Abstract:
The aim of the present study was to determine the long-term effects of insulin treatment on luteal cell function. For this purpose, superovulated prepubertal rats were treated with insulin (group I) or vehicle (group C) for 9 days. Serum progesterone (P4) levels were increased in the insulin-treated group (55 ± 10 vs 134 ± 31 ng/ml, P < 0.05). Isolated luteal cells were incubated 3 h, and P4 and 20α-hydroxy-progesterone (20α-OH-P) were measured in the incubation media. A decrease in P4 levels and an increase in 20α-OH-P values [P4 (ng/ml): C = 26.6 ± 0.3; I = 20 ± 2; 20α-OH-P (ng/ml): C = 62 ± 2; I: 120 ± 7; P < 0.01] were observed in group I. In addition, progestagen (P4 + 20α-OH-P) levels were higher in group I (C = 88 ± 2; I = 140 ± 9 ng/ml; P < 0.001). When cytochrome P450SCC contents were measured by immunoblotting, a marked increase was observed in luteal cells obtained from group I. LH receptor numbers were decreased in luteal cells isolated from group I (C = 388,834 ± 14,146; I = 303,057 ± 13,392 sites/cell; P < 0.001) with a concomitantly diminished LH responsiveness. It is concluded that in vivo treatment of superovulated rats with insulin increases luteal progestagen production by increasing the content of cytochrome P450SCC. © 1995.
Registro:
Documento: |
Artículo
|
Título: | In vivo regulation of the steroidogenic activity of rat luteal cells by insulin |
Autor: | Stein, P.; Bussmann, L.E.; Tesone, M. |
Filiación: | Instituto de Biología y Medicina Experimental, CONICET Obligado 2490, Buenos Aires and Facultad de Ciencias Exactas y Naturales (UBA), 1428 Buenos Aires, Argentina
|
Palabras clave: | 20alpha dihydroprogesterone; cytochrome p450; insulin; luteinizing hormone receptor; progesterone; animal cell; animal experiment; article; controlled study; female; immunoblotting; luteal cell; nonhuman; rat; steroidogenesis; subcutaneous drug administration; Animal; Cholesterol Side-Chain Cleavage Enzyme; Corpus Luteum; Dihydroprogesterone; Dose-Response Relationship, Drug; Drug Interactions; Female; Insulin; Luteinizing Hormone; Ovulation; Progesterone; Puberty; Rats; Rats, Sprague-Dawley; Receptors, LH; Support, Non-U.S. Gov't |
Año: | 1995
|
Volumen: | 52
|
Número: | 4
|
Página de inicio: | 329
|
Página de fin: | 335
|
DOI: |
http://dx.doi.org/10.1016/0960-0760(94)00182-L |
Título revista: | Journal of Steroid Biochemistry and Molecular Biology
|
Título revista abreviado: | J. Steroid Biochem. Mol. Biol.
