Abstract:
Stromal keratitis resulting from ocular infection with Herpes simplex virus type 1 (HSV-1) is a common cause of blindness. This report investigates the antiviral and anti-inflammatory properties of two new synthetic stigmastane analogs in the experimental model of HSV-1-induced ocular disease in mice. (22S,23S)-22,23-dihydroxystigmast-4-en-3-one (1) and (22S,23S)-22,23-dihydroxystigmasta-1,4-dien-3-one (2) exhibited anti-HSV-1 activity in vitro and ameliorated the signs of murine herpetic stromal keratitis (HSK), although none of the compounds showed antiviral activity in vivo. We discuss that the improvement of HSK could be due to an immunomodulatory effect of both compounds. © 2008 Elsevier Ltd. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | Anti-herpetic and anti-inflammatory activities of two new synthetic 22,23-dihydroxylated stigmastane derivatives |
Autor: | Michelini, F.M.; Ramírez, J.A.; Berra, A.; Galagovsky, L.R.; Alché, L.E. |
Filiación: | Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina Departamento de Química Orgánica y UMYMFOR, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina Laboratorio de Investigaciones Oculares, Departamento de Patología, Facultad de Medicina, Buenos Aires, Argentina
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Palabras clave: | Anti-inflammatory; Antiviral; HSV-1; Murine herpetic stromal keratitis; Stigmastane analogs; 22,23 dihydroxystigmast 4 en 3 one; 22,23 dihydroxystigmasta 1,4 dien 3 one; antiinflammatory agent; antivirus agent; unclassified drug; animal cell; animal experiment; animal model; article; controlled study; drug activity; drug cytotoxicity; drug inhibition; drug structure; drug synthesis; human; human cell; Human immunodeficiency virus 1; in vitro study; keratitis; male; mouse; nonhuman; Animals; Anti-Inflammatory Agents; Antiviral Agents; beta-Galactosidase; Cell Line; Cell Survival; Cercopithecus aethiops; Disease Models, Animal; Dose-Response Relationship, Drug; Epithelial Cells; Epithelium, Corneal; Formazans; Herpesvirus 1, Human; Humans; Inhibitory Concentration 50; Keratitis, Herpetic; Male; Mice; Mice, Inbred BALB C; Molecular Structure; Spectrophotometry; Stigmasterol; Tetrazolium Salts; Time Factors; Vero Cells; Human herpesvirus 1; Murinae; Mus |
Año: | 2008
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Volumen: | 111
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Número: | 1-2
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Página de inicio: | 111
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Página de fin: | 116
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DOI: |
http://dx.doi.org/10.1016/j.jsbmb.2008.05.005 |
Título revista: | Journal of Steroid Biochemistry and Molecular Biology
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Título revista abreviado: | J. Steroid Biochem. Mol. Biol.
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ISSN: | 09600760
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CODEN: | JSBBE
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CAS: | Anti-Inflammatory Agents; Antiviral Agents; beta-Galactosidase, EC 3.2.1.23; Formazans; MTT formazan, 23305-68-2; Stigmasterol, 83-48-7; Tetrazolium Salts
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v111_n1-2_p111_Michelini |
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Citas:
---------- APA ----------
Michelini, F.M., Ramírez, J.A., Berra, A., Galagovsky, L.R. & Alché, L.E.
(2008)
. Anti-herpetic and anti-inflammatory activities of two new synthetic 22,23-dihydroxylated stigmastane derivatives. Journal of Steroid Biochemistry and Molecular Biology, 111(1-2), 111-116.
http://dx.doi.org/10.1016/j.jsbmb.2008.05.005---------- CHICAGO ----------
Michelini, F.M., Ramírez, J.A., Berra, A., Galagovsky, L.R., Alché, L.E.
"Anti-herpetic and anti-inflammatory activities of two new synthetic 22,23-dihydroxylated stigmastane derivatives"
. Journal of Steroid Biochemistry and Molecular Biology 111, no. 1-2
(2008) : 111-116.
http://dx.doi.org/10.1016/j.jsbmb.2008.05.005---------- MLA ----------
Michelini, F.M., Ramírez, J.A., Berra, A., Galagovsky, L.R., Alché, L.E.
"Anti-herpetic and anti-inflammatory activities of two new synthetic 22,23-dihydroxylated stigmastane derivatives"
. Journal of Steroid Biochemistry and Molecular Biology, vol. 111, no. 1-2, 2008, pp. 111-116.
http://dx.doi.org/10.1016/j.jsbmb.2008.05.005---------- VANCOUVER ----------
Michelini, F.M., Ramírez, J.A., Berra, A., Galagovsky, L.R., Alché, L.E. Anti-herpetic and anti-inflammatory activities of two new synthetic 22,23-dihydroxylated stigmastane derivatives. J. Steroid Biochem. Mol. Biol. 2008;111(1-2):111-116.
http://dx.doi.org/10.1016/j.jsbmb.2008.05.005