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Abstract:

The effect of a NADPH generating system (NADPH-GS) on the function of rat luteal cells was studied. Cells were obtained from pregnant mare serum gonadotropin (PMSG) and human choirionic gonadotropin (hCG) primed immature rats and further incubated with 3 NADPH-GS. This system produced an increase in progesterone production and maximal stimulation was achieved at 1 mM NADP+ (10- to 15-fold). This effect was enhanced by addition of luteinizing hormone (LH 0.25 nM) to the incubation medium. On the contrary, insulin (2 nM) inhibited the effect observed with the NADPH-GS. The conversion of progesterone into 20α-hydroxy-progesterone was not responsible for the changes observed. To analyze the site of NADPH action, pregnenolone and progesterone were measured using two inhibitors of steroid biosynthesis: aminoglutethimide and cyanoketone. The results confirm the specific site of action of NADPH at the mitochondrial conversion of cholesterol to pregnenolone. The effect of NADPH-GS was also observed in cultured purified luteal cells suggesting that the action of NADPH could be mediated by a free entry of the cofactor across the luteal cell plasma membrane. It can be concluded that the addition of NADPH improves the luteal cell incubation conditions and contributes to understanding the regulatory action of LH and insulin on the ovarian steroidogenic process. © 1992.

Registro:

Documento: Artículo
Título:Effect of a NADPH generating system on the steroidogenic response in rat luteal cells
Autor:Stein, P.; Tesone, M.
Filiación:Instituto de Biología y Medicina Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:20α-hydroxy-progesterone, 20α-hydroxy-4-pregnen-3-one; 25-hydroxy-cholesterol, 5-cholesten-3β-25-diol; pregnenolone; progesterone; reduced nicotinamide adenine dinucleotide phosphate; animal cell; article; female; luteal cell; nonhuman; priority journal; rat; steroidogenesis; Animal; Chorionic Gonadotropin; Corpus Luteum; Female; Gonadotropins, Equine; NADP; Pregnenolone; Progesterone; Rats; Support, Non-U.S. Gov't; Animalia
Año:1992
Volumen:41
Número:2
Página de inicio:145
Página de fin:150
DOI: http://dx.doi.org/10.1016/0960-0760(92)90041-G
Título revista:Journal of Steroid Biochemistry and Molecular Biology
Título revista abreviado:J. Steroid Biochem. Mol. Biol.
ISSN:09600760
CODEN:JSBBE
CAS:Chorionic Gonadotropin; Gonadotropins, Equine; NADP, 53-59-8; Pregnenolone, 145-13-1; Progesterone, 57-83-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v41_n2_p145_Stein

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Citas:

---------- APA ----------
Stein, P. & Tesone, M. (1992) . Effect of a NADPH generating system on the steroidogenic response in rat luteal cells. Journal of Steroid Biochemistry and Molecular Biology, 41(2), 145-150.
http://dx.doi.org/10.1016/0960-0760(92)90041-G
---------- CHICAGO ----------
Stein, P., Tesone, M. "Effect of a NADPH generating system on the steroidogenic response in rat luteal cells" . Journal of Steroid Biochemistry and Molecular Biology 41, no. 2 (1992) : 145-150.
http://dx.doi.org/10.1016/0960-0760(92)90041-G
---------- MLA ----------
Stein, P., Tesone, M. "Effect of a NADPH generating system on the steroidogenic response in rat luteal cells" . Journal of Steroid Biochemistry and Molecular Biology, vol. 41, no. 2, 1992, pp. 145-150.
http://dx.doi.org/10.1016/0960-0760(92)90041-G
---------- VANCOUVER ----------
Stein, P., Tesone, M. Effect of a NADPH generating system on the steroidogenic response in rat luteal cells. J. Steroid Biochem. Mol. Biol. 1992;41(2):145-150.
http://dx.doi.org/10.1016/0960-0760(92)90041-G