|
ISSN: | 09600760
|
CODEN: | JSBBE
|
CAS: | 20alpha dihydroprogesterone, 145-14-2; cytochrome P450, 9035-51-2; insulin, 9004-10-8; progesterone, 57-83-0; Cholesterol Side-Chain Cleavage Enzyme, EC 1.14.15.6; Dihydroprogesterone, 145-14-2; Insulin, 11061-68-0; Luteinizing Hormone, 9002-67-9; Progesterone, 57-83-0; Receptors, LH
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v52_n4_p329_Stein |
Referencias:
- Poretsky, On the paradox of insulin-induced hyperandrogenism in insulin-resistant states (1991) Endocr. Rev., 12, pp. 3-13
- Ciaraldi, El-Roeiy, Madar, Reichart, Olefsky, Yen, Cellular mechanisms of insulin resistance in polycystic ovarian syndrome (1992) J. Clin. Endocrinol. Metab., 75, pp. 577-583
- Barbieri, Ryan, Hyperandrogenism, insulin resistance, and acanthosis nigricans syndrome: a common endocrinopathy with distinct pathophysiologic features (1983) Am. J. Obstet. Gynecol., 147, pp. 90-101
- Barbieri, Smith, Ryan, The role of hyperinsulinemia in the pathogenesis of ovarian hyperandrogenism (1988) Fertil Steril, 50, pp. 197-212
- Poretsky, Kalin, The gonadotropic function of insulin (1987) Endocrine Rev., 8, pp. 132-141
- Adashi, Resnick, Svoboda, Van Wyk, A novel role for somatomedin-C in the cytodifferentiation of the ovarian granulosa cell (1984) Endocrinology, 115, pp. 1227-1229
- Taylor, Dons, Hernandez, Roth, Gordon, Insulin resistance associated with androgen excess in women with auto-antibodies to the insulin receptor (1982) Ann. Int. Med., 97, pp. 851-855
- Davoren, Hsueh, Insulin enhances FSH-stimulated steroidogenesis by cultured rat granulosa cells (1984) Molec. Cell. Endocrinol., 35, pp. 97-105
- Adashi, Resnick, Svoboda, Van Wyk, Somatomedin-C synergizes with follicle-stimulating hormone in the acquisition of progestin biosynthetic capacity by cultured rat granulosa cells (1985) Endocrinology, 116, pp. 2135-2142
- Barbieri, Makris, Ryan, Effects of insulin on steroidogenesis in cultured porcine ovarian theca (1983) Fertil. Steril., 40, pp. 237-241
- Veldhuis, Kolp, Toaff, Strauss, Demers, Mechanisms subserving the trophic actions of insulin on ovarian cells: in vitro studies using swine granulosa cells (1983) J. Clin. Invest., 72, pp. 1046-1057
- Barañao, Hammond, Multihormone regulation of steroidogenesis in cultured porcine granulosa cells: studies in serum-free medium (1985) Endocrinology, 116, pp. 2143-2151
- Savion, Liu, Laherty, Gospodarowicz, Factors controlling proliferation and progesterone production by bovine granulosa cells in serum-free medium (1981) Endocrinology, 109, pp. 409-420
- Garzo, Dorrington, Aromatase activity in human granulosa cells during follicular development and the modulation by follicle-stimulating hormone and insulin (1984) Am. J. Obstet. Gynecol., 148, pp. 657-663
- Mondschein, Schomberg, Growth factors modulate gonadotropin receptor induction in granulosa cell cultures (1981) Science, 211, pp. 1179-1180
- Adashi, Resnick, Svoboda, Van Wyk, Somatomedin-C enhances induction of luteinizing hormone receptors by follicle-stimulating hormone in cultured rat granulosa cells (1985) Endocrinology, 116, pp. 2369-2375
- Erickson, Casper, Hofeditz, Role of serum-free defined medium in regulation of LH receptor in cultured rat granulosa cells (1983) Molec. Cell. Endocrinol., 30, pp. 37-50
- Sanders, Midgley, Rat granulosa cell differentiation: an in vitro model (1982) Endocrinology, 111, pp. 614-624
- Ladenheim, Tesone, Charreau, Insulin action and characterization of insulin receptors in rat luteal cells (1984) Endocrinology, 115, pp. 752-756
