. , eds E. Knobil and J. Neill. Raven Press Ltd, New York
  • Billig, H., Furuta, I., Hsueh, A.J.W., Estrogens inhibit and androgens enhance ovarian granulosa cell apoptosis (1993) Endocrinology, 133, pp. 2204-2212
  • Wang, X.-N., Greenwald, G.S., Synergistic effects of sex steroids with FSH on folliculogenesis, steroidogenesis and FSH- and hCG-receptors in hypophysectomized mice (1993) J. Reprod. Fertil., 99, pp. 403-413
  • Goldring, N.B., Orly, J., Concerted metabolism of steroids hormones produced by cocultured ovarian cells types (1985) J. Biol. Chem., 260, pp. 913-921
  • Magoffin, D.A., Erickson, G.F., Primary culture of differentiating ovarian androgen-producing cells in defined medium (1982) J. Biol. Chem., 257, pp. 4507-4513
  • Lüthy, I.A., Begin, D.J., Labrie, F., Androgenic activity of synthetic progestins and spironolactone in androgen-sensitive mouse mammmary carcinoma (Shinogi) cells in culture (1988) J. Steroid Biochem., 31, pp. 845-852
  • Bendell, J.J., Dorrington, J., Estradiol-17β stimulates DNA synthesis in rat granulosa cells: Action mediated by transforming growth factor-β (1991) Endocrinology, 128, pp. 2663-2665
  • Barañao, J.L., Saragüeta, P.E., Colman Lerner, A., Effect of transforming growth factor beta (TGF-β) on granulosa cell DNA synthesis: Modulation by hormones, growth, factors and GnRH (1993) Endocr. Soc. 75th Ann. Meet., 491. , Abstr. 1762
  • Saragueta, P., Colman Lerner, A.A., Lanuza, G., Fischman, M.L., Barañao, J.L., Interactions between TGF-β and follistatin in granulosa cells (1996) Biol. Reprod., 54 (SUPPL. 1), p. 155
  • Li, R., Phillips, D.M., Mather, J.P., Activin promotes ovarian follicle development in vitro (1995) Endocrinology, 136, pp. 849-856
  • Miró, F., Hillier, S.G., Modulation of granulosa cell deoxyribonucleic acid synthesis and differentiation by activin (1996) Endocrinology, 137, pp. 464-468
  • Dumont, J.E., Jauniaux, J., Roger, P.P., The cyclic AMP-mediated stimulation of proliferation (1989) TIBS, 14, pp. 67-71
  • Frishman, G.N., Luciano, A.A., Peluso, J.J., Effect of the ratio of follicle-stimulating hormone to luteinizing hormone on rat granulosa cell proliferation and oestradiol-17β secretion (1992) Human Reprod., 7, pp. 1073-1078
  • Peluso, J.J., Pappalardo, A., White, B.A., Control of rat granulosa cell mitosis by phorbol ester-, cyclic AMP-, and estradiol-17β-dependent pathways (1993) Biol. Reprod., 49, pp. 416-422
  • Yong, E.L., Baird, D.T., Hillier, S.G., Mediation of gonadotrophin-stimulated growth and differentiation of human granulosa cells by adenosine-3′,5′-monophosphate: One molecule, two messages (1992) Clin. Endocr. (Oxford), 37, pp. 51-58
  • Fujimoto, N., Katzenellenbogen, B.S., Alteration in the agonist/antagonist balance of antiestrogens by activation of protein kinase A signalling pathways in breast cancer cells: Antiestrogen selectivity and promoter dependence (1994) Molec. Endocr., 8, pp. 296-304
  • Sartorius, C.A., Tung, L., Takimoto, G.S., Horwitz, K.B., Antagonist-occupied human progesterone receptors bound to DNA are functionally switched to transcriptional agonist by cAMP (1994) J. Biol. Chem., 268, pp. 9262-9266
  • Jung-Testas, I., Schumacher, M., Bugnard, H., Baulieu, E.-E., Stimulation of rat Schwann cell proliferation by estradiol: Synergism between the estrogen and cAMP (1993) Dev. Brain Res., 72, pp. 282-290

Citas:

---------- APA ----------
Bley, M.A., Saragüeta, P.E. & Barañao, J.L. (1997) . Concerted stimulation of rat granulosa cell deoxyribonucleic acid synthesis by sex steroids and follicle-stimulating hormone. Journal of Steroid Biochemistry and Molecular Biology, 62(1), 11-19.
http://dx.doi.org/10.1016/S0960-0760(97)00021-6
---------- CHICAGO ----------
Bley, M.A., Saragüeta, P.E., Barañao, J.L. "Concerted stimulation of rat granulosa cell deoxyribonucleic acid synthesis by sex steroids and follicle-stimulating hormone" . Journal of Steroid Biochemistry and Molecular Biology 62, no. 1 (1997) : 11-19.
http://dx.doi.org/10.1016/S0960-0760(97)00021-6
---------- MLA ----------
Bley, M.A., Saragüeta, P.E., Barañao, J.L. "Concerted stimulation of rat granulosa cell deoxyribonucleic acid synthesis by sex steroids and follicle-stimulating hormone" . Journal of Steroid Biochemistry and Molecular Biology, vol. 62, no. 1, 1997, pp. 11-19.
http://dx.doi.org/10.1016/S0960-0760(97)00021-6
---------- VANCOUVER ----------
Bley, M.A., Saragüeta, P.E., Barañao, J.L. Concerted stimulation of rat granulosa cell deoxyribonucleic acid synthesis by sex steroids and follicle-stimulating hormone. J. STEROID BIOCHEM. MOL. BIOL. 1997;62(1):11-19.
http://dx.doi.org/10.1016/S0960-0760(97)00021-6