Navegar

Colección

Datos Estadísticas

Artículo

Michelini, F.M.; Ramírez, J.A.; Berra, A.; Galagovsky, L.R.; Alché, L.E. "Anti-herpetic and anti-inflammatory activities of two new synthetic 22,23-dihydroxylated stigmastane derivatives" (2008) Journal of Steroid Biochemistry and Molecular Biology. 111(1-2):111-116
La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Stromal keratitis resulting from ocular infection with Herpes simplex virus type 1 (HSV-1) is a common cause of blindness. This report investigates the antiviral and anti-inflammatory properties of two new synthetic stigmastane analogs in the experimental model of HSV-1-induced ocular disease in mice. (22S,23S)-22,23-dihydroxystigmast-4-en-3-one (1) and (22S,23S)-22,23-dihydroxystigmasta-1,4-dien-3-one (2) exhibited anti-HSV-1 activity in vitro and ameliorated the signs of murine herpetic stromal keratitis (HSK), although none of the compounds showed antiviral activity in vivo. We discuss that the improvement of HSK could be due to an immunomodulatory effect of both compounds. © 2008 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Anti-herpetic and anti-inflammatory activities of two new synthetic 22,23-dihydroxylated stigmastane derivatives
Autor:Michelini, F.M.; Ramírez, J.A.; Berra, A.; Galagovsky, L.R.; Alché, L.E.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina
Departamento de Química Orgánica y UMYMFOR, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina
Laboratorio de Investigaciones Oculares, Departamento de Patología, Facultad de Medicina, Buenos Aires, Argentina
Palabras clave:Anti-inflammatory; Antiviral; HSV-1; Murine herpetic stromal keratitis; Stigmastane analogs; 22,23 dihydroxystigmast 4 en 3 one; 22,23 dihydroxystigmasta 1,4 dien 3 one; antiinflammatory agent; antivirus agent; unclassified drug; animal cell; animal experiment; animal model; article; controlled study; drug activity; drug cytotoxicity; drug inhibition; drug structure; drug synthesis; human; human cell; Human immunodeficiency virus 1; in vitro study; keratitis; male; mouse; nonhuman; Animals; Anti-Inflammatory Agents; Antiviral Agents; beta-Galactosidase; Cell Line; Cell Survival; Cercopithecus aethiops; Disease Models, Animal; Dose-Response Relationship, Drug; Epithelial Cells; Epithelium, Corneal; Formazans; Herpesvirus 1, Human; Humans; Inhibitory Concentration 50; Keratitis, Herpetic; Male; Mice; Mice, Inbred BALB C; Molecular Structure; Spectrophotometry; Stigmasterol; Tetrazolium Salts; Time Factors; Vero Cells; Human herpesvirus 1; Murinae; Mus
Año:2008
Volumen:111
Número:1-2
Página de inicio:111
Página de fin:116
DOI: http://dx.doi.org/10.1016/j.jsbmb.2008.05.005
Título revista:Journal of Steroid Biochemistry and Molecular Biology
Título revista abreviado:J. Steroid Biochem. Mol. Biol.
ISSN:09600760
CODEN:JSBBE
CAS:Anti-Inflammatory Agents; Antiviral Agents; beta-Galactosidase, EC 3.2.1.23; Formazans; MTT formazan, 23305-68-2; Stigmasterol, 83-48-7; Tetrazolium Salts
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v111_n1-2_p111_Michelini

Referencias:

  • Wagner, E.K., Bloom, D.C., Experimental investigation of herpes simplex virus latency (1997) Clin. Microbiol. Rev., 10 (3), pp. 419-443
  • Carr, D.J.J., Härle, P., Gebhardt, B.M., The immune response to ocular herpes simplex virus type 1 infection (2001) Exp. Biol. Med., 226 (5), pp. 353-366
  • Liu, T., Tang, Q., Hendricks, R.L., Inflammatory infiltration of the trigeminal ganglion after herpes simplex virus type 1 corneal infection (1996) J. Virol., 70 (1), pp. 264-271
  • Hendricks, R.L., (1997) An immunologist's view of herpes simplex keratitis: Thygeson Lecture Presented at the Ocular Microbiology and Immunology Group Meeting, vol. 16, pp. 503-506. , October 26
  • Thomas, J., Gangappa, S., Kanangat, S., Rouse, B.T., On the essential involvement of neutrophils in immunopathological disease-herpetic stromal keratitis (1997) J. Immunol., 158, pp. 1383-1391
  • Wachsman, M.B., Ramirez, J.A., Galagovsky, L.R., Coto, C.E., Antiviral activity of brassinosteroids derivatives against measles virus in cell cultures (2002) Antiviral Chem. Chemother., 13 (1), pp. 61-66
  • Wachsman, M.B., López, E., Ramírez, J.A., Galagovsky, L.R., Coto, C.E., Antiviral effect of brassinosteroids against herpes virus and arenaviruses (2000) Antiviral Chem. Chemother., 11 (1), pp. 71-77
  • Michelini, F.M., Ramírez, J.A., Berra, A., Galagovsky, L.R., Alché, L.E., In vitro and in vivo antiherpetic activity of three new synthetic brassinosteroid analogues (2004) Steroids, 69 (11-12), pp. 713-720
  • Michelini, F.M., Berra, A., Alché, L.E., The in vitro immunomodulatory activity of a synthetic brassinosteroid analogue would account for the improvement of herpetic stromal keratitis in mice (2008) J. Steroid Biochem. Mol. Biol., 108, pp. 164-170
  • Ramírez, J.A., Bruttomesso, A.C., Michelini, F.M., Acebedo, S.L., Alché, L.E., Galagovsky, L.R., Syntheses of immunomodulating androstanes and stigmastanes: comparison of their TNF-alpha inhibitory activity (2007) Bioorg. Med. Chem., 15 (24), pp. 7538-7544
  • Hill, R.A., (1991) Dictionary of Steroids, , Makin H.L.J., Kirk D.N., and Murphy G.M. (Eds), Chapman & Hall/CRC
  • Diebold, Y., Calonge, M., Enríquez de Salamanca, A., Callejo, S., Corrales, R., Sáez, V., Siemasko, K.F., Stern, M.E., Characterization of a spontaneously immortalized cell line (IOBA-NHC) from normal human conjunctiva (2003) Invest. Ophthalmol. Vis. Sci., 44 (10), pp. 4263-4274
  • Denizot, F., Lang, R., Rapid colorimetric assay for cell growth and survival (1986) J. Immunol. Methods, 89, pp. 271-277
  • Porzel, A., Marquandt, V., Adam, G., 1H and 13C analysis of brassinosteroids (1992) Magn. Reson. Chem., 30, pp. 651-658
  • Li, H., Zhang, J., Kumar, A., Zheng, M., Atherton, S.S., Yu, F.-S.X., Herpes simplex virus 1 infection induces the expression of proinflammatory cytokines, interferons and TLR7 in human corneal epithelial cells (2006) Immunology, 117 (2), pp. 167-176
  • Biswas, P.S., Rouse, B.T., Early events in HSV keratitis-setting the stage for a blinding disease (2005) Microb. Infect., 7 (4), pp. 799-810
  • Wickham, S., Carr, D.J.J., Molecular mimicry versus bystander activation: herpetic stromal keratitis (2004) Autoimmunity, 37 (5), pp. 393-397
  • J.A. Ramírez, A. Berra, F. Michelini, L. Galagovsky, L. Alché, Argentine Patent P 07 0103089 (2007); Sarangi, P.P., Kim, B., Kurt-Jones, E., Rouse, B.T., Innate recognition network driving Herpes Simplex Virus-induced corneal immunopathology: role of the Toll pathway in early inflammatory events in stromal keratitis (2007) J. Virol., 81 (20), pp. 11128-11138

Citas:

---------- APA ----------
Michelini, F.M., Ramírez, J.A., Berra, A., Galagovsky, L.R. & Alché, L.E. (2008) . Anti-herpetic and anti-inflammatory activities of two new synthetic 22,23-dihydroxylated stigmastane derivatives. Journal of Steroid Biochemistry and Molecular Biology, 111(1-2), 111-116.
http://dx.doi.org/10.1016/j.jsbmb.2008.05.005
---------- CHICAGO ----------
Michelini, F.M., Ramírez, J.A., Berra, A., Galagovsky, L.R., Alché, L.E. "Anti-herpetic and anti-inflammatory activities of two new synthetic 22,23-dihydroxylated stigmastane derivatives" . Journal of Steroid Biochemistry and Molecular Biology 111, no. 1-2 (2008) : 111-116.
http://dx.doi.org/10.1016/j.jsbmb.2008.05.005
---------- MLA ----------
Michelini, F.M., Ramírez, J.A., Berra, A., Galagovsky, L.R., Alché, L.E. "Anti-herpetic and anti-inflammatory activities of two new synthetic 22,23-dihydroxylated stigmastane derivatives" . Journal of Steroid Biochemistry and Molecular Biology, vol. 111, no. 1-2, 2008, pp. 111-116.
http://dx.doi.org/10.1016/j.jsbmb.2008.05.005
---------- VANCOUVER ----------
Michelini, F.M., Ramírez, J.A., Berra, A., Galagovsky, L.R., Alché, L.E. Anti-herpetic and anti-inflammatory activities of two new synthetic 22,23-dihydroxylated stigmastane derivatives. J. Steroid Biochem. Mol. Biol. 2008;111(1-2):111-116.
http://dx.doi.org/10.1016/j.jsbmb.2008.05.005