- Saragüeta, Krimer, Charreau, Tesone, Insulin regulation of steroidogenic activity in rat culture luteal cells (1989) J. Steroid Biochem., 32, pp. 393-397
- Poretsky, Glover, Laumas, Kalin, Dunaif, The effects of experimental hyperinsulinemia on steroid secretion, ovarian [125I]insulin binding, and ovarian [125I]insulin-like growth-factor I binding in the rat (1988) Endocrinology, 122, pp. 581-585
- Veldhuis, Rodgers, Dee, Simpson, The insulin-like growth factor, somatomedin-C, induces the synthesis of cholesterol side-chain cleavage cytochrome P-450 and adrenodoxin in ovarian cells (1986) J. Biol. Chem., 261, pp. 2499-2502
- Goldring, Durica, Lifka, Hedin, Ratoosh, Miller, Orly, Richards, Cholesterol side-chain cleavage cytochrome P450 messenger ribonucleic acid: evidence for hormonal regulation in rat ovarian follicles and constitutive expression in corpora lutea (1987) Endocrinology, 120, pp. 1942-1950
- Magoffin, Weitsman, Effect of insulin-like growth factor-I on cholesterol side-chain cleavage cytochrome P450 messenger ribonucleic acid expression in ovarian thecainterstitial cells stimulated to differentiate in vitro (1993) Molec. Cell. Endocrinol., 96, pp. 45-51
- Aflalo, Meidan, The hormonal regulation of cholesterol side-chain cleavage cytochrome P450, adrenodoxin, and their messenger ribonucleic acid expression in bovine small-like and large-like luteal cells: Relationship with progesterone production (1993) Endocrinology, 132, pp. 410-416
- Tesone, Ladenheim, Chiauzzi, Charreau, Effect of prolactin on the steroidogenic response of rat luteal cells (1984) Molec. Cell. Endocrinol., 36, pp. 187-194
- Molinolo, Lanari, Charreau, Sanjuan, Dosne Pasqualini, Mouse mammary tumors induced by medroxy-progesterone acetate: immunohystochemistry and hormonal receptors (1987) J. Natn. Cancer. Inst., 79, pp. 1341-1350
- Charreau, Calvo, Tesone, Biella de Souza Valle, Barañao, Insulin regulation of Leydig cell luteinizing hormone receptors (1978) J. Biol. Chem., 253, pp. 2504-2506
- Moyle, Netburn, Cosgrove, Krieger, Bahl, HCG-Leydig cell functional binding kinetics (1980) Threshold and nonlinearity in response, 238, pp. E293-E302. , Am. J. Physiol
- Guerrero, Stein, Asch, Polak de Fried, Tesone, Effect of a gonadotropin-releasing hormone agonist on luteinizing hormone receptors and steroidogenesis in ovarian cells (1993) Fertil. Steril., 59, pp. 803-809
- Laemmli, Cleavage of structural proteins during the assembly of the head of the bacteriophage T4 (1970) Nature, 227, pp. 680-688
- Lowry, Rosebrough, Farr, Randall, Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275
- Abraham, Swerdloff, Tulchinsky, Odell, Radioimmunoassay of plasma progesterone (1971) J. Clin. Endocrinol. Metab., 32, pp. 619-624
- Tesone, Ladenheim, Oliveira-Filho, Chiauzzi, Foglia, Charreau, Ovarian disfunction in streptozotocin-induced diabetic rats (1983) Proc. Soc. Exp. Biol. Med., 174, pp. 123-130. , 2nd Edition
- Adashi, Resnick, D'Ercole, Svoboda, Van Wyk, Insulin-like growth factors as intravarian regulators of granulosa cell growth and function (1985) Endocr. Rev., 6, pp. 400-420
- Talavera, Menon, Studies on rat luteal cell response to insulin-like growth factor I (IGF-I): identification of a specific cell membrane receptor for IGF-I in the luteinized rat ovary (1991) Endocrinology, 129, pp. 1340-1346
- Parmer, Roberts, Jr., LeRoith, Adashi, Khan, Solan, Nelson, Gibori, Expression, action, and steroidal regulation of insulin-like growth factor-I (IGF-I) and IGF-I receptor in the rat corpus luteum: their differential role in the two cell populations forming the corpus luteum (1991) Endocrinology, 129, pp. 2924-2932
