Artículo

González Deniselle, M.C.; González, S.; Piroli, G.; Ferrini, M.; Lima, A.E.; De Nicola, A.F. "Glucocorticoid receptors and actions in the spinal cord of the Wobbler mouse a model for neurodegenerative diseases" (1997) Journal of Steroid Biochemistry and Molecular Biology. 60(3-4):205-213
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Abstract:

We have studied glucocorticoid receptors (GR) and actions in the spinal cord of the Wobbler mouse, a model for amyotrophic lateral sclerosis and infantile spinal muscular atrophy. Basal and stress levels of circulating corticosterone (CORT) were increased in Wobbler mice. Single point binding assays showed that cytosolic type II GR in the spinal cord of Wobbler mice of both sexes were slightly reduced compared with normal littermates. Saturation analysis further demonstrated a non-significant reduction in B(max) with increased K(d). In the hippocampus, however, we found downregulation of GR, a probable response to increased CORT levels. We also found that the basal activity of ornithine decarboxylase (ODC), a rate-limiting enzyme of polyamine biosynthesis, was higher in Wobbler mice than in control animals. Both groups showed a two-fold stimulation of ODC activity after treatment with dexamethasone (DEX). Additionally, Wobbler mice presented with an intense proliferation of astrocytes immunoreactive (ir) for glial fibrillary acidic protein (GFAP) in grey and white matter of the spinal cord. The enhanced GFAP-ir was attenuated after four days of treatment with a corticosterone (CORT) pellet implant, producing a pharmacological increase in peripheral circulating CORT. Taking into consideration the content of GR and the changes in ODC activity and GFAP-ir brought about by glucocorticoids, we suggest that Wobbler mice are hormone responsive. Further elucidation of glucocorticoid effects in this model may be relevant for understanding the possible use of hormones in human neurodegenerative diseases.

Registro:

Documento: Artículo
Título:Glucocorticoid receptors and actions in the spinal cord of the Wobbler mouse a model for neurodegenerative diseases
Autor:González Deniselle, M.C.; González, S.; Piroli, G.; Ferrini, M.; Lima, A.E.; De Nicola, A.F.
Filiación:Lab. of Neuroendocrine Biochemistry, Inst. de Biol. y Med. Exp.-CONICET, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Depto. de Bioquimica Humana, Facultad de Medicina, University of Buenos Aires, Buenos Aires, Argentina
Palabras clave:corticosterone; dexamethasone; glial fibrillary acidic protein; glucocorticoid receptor; ornithine decarboxylase; amyotrophic lateral sclerosis; animal experiment; article; controlled study; degenerative disease; female; hereditary spinal muscular atrophy; hippocampus; male; mouse; nonhuman; receptor down regulation; spinal cord; Amyotrophic Lateral Sclerosis; Animals; Astrocytes; Cell Division; Corticosterone; Dexamethasone; Disease Models, Animal; Female; Glial Fibrillary Acidic Protein; Glucocorticoids; Lumbosacral Region; Male; Mice; Mice, Mutant Strains; Neck; Ornithine Decarboxylase; Receptors, Glucocorticoid; Sex Factors; Spinal Cord; Spinal Muscular Atrophies of Childhood
Año:1997
Volumen:60
Número:3-4
Página de inicio:205
Página de fin:213
DOI: http://dx.doi.org/10.1016/S0960-0760(96)00193-8
Título revista:Journal of Steroid Biochemistry and Molecular Biology
Título revista abreviado:J. STEROID BIOCHEM. MOL. BIOL.
ISSN:09600760
CODEN:JSBBE
CAS:Corticosterone, 50-22-6; Dexamethasone, 50-02-2; Glial Fibrillary Acidic Protein; Glucocorticoids; Ornithine Decarboxylase, EC 4.1.1.17; Receptors, Glucocorticoid
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v60_n3-4_p205_GonzalezDeniselle

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Citas:

---------- APA ----------
González Deniselle, M.C., González, S., Piroli, G., Ferrini, M., Lima, A.E. & De Nicola, A.F. (1997) . Glucocorticoid receptors and actions in the spinal cord of the Wobbler mouse a model for neurodegenerative diseases. Journal of Steroid Biochemistry and Molecular Biology, 60(3-4), 205-213.
http://dx.doi.org/10.1016/S0960-0760(96)00193-8
---------- CHICAGO ----------
González Deniselle, M.C., González, S., Piroli, G., Ferrini, M., Lima, A.E., De Nicola, A.F. "Glucocorticoid receptors and actions in the spinal cord of the Wobbler mouse a model for neurodegenerative diseases" . Journal of Steroid Biochemistry and Molecular Biology 60, no. 3-4 (1997) : 205-213.
http://dx.doi.org/10.1016/S0960-0760(96)00193-8
---------- MLA ----------
González Deniselle, M.C., González, S., Piroli, G., Ferrini, M., Lima, A.E., De Nicola, A.F. "Glucocorticoid receptors and actions in the spinal cord of the Wobbler mouse a model for neurodegenerative diseases" . Journal of Steroid Biochemistry and Molecular Biology, vol. 60, no. 3-4, 1997, pp. 205-213.
http://dx.doi.org/10.1016/S0960-0760(96)00193-8
---------- VANCOUVER ----------
González Deniselle, M.C., González, S., Piroli, G., Ferrini, M., Lima, A.E., De Nicola, A.F. Glucocorticoid receptors and actions in the spinal cord of the Wobbler mouse a model for neurodegenerative diseases. J. STEROID BIOCHEM. MOL. BIOL. 1997;60(3-4):205-213.
http://dx.doi.org/10.1016/S0960-0760(96)00193-8