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Abstract:

We had previously reported that juvenile hormone III (JH III) and the JH analogue 2-(4-phenoxy phenoxy)-ethoxytetrahydropyran exert inhibitory effects on progesterone synthesis by blocking cAMP production in hCG-stimulated MA-10 Leydig tumor cells. In the present study, the effects of JH analogue upon the biosynthetic pathway of progesterone synthesis have been examined. Our results demonstrated that JH analogue inhibited progesterone production even in the presence of 20-hydroxycholesterol or 25-hydroxycholesterol. Furthermore, although JH analogue inhibited pregnenolone production in hCG-stimulated MA-10 cells the activity of the 3β-hydroxysteroid dehydrogenase (3β-HSD) was unaffected. These data suggest that JH analogue might inhibit the steroidogenic pathway in Leydig tumor cells by inhibiting the activity of the cholesterol side chain cleavage (CSCC) enzymatic complex. The JH analogue was also evaluated for inhibitory actions on cholesterol availability. An important effect of this compound was the interference with the cellular process of plasma membrane cholesterol internalization. Moreover, JH analogue inhibited not only the use of cholesterol ester for steroid biosynthesis under Bt2cAMP stimulation, but also the cholesterol ester hydrolase (CEH) activity in MA-10 Leydig tumor cells. © 1995.

Registro:

Documento: Artículo
Título:Regulation of the cholesterol ester cycle and progesterone synthesis by juvenile hormone in MA-10 Leydig tumor cells
Autor:Vladusic, E.A.; Pignataro, O.P.; Bussmann, L.E.; Charreau, E.H.
Filiación:Instituto de Biología y Medicina Experimental (IBYME)-CONICET Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:2 (4 phenoxyphenoxy)ethoxytetrahydropyran; 20 hydroxycholesterol; 25 hydroxycholesterol; 3 decyldimethylsilyl n [2 (4 methylphenyl) 1 phenylethyl]propionamide; 3(or 17)beta hydroxysteroid dehydrogenase; aminoglutethimide; bucladesine; cholesterol esterase; cholesterol monooxygenase (side chain cleaving); chorionic gonadotropin; enzyme inhibitor; juvenile hormone derivative; pregnenolone; unclassified drug; animal cell; article; cholesterol transport; controlled study; enzyme activity; leydig cell tumor; male; nonhuman; progesterone synthesis; steroidogenesis; Bucladesine; Cell Membrane; Cholesterol; Cholesterol Esterase; Cholesterol Esters; Hydroxycholesterols; Juvenile Hormones; Leydig Cell Tumor; Progesterone; Pyrans; Sesquiterpenes; Support, Non-U.S. Gov't
Año:1995
Volumen:52
Número:1
Página de inicio:83
Página de fin:90
DOI: http://dx.doi.org/10.1016/0960-0760(94)00149-G
Título revista:Journal of Steroid Biochemistry and Molecular Biology
Título revista abreviado:J. Steroid Biochem. Mol. Biol.
ISSN:09600760
CODEN:JSBBE
CAS:20 hydroxycholesterol, 29913-75-5; 25 hydroxycholesterol, 2140-46-7; 3 decyldimethylsilyl n [2 (4 methylphenyl) 1 phenylethyl]propionamide, 78934-83-5; 3(or 17)beta hydroxysteroid dehydrogenase, 9015-81-0; aminoglutethimide, 125-84-8; bucladesine, 16980-89-5, 362-74-3; cholesterol esterase, 9026-00-0; cholesterol monooxygenase (side chain cleaving), 37292-81-2; chorionic gonadotropin, 9002-61-3; pregnenolone, 145-13-1; 2-(4-phenoxyphenoxy)ethoxytetrahydropyran; 25-hydroxycholesterol, 2140-46-7; Bucladesine, 362-74-3; Cholesterol Esterase, EC 3.1.1.13; Cholesterol Esters; Cholesterol, 57-88-5; Hydroxycholesterols; juvenile hormone III, 5255-04-9; Juvenile Hormones; Progesterone, 57-83-0; Pyrans; Sesquiterpenes
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v52_n1_p83_Vladusic

Referencias:

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Citas:

---------- APA ----------
Vladusic, E.A., Pignataro, O.P., Bussmann, L.E. & Charreau, E.H. (1995) . Regulation of the cholesterol ester cycle and progesterone synthesis by juvenile hormone in MA-10 Leydig tumor cells. Journal of Steroid Biochemistry and Molecular Biology, 52(1), 83-90.
http://dx.doi.org/10.1016/0960-0760(94)00149-G
---------- CHICAGO ----------
Vladusic, E.A., Pignataro, O.P., Bussmann, L.E., Charreau, E.H. "Regulation of the cholesterol ester cycle and progesterone synthesis by juvenile hormone in MA-10 Leydig tumor cells" . Journal of Steroid Biochemistry and Molecular Biology 52, no. 1 (1995) : 83-90.
http://dx.doi.org/10.1016/0960-0760(94)00149-G
---------- MLA ----------
Vladusic, E.A., Pignataro, O.P., Bussmann, L.E., Charreau, E.H. "Regulation of the cholesterol ester cycle and progesterone synthesis by juvenile hormone in MA-10 Leydig tumor cells" . Journal of Steroid Biochemistry and Molecular Biology, vol. 52, no. 1, 1995, pp. 83-90.
http://dx.doi.org/10.1016/0960-0760(94)00149-G
---------- VANCOUVER ----------
Vladusic, E.A., Pignataro, O.P., Bussmann, L.E., Charreau, E.H. Regulation of the cholesterol ester cycle and progesterone synthesis by juvenile hormone in MA-10 Leydig tumor cells. J. Steroid Biochem. Mol. Biol. 1995;52(1):83-90.
http://dx.doi.org/10.1016/0960-0760(94)00149-G