- Conti, Harwood, Hsueh, Dufau, Catt, Gonadotropin-induced loss of hormone receptors and desensitization of adenylate cyclase in the ovary (1976) J. Biol. Chem., 251, pp. 7729-7731
- Sen, Azhar, Menon, Receptor-mediated gonadotropin action in the ovary (1979) Desensitization of gonadotropin binding sites, activation of adenosine 3′,5′-cyclic monophosphate-dependent protein kinase(s), and regulation of steroidogenesis in the ovary, 254, pp. 5664-5671. , J. Biol. Chem
- LaPolt, Oikawa, Jia, Dargan, Hsueh, Gonadotropin-induced up- and down-regulation of rat ovarian LH receptor message levels during follicular growth, ovulation and luteinization (1990) Endocrinology, 126, pp. 3277-3279
- Lu, Peegel, Mosier, Menon, Loss of lutropin/human chorionic gonadotropin receptor messenger ribonucleic acid during ligand-induced down-regulation occurs post transcriptionally (1993) Endocrinology, 132, pp. 235-240
- Lakkakorpi, Pietilä, Aatsinki, Rajaniemi, Human chorionic gonadotrophin (CG)-induced down-regulation of the rat luteal LH/CG receptor results in part from the down-regulation of its synthesis, involving increased alternative processing of the primary transcript (1993) J. Molec. Endocrinol., 10, pp. 153-162
- Chen, Gao, Shelton, Hatmaker, Coordinated receptor regulation in ovarian granulosa cells (1987) Advances in Experimental Medicine and Biology, pp. 703-707. , V.B. Mahesh, D.S. Dhindsa, E. Anderson, S.P. Kalra, Plenum Press, NY
- Lane, Chen, Heterologous down-modulation of luteinizing hormone receptors by prolactin: a flow cytometry study (1991) Endocrinology, 128, pp. 1833-1840
- Hall, Cellular organization for steroidogenesis (1984) Int. Rev. Cytol., 86, pp. 53-95
- Lieberman, Greenfield, Wolfson, A heuristic proposal for understanding steroidogenic processes (1984) Endocrine Rev., 5, pp. 128-148
- Waterman, Simpson, Regulation of the biosynthesis of cytochromes P-450 involved in steroid hormone synthesis (1985) Molec. Cell. Endocrinol., 39, pp. 81-89
- DuBois, Simpson, Kramer, Waterman, Induction of synthesis of cholesterol side chain cleavage cytochrome P-450 by adrenocorticotropin in cultured bovine adrenocortical cells (1981) J. Biol. Chem., 256, pp. 7000-7005
- Veldhuis, Klase, Strauss, III, Hammond, Facilitative interactions between estradiol and luteinizing hormone in the regulation of progesterone production by cultured swine granulosa cells: relation to cellular cholesterol metabolism (1982) Endocrinology, 111, pp. 441-447
Citas:
---------- APA ----------
Stein, P., Bussmann, L.E. & Tesone, M.
(1995)
. In vivo regulation of the steroidogenic activity of rat luteal cells by insulin. Journal of Steroid Biochemistry and Molecular Biology, 52(4), 329-335.
http://dx.doi.org/10.1016/0960-0760(94)00182-L---------- CHICAGO ----------
Stein, P., Bussmann, L.E., Tesone, M.
"In vivo regulation of the steroidogenic activity of rat luteal cells by insulin"
. Journal of Steroid Biochemistry and Molecular Biology 52, no. 4
(1995) : 329-335.
http://dx.doi.org/10.1016/0960-0760(94)00182-L---------- MLA ----------
Stein, P., Bussmann, L.E., Tesone, M.
"In vivo regulation of the steroidogenic activity of rat luteal cells by insulin"
. Journal of Steroid Biochemistry and Molecular Biology, vol. 52, no. 4, 1995, pp. 329-335.
http://dx.doi.org/10.1016/0960-0760(94)00182-L---------- VANCOUVER ----------
Stein, P., Bussmann, L.E., Tesone, M. In vivo regulation of the steroidogenic activity of rat luteal cells by insulin. J. Steroid Biochem. Mol. Biol. 1995;52(4):329-335.
http://dx.doi.org/10.1016/0960-0760(94)00182-L