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Abstract:

The aim of the present study was to determine the long-term effects of insulin treatment on luteal cell function. For this purpose, superovulated prepubertal rats were treated with insulin (group I) or vehicle (group C) for 9 days. Serum progesterone (P4) levels were increased in the insulin-treated group (55 ± 10 vs 134 ± 31 ng/ml, P < 0.05). Isolated luteal cells were incubated 3 h, and P4 and 20α-hydroxy-progesterone (20α-OH-P) were measured in the incubation media. A decrease in P4 levels and an increase in 20α-OH-P values [P4 (ng/ml): C = 26.6 ± 0.3; I = 20 ± 2; 20α-OH-P (ng/ml): C = 62 ± 2; I: 120 ± 7; P < 0.01] were observed in group I. In addition, progestagen (P4 + 20α-OH-P) levels were higher in group I (C = 88 ± 2; I = 140 ± 9 ng/ml; P < 0.001). When cytochrome P450SCC contents were measured by immunoblotting, a marked increase was observed in luteal cells obtained from group I. LH receptor numbers were decreased in luteal cells isolated from group I (C = 388,834 ± 14,146; I = 303,057 ± 13,392 sites/cell; P < 0.001) with a concomitantly diminished LH responsiveness. It is concluded that in vivo treatment of superovulated rats with insulin increases luteal progestagen production by increasing the content of cytochrome P450SCC. © 1995.

Registro:

Documento: Artículo
Título:In vivo regulation of the steroidogenic activity of rat luteal cells by insulin
Autor:Stein, P.; Bussmann, L.E.; Tesone, M.
Filiación:Instituto de Biología y Medicina Experimental, CONICET Obligado 2490, Buenos Aires and Facultad de Ciencias Exactas y Naturales (UBA), 1428 Buenos Aires, Argentina
Palabras clave:20alpha dihydroprogesterone; cytochrome p450; insulin; luteinizing hormone receptor; progesterone; animal cell; animal experiment; article; controlled study; female; immunoblotting; luteal cell; nonhuman; rat; steroidogenesis; subcutaneous drug administration; Animal; Cholesterol Side-Chain Cleavage Enzyme; Corpus Luteum; Dihydroprogesterone; Dose-Response Relationship, Drug; Drug Interactions; Female; Insulin; Luteinizing Hormone; Ovulation; Progesterone; Puberty; Rats; Rats, Sprague-Dawley; Receptors, LH; Support, Non-U.S. Gov't
Año:1995
Volumen:52
Número:4
Página de inicio:329
Página de fin:335
DOI: http://dx.doi.org/10.1016/0960-0760(94)00182-L
Título revista:Journal of Steroid Biochemistry and Molecular Biology
Título revista abreviado:J. Steroid Biochem. Mol. Biol.
ISSN:09600760
CODEN:JSBBE
CAS:20alpha dihydroprogesterone, 145-14-2; cytochrome P450, 9035-51-2; insulin, 9004-10-8; progesterone, 57-83-0; Cholesterol Side-Chain Cleavage Enzyme, EC 1.14.15.6; Dihydroprogesterone, 145-14-2; Insulin, 11061-68-0; Luteinizing Hormone, 9002-67-9; Progesterone, 57-83-0; Receptors, LH
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v52_n4_p329_Stein

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Citas:

---------- APA ----------
Stein, P., Bussmann, L.E. & Tesone, M. (1995) . In vivo regulation of the steroidogenic activity of rat luteal cells by insulin. Journal of Steroid Biochemistry and Molecular Biology, 52(4), 329-335.
http://dx.doi.org/10.1016/0960-0760(94)00182-L
---------- CHICAGO ----------
Stein, P., Bussmann, L.E., Tesone, M. "In vivo regulation of the steroidogenic activity of rat luteal cells by insulin" . Journal of Steroid Biochemistry and Molecular Biology 52, no. 4 (1995) : 329-335.
http://dx.doi.org/10.1016/0960-0760(94)00182-L
---------- MLA ----------
Stein, P., Bussmann, L.E., Tesone, M. "In vivo regulation of the steroidogenic activity of rat luteal cells by insulin" . Journal of Steroid Biochemistry and Molecular Biology, vol. 52, no. 4, 1995, pp. 329-335.
http://dx.doi.org/10.1016/0960-0760(94)00182-L
---------- VANCOUVER ----------
Stein, P., Bussmann, L.E., Tesone, M. In vivo regulation of the steroidogenic activity of rat luteal cells by insulin. J. Steroid Biochem. Mol. Biol. 1995;52(4):329-335.
http://dx.doi.org/10.1016/0960-0760(94)00